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Chemokine Receptor Profiles of T Cells in Patients with Age-Related Macular Degeneration

변석호|신의철|정혜원|최영준|임대한
연세대학교의과대학 (Yonsei University College of Medicine)|2022.04|Yonsei Medical Journal|제63권 제4호|pp. 357-364|DOI 10.3349/ymj.2022.63.4.357

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cytokineT cellsAge-related macular degenerationchemokinechemokine receptoraqueous humor
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초록

Purpose: To evaluate the expression of multiple chemokine receptors in peripheral blood T cells from patients with age-relatedmacular degeneration (AMD). Materials and Methods: Peripheral blood mononuclear cells and/or aqueous humor were obtained from 24 AMD patients and24 age- and sex-matched healthy controls. Chemokine receptor expression on T cells from peripheral blood was determined bymulticolor flow cytometry. The levels of chemokines and cytokines in the aqueous humor from 12 AMD patients and six healthycontrols were assessed. Results: AMD patients had increased expressions of CCR4 in CD4+ T cells (p=0.007) and CRTh2 in CD8+ T cells (p=0.002), and decreasedexpressions of CXCR3 in CD4+ T cells (p=0.029) and CXCR3, CCR5, and CX3CR1 in CD8+ T cells (p=0.005, 0.019, and 0.007,respectively). Monocyte chemoattractant protein-1 levels were increased in the aqueous humor from AMD patients (p=0.018),while the levels of interleukin (IL)-4 and IL-22 were significantly decreased compared to controls (p=0.018 and 0.041, respectively). Conclusion: The chemokine receptor profiles of T cells are altered in AMD patients compared to healthy controls without noticeableassociations with chemokine levels in the aqueous humor. Further evaluation is needed to clarify the role of these alterationsin AMD pathogenesis.

Abstract

Purpose: To evaluate the expression of multiple chemokine receptors in peripheral blood T cells from patients with age-relatedmacular degeneration (AMD). Materials and Methods: Peripheral blood mononuclear cells and/or aqueous humor were obtained from 24 AMD patients and24 age- and sex-matched healthy controls. Chemokine receptor expression on T cells from peripheral blood was determined bymulticolor flow cytometry. The levels of chemokines and cytokines in the aqueous humor from 12 AMD patients and six healthycontrols were assessed. Results: AMD patients had increased expressions of CCR4 in CD4+ T cells (p=0.007) and CRTh2 in CD8+ T cells (p=0.002), and decreasedexpressions of CXCR3 in CD4+ T cells (p=0.029) and CXCR3, CCR5, and CX3CR1 in CD8+ T cells (p=0.005, 0.019, and 0.007,respectively). Monocyte chemoattractant protein-1 levels were increased in the aqueous humor from AMD patients (p=0.018),while the levels of interleukin (IL)-4 and IL-22 were significantly decreased compared to controls (p=0.018 and 0.041, respectively). Conclusion: The chemokine receptor profiles of T cells are altered in AMD patients compared to healthy controls without noticeableassociations with chemokine levels in the aqueous humor. Further evaluation is needed to clarify the role of these alterationsin AMD pathogenesis.

참고문헌 (40)

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