Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at various developmental stages of parasites, including asexual, sexual stages, and oocysts. The amount of antigen-stimulating IgG antibodies was particularly high on the oocyst wall. The sporozoite surface was shown to give stimulation on IgG and IgM antibody formation. Trophozoites implicated the lowest antigenicity to humoral immunity, both IgG and IgM, by showing the least amount of gold labeling. Immunogold labeling also provided clues that antigens were presented to the host-cell cytoplasm via feeder organelles and host-parasite junctions.
Abstract
The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at various developmental stages of parasites, including asexual, sexual stages, and oocysts. The amount of antigen-stimulating IgG antibodies was particularly high on the oocyst wall. The sporozoite surface was shown to give stimulation on IgG and IgM antibody formation. Trophozoites implicated the lowest antigenicity to humoral immunity, both IgG and IgM, by showing the least amount of gold labeling. Immunogold labeling also provided clues that antigens were presented to the host-cell cytoplasm via feeder organelles and host-parasite junctions.
참고문헌 (14)
- Fayer R, "Cryptosporidium and cryptosporidiosis", pp.1~41 (1997), CRC Press
- Cho MH, "Detection of Cryptosporidium oocysts from out-patients of the Severance Hospital", Korea. Korean J Parasitol, 31, pp.193~199 (1993)
- Chai JY, "An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do", Korean J Parasitol, 34, pp.113~119 (1996)
- 이종규, "Prevalenc of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea", The Korean Journal of Parasitology, 43(3), pp.111~114 (2005), 대한기생충학회
- Seo M, "An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun", Korean J Parasitol, 39, pp.201~203 (2001)
- 국상미, "Parasitic infections in HIV-infected patients who visited Seoul National University Hospital during the period 1995-2003", The Korean Journal of Parasitology, 43(1), pp.1~12 (2005), 대한기생충학회
- Fayer R, "Immunogold labeling of stages of Cryptosporidium parvum recognized by immunoglobulins in hyperimmune bovine colostrum", J Parasitol, 77, pp.487~490 (1991)
- Laxer MA, "Detection of Cryptosporidium parvum DNA in fixed, paraffin-embedded tissue by the polymerase chain reaction", Am J Trop Med Hyg, 47, pp.450~455 (1992)
- Yu JR, "Localization of actin and myosin in Cryptosporidium parvum using immunogold staining", Korean J Parasitol, 33, pp.155~164 (1995)
- Lumb R, "Localization of a 23,000 MW antigen of Cryptosporidium by immunoelectron microscopy", Immunol Cell Biol, 67(Pt 4), pp.267~270 (1989)
- Tilley M, "Identification of a 15-kilodalton surface glycoprotein on sporozoites of Cryptosporidium parvum", Infect Immun, 59, pp.1002~1007 (1991)
- Laxer MA, "Localization of human antibody binding sites on Cryptosporidium parvum oocysts and sporozoites by immunoelectron microscopy", Phil J Microbiol Infect Dis, 22, pp.41~49 (1993)
- Burns EA, "Decreased specific antibody synthesis in old adults: decreased potency of antigen-specific B cells with aging", Mech Ageing Dev, 53, pp.229~241 (1990)
- Elliott DA, "Cryptosporidium parvum induces host cell actin accumulation at the host-parasite interface", Infect Immun, 68, pp.2315~2322 (2000)