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In Vitro Effects of Some Herbs Used in Egyptian Traditional Medicine on Viability of Protoscolices of Hydatid Cysts

Doaa A. Yones|Gamal A. Taher|Zedan Z. Ibraheim
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2011.09|Parasites, Hosts and Diseases|제49권 제3호|pp. 255-263

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참고문헌

키워드

essential oilthymementholEchinococcus granulosusprotoscolexcystic echinococcussalviathymol
주제분류예방의학/직업환경의학

초록

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 μg/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 μg/ml) were used. Concentration of 2,500 μg/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 μg/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 μg/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 μg/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

Abstract

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 μg/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 μg/ml) were used. Concentration of 2,500 μg/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 μg/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 μg/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 μg/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

참고문헌 (46)

  1. McManus DP, "Echinococcosis", Lancet, 362, pp.1295~1304 (2003)
  2. Eckert J, "Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern", Clin Microbiol Rev, 17, pp.107~135 (2004)
  3. Mamuti W, "Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic echinococcosis in humans", Clin Diagn Lab Immunol, 9, pp.573~576 (2002)
  4. Eckert J, "Echinococcosis: An emerging or re-emerging zoonosis", Int J Parasitol, 30, pp.1283~1294 (2000)
  5. Haridy FM, "Hydatidosis granulosus in Egyptian slaughtered animals in the years 2000-2005", J Egypt Soc Parasitol, 36, pp.1087~1100 (2006)
  6. Sadjjadi SM, "Present situation of echinococcosis in the Middle East and Arabic North Africa", Parasitol Int, 55, pp.197~202 (2006)
  7. Frider B, "Treatment of liver hydatidosis: How to treat an asymptomatic carrier", World J Gastroenterol, 16, pp.4123~4129 (2010)
  8. Moazeni M, "High scolicidal effect of Zataria multiflora on protoscoleces of hydatid cyst: An in vitro study", Comp Clin Pathol, 580, pp.1068~1073 (2010)
  9. Reuter S, "Salvage treatment with amphotericin B in progressive human alveolar echinococcosis", Antimicrob Agents Chemother, 47, pp.3586~3591 (2003)
  10. Reuter S, "Structured treatment interruption in patients with alveolar echinococcosis", Hepatology, 39, pp.509~517 (2004)
  11. Deger E, "A new therapeutic approach for the treatment of cystic echinococcosis: Percutaneous albendazole sulphoxide injection without reaspiration", Am J Gastroenterol, 95, pp.248~254 (2000)
  12. Pessoa LM, "Anthelmintic activity of essential oil of Ocimum gratissimun Linn. and eugenol against Haemonchus contortus", Vet Parasitol, 109, pp.59~63 (2002)
  13. Elissondo MC, "Efficacy of thymol against Echinococcus granulosus protoscoleces", Parasitol Int, 57, pp.185~190 (2008)
  14. Ghazanfar SA, "Handbook of Arabian Medicinal Plants" (1994), Boca Raton, Florida, USA. CRC Press
  15. Akin M, "Antibacterial activity and composition of the essential oils of two endemic Salvia sp. from Turkey", Afr J Biotechnol, 9, pp.2322~2327 (2010)
  16. Karata H, "Antimicrobial activities of the essential oils of four Salvia species from Turkey", J Med Plant Res, 4, pp.1238~1240 (2010)
  17. Soković M, "Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model", Molecules, 15, pp.7532~7546 (2010)
  18. Moghadam MS, "Nejad SMS. Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime restistant Staphylococcus aureus strains", Asian Pac J Trop Med, 3, pp.262~265 (2010)
  19. Govaris A, "Antibacterial activity of oregano and thyme essential oils against Listeria monocytogenes and Escherichia coli O157:H7 in feta cheese packaged under modified atmosphere", LWT-Food Sci Technol, 44, pp.1240~1244 (2011)
  20. Maryam Behnia, "Inhibitory Effects of Iranian Thymus vulgaris Extracts in Vitro Growth of Entamoeba histolytica", The Korean Journal of Parasitology, 46(3), pp.153~156 (2008), 대한기생충학회
  21. Hotta M, "Carvacrol, a component of thyme oil, activates PPAR alpha and gamma and suppresses COX-2 expression", J Lipid Res, 51, pp.132~139 (2010)
  22. Smyth JD, "Studies on tapeworm physiology. XI. In vitro cultivation of Echinococcus granulosus from protoscolex to the strobilar stage", Parasitology, 57, pp.111~133 (1967)
  23. Kadir M, "Comparison between the efficacy of some chemical drugs and medical herbs on hydatid cysts", In Abstracts of the 11th Sci Cong Fac Vet Med, Assiut Univ, Egypt, pp.372~382 (2004)
  24. Smyth JD, "Procedures for testing the viability of human hydatid cysts following surgical removal especially after chemotherapy", Trans R Soc Trop Med Hyg, 74, pp.849~852 (1980)
  25. Balbaa SI, "Medicinal Plants Constituents. 3rd Ed" (1981), Cairo, Egypt. General Organization for University Books
  26. Trease GE, "Pharmacognosy. 11th ed", pp.42~55 (1978), London, England. Cassell and Collier Macmillan Publishers Ltd
  27. Harborne JB, "Phytochemical Methods", pp.49~188 (1973), London, England. Chapman and Hall
  28. Urrea-Paris M, "In vitro effect of praziquantel and albendazole combination therapy on the larval stage of Echinococcus granulosus", Parasitol Res, 86, pp.957~964 (2000)
  29. Moro P, "Echinococcosis: A review", Int J Infect Dis, 13, pp.125~133 (2009)
  30. "Effect of extracts of Zizphus spinachristi L. and Olea europaea L. on the larval stage of Echinococcus granulosus"[URL]
  31. Rassan AF, "A study on possibility of the attenuation of Echinococcus granulosus protoscolices by using various lasers rays", 박사, pp.1994
  32. Zhang W, "Concepts in immunology and diagnosis of hydatid disease", Clin Microbiol Rev, 16, pp.18~36 (2003)
  33. Yildirim A, "Statistical analyses of different staining methods for viability assessment in cystic echinococcosis", Turkiye Parazitol Derg, 31, pp.105~108 (2007)
  34. Junghanss T, "Clinical management of cystic echinococcosis: State of the art, problems, and perspectives", Am J Trop Med Hyg, 79, pp.301~ (2008)
  35. Barzinji A, "Effect of leaf extracts of Dendrosicyos socotrana and Jatropha unicostata on the viability of Echinococcus granulosus protoscoleces", Eur Asia J Bio Sci, 3, pp.122~129 (2009)
  36. Chopra I, "Uptake of minocycline by Escherichia coli", J Antimicrob Chemother, 29, pp.19~25 (1992)
  37. Walker M, "In vitro effects of nitazoxanide on Echinococcus granulosus protoscolices and metacestodes", J Antimicrob Chemother, 54, pp.609~616 (2004)
  38. Garg SC, "Anthelmintic activity of some medicinal plants", Hamdard Med, 40, pp.18~21 (1997)
  39. Hammond JA, "Prospects for plant anthelmintics in tropical veterinary medicine", Vet Res Commun, 21, pp.213~228 (1997)
  40. Cowan MM, "Plant products as antimicrobial agents", Clin Microbiol Rev, 12, pp.564~582 (1999)
  41. Bagambuola CF, "Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri", Food Microbiol, 21, pp.33~42 (2004)
  42. Jeyathilakan N, "Anthelmintic activity of essential oils of Cymbopogon nardus and Azadirachta indica on Fasciola gigantica", J Vet Animal Sci, 6, pp.204~209 (2010)
  43. Sánchez ME, "Surface activity of thymol: Implications for an eventual pharmacological activity", Colloids Surf B Biointerfaces, 34, pp.77~86 (2004)
  44. Sayek I, "Cystic hydatid disease: Current trends in diagnosis and management", Surg Today, 34, pp.987~996 (2004)
  45. Moazeni M, "In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst", World J Surg, 34, pp.2677~2681 (2010)
  46. Kismet K, "Evaluation on scolicidal efficacy of Propolis", Eur Surg Res, 38, pp.476~481 (2006)

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