Insights into the in vitro germicidal activities of Acalypha indica
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Background and purpose: This study was carried out to learn more about the potential prophylactic or antibacterial activity of the plant Acalypha indica against selective pathogenic bacteria. Experimental: The test organisms were Sarcina lutea IFO 3232, Bacillus subtilis IFO 3026, Pseudomonas denitrificans, Escherichia coli IFO 3007, Klebsiella pneumoniae ATTC 10031, Xanthomonas campestris IAM 1671, and Proteus vulgaris. Leaf, stem, and bud powder of Acalypha indica were dissolved in various solvents, and the extracts were tested for antimicrobial activity through the disc diffusion method. GC-MS profiling was performed to characterize active chemical compounds in the essential oil of Acalypha indica. Results: The ethanol extract showed the highest activity against all bacteria, while the petroleum ether extract yielded the highest zone of inhibition against Proteus vulgaris (11.83 ± 1.75 mm). The minimum inhibitory concentration (MIC) of the ethyl acetate extract against Bacillus subtilis was 16 μg/mL. Phytochemical screening by GC-MS revealed a total of 12 bioactive compounds. Conclusion: Extracts of Acalypha indica may be useful in formulating and synthesizing new antibacterial drugs.
Abstract
Background and purpose: This study was carried out to learn more about the potential prophylactic or antibacterial activity of the plant Acalypha indica against selective pathogenic bacteria. Experimental: The test organisms were Sarcina lutea IFO 3232, Bacillus subtilis IFO 3026, Pseudomonas denitrificans, Escherichia coli IFO 3007, Klebsiella pneumoniae ATTC 10031, Xanthomonas campestris IAM 1671, and Proteus vulgaris. Leaf, stem, and bud powder of Acalypha indica were dissolved in various solvents, and the extracts were tested for antimicrobial activity through the disc diffusion method. GC-MS profiling was performed to characterize active chemical compounds in the essential oil of Acalypha indica. Results: The ethanol extract showed the highest activity against all bacteria, while the petroleum ether extract yielded the highest zone of inhibition against Proteus vulgaris (11.83 ± 1.75 mm). The minimum inhibitory concentration (MIC) of the ethyl acetate extract against Bacillus subtilis was 16 μg/mL. Phytochemical screening by GC-MS revealed a total of 12 bioactive compounds. Conclusion: Extracts of Acalypha indica may be useful in formulating and synthesizing new antibacterial drugs.
참고문헌 (30)
- A. S. Fauci, , Emerg. Infect. Dis, 11(4), pp.519~525 (2005)
- H. Westh, , Microb. Drug Resist, 10(2), pp.169~176 (2004)
- A. Afolayan, , Pharm. Biol, 41(1), pp.22~25 (2003)
- V. Gangadevi, , Indian J. Sci. Technol, 1(5), pp.1~6 (2008)
- V. Schulz, "Rational phytotherapy:a physician's guide to herbal medicine" (2001), Springer-Verlag Berlin Heidelberg
- T. Selvamohan, , Adv. Appl. Sci. Res, 3(5), pp.3374~3381 (2012)
- D. Jagatheeswari, , Int. J. Res. Bot, 1, pp.19~22 (2013)
- K. K. Behera, , Ethnobotanic. Leaflets, 10(1), pp.149~173 (2006)
- K. Sudharameshwari, , Afr. J. Tradit. Complement. Altern. Med, 4(2), pp.199~204 (2008)
- R. Saha, , Int. J. Pharm. Sci. Res, 2(11), pp.2934~2937 (2011)
- T. Sinha, , Int. J. Res. Pharm. Sci, 3, pp.360~368 (2012)
- S. Jain, , Ethnobot. Search New Drugs, 185, pp.153~168 (1994)
- J. S. Reddy, , J. Ethnopharmacol, 79(2), pp.249~251 (2002)
- S. Bhalerao, , Int. Res. J. Biol. Sci, 1(5), pp.79~84 (2012)
- P. Paindla, , Online Int. Interdiscip. Res. J, 4(1), pp.175~182 (2014)
- R. M. Kunwar, , J. Ethnobiol. Ethnomed, 6(1), pp.35~ (2010)
- A. Rahman, , Arab. J. Chem., 9(1), pp.S475~S479 (2016)
- A. Bauer, , Am. J. Clin. Pathol, 45(4), pp.493~496 (1966)
- S. Rahman, , Ann. Clin. Microbiol. Antimicrob, 10(1), pp.1~ (2011)
- Afef Be´jaoui, "Essential Oil Composition and Antibacterial Activity of Origanum vulgare subsp. glandulosum Desf. at Different Phenological Stages", Journal of Medicinal Food, 16(12), pp.1115~1120 (2013), 한국식품영양과학회[DOI]
- T. Cachet, , Flavour Frag. J, 26(5), pp.297~299 (2011)
- M. T. Peres, , J. Ethnopharmacol, 56(3), pp.223~226 (1997)
- A. Falodun, , J. Med. Plants Res, 2(12), pp.365~369 (2009)
- O. Irobi, , J. Ethnopharmacol, 43(3), pp.185~190 (1994)
- M. Somchit, , J. Ethnopharmacol, 84(1), pp.1~4 (2003)
- K. R. Durga, , J. Med. Plants Res, 3(10), pp.703~706 (2009)
- T. Murugan, , Int. J. Pharm. Biol. Sci. Res, 2(5), pp.1473~1478 (2011)
- M. Govindarajan, , Eur. Rev. Med. Pharmacol. Sci, 12(5), pp.299~302 (2008)
- J. A. Gansau, , J. Environ. Microbiol. Toxicol, 1(1), pp.18~24 (2014)
- S. Arunkumar, , World J. Agri. Sci, 5(5), pp.572~576 (2009)