Establishment and validation of an analytical method for quality control of health functional foods derived from Agastache rugosa
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Agastache rugosa, known as Korean mint, is a medicinal plant with many beneficial health effects. In this study, a simple and reliable HPLC-UV method was proposed for the quantification of rosmarinic acid (RA) in the aqueous extracts of A. rugosa. RA was selected as a quantification marker due to its easiness in procurement and analysis. The developed method involved chromatographic separation on a C18 column (250 × 4.6 mm, 5 μm) at room temperature. The mobile phase consisted of water and acetonitrile both containing 2 % acetic acid and was run at a flow rate of 1 mL min−1. The method was validated for specificity, linearity, precision, and accuracy. It was specific to RA and linear in the range of 50–300 μg mL−1 (r2 = 0.9994). Intraday, inter-day, and inter-analyst precisions were ≤ 0.91 % RSD, ≤ 1.40 % RSD, and 1.94 % RSD, respectively. Accuracy was 93.3–95.9 % (≤ 1.21 % RSD). The method could be applied to three batches of bulk samples and three batches of lab scale samples, which were found to be 0.64 (± 0.04) mg g−1 and 0.48 (± 0.02) mg g−1 for the dried raw materials of A. rugosa. The results show that the proposed method can be used as a readily applicable method for QC of health functional foods containing the aqueous extracts of A. rugosa.
Abstract
Agastache rugosa, known as Korean mint, is a medicinal plant with many beneficial health effects. In this study, a simple and reliable HPLC-UV method was proposed for the quantification of rosmarinic acid (RA) in the aqueous extracts of A. rugosa. RA was selected as a quantification marker due to its easiness in procurement and analysis. The developed method involved chromatographic separation on a C18 column (250 × 4.6 mm, 5 μm) at room temperature. The mobile phase consisted of water and acetonitrile both containing 2 % acetic acid and was run at a flow rate of 1 mL min−1. The method was validated for specificity, linearity, precision, and accuracy. It was specific to RA and linear in the range of 50–300 μg mL−1 (r2 = 0.9994). Intraday, inter-day, and inter-analyst precisions were ≤ 0.91 % RSD, ≤ 1.40 % RSD, and 1.94 % RSD, respectively. Accuracy was 93.3–95.9 % (≤ 1.21 % RSD). The method could be applied to three batches of bulk samples and three batches of lab scale samples, which were found to be 0.64 (± 0.04) mg g−1 and 0.48 (± 0.02) mg g−1 for the dried raw materials of A. rugosa. The results show that the proposed method can be used as a readily applicable method for QC of health functional foods containing the aqueous extracts of A. rugosa.
참고문헌 (28)
- Y. B. Kim, , PLoS One, 8(5), pp.e64199~ (2013)
- H. Gong, , J. Essent. Oil. Bear. Pl, 15(4), pp.534~538 (2012)
- Y. Oh, , J. Photochem. Photobiol. B : Biol, 163, pp.170~176 (2016)
- S. Shin, , Lett. Appl. Microbiol, 36(2), pp.111~115 (2003)
- Seungwon Shin, "Essential Oil Compounds from Agastache rugosa as Antifungal Agents Against Trichophyton Species", Archives of Pharmacal Research, 27(3), pp.295~299 (2004), 대한약학회
- 김종희, "Phytotoxic and Antimicrobial Activities and Chemical Analysis of Leaf Essential Oil from Agastache rugosa", Journal of Plant Biology(한국식물학회지), 51(4), pp.276~283 (2008), 한국식물학회[DOI]
- G. Haiyan, , Saudi. J. Biol. Sci, 23(4), pp.524~530 (2016)
- K. J. Park, , Pharm. Biol, 40(3), pp.189~195 (2002)
- H. K. Kim, , Arch. Pharmacal Res, 22(5), pp.520~523 (1999)
- P. Cao, , BMC Complement. Altern. Med, 17(1), pp.93~ (2017)
- K. T. Desta, , Biomed. Chromatogr, 30(2), pp.225~231 (2016)
- J. -J. Hong, , FEBS Lett, 495(3), pp.142~147 (2001)
- H. Q. Li, , Molecules, 18(4), pp.4170~4180 (2013)
- I. A. S. V. Packiavathy, , Food Res. Int, 45(1), pp.85~92 (2012)
- G. Ruberto, , Food Chem, 69(2), pp.167~174 (2000)
- K. P. Devi, , J. Ethnopharmacol, 130(1), pp.107~115 (2010)
- S. Zielińska, , Phytochem. Rev, 13(2), pp.391~416 (2014)
- T. Hamaguchi, , Am. J. Pathol, 175(6), pp.2557~2565 (2009)
- V. Swarup, , Antimicrob. Agents Chemother, 51(9), pp.3367~ (2007)
- J. Lee, , Br. J. Pharmacol, 148(3), pp.366~375 (2006)
- O. Hernández-Abreu, , J. Ethnopharmacol, 138(2), pp.487~491 (2011)
- O. Hernández-Abreu, , J. Ethnopharmacol, 146(1), pp.187~191 (2013)
- S. Li, , Chin. Med, 3(1), pp.7~ (2008)
- Yan Jin, "A simple and reliable analytical method based on HPLC–UV to determine oleanonic acid content in Chios gum mastic for quality control", Archives of Pharmacal Research, 40(1), pp.49~56 (2017), 대한약학회
- H. M. Oh, , J. Ethnopharmacol, 103(2), pp.229~235 (2006)
- N. Erkan, , Food Chem, 110(1), pp.76~82 (2008)
- H. P. Bais, , Plant. Physiol. Bioch, 40(11), pp.983~995 (2002)
- Y. Jin, , Molecules, 24(9), pp.pii~E1735 (2019)