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Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA

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Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by analyzing both the nuclear 18S small subunit ribosomal RNA (18S rRNA) and mitochondrial 16S rRNA gene loci, 27 clinical Acanthamoeba strains that caused AK in Japan were classified into 3 genotypes, T3 (3 strains), T4 (23 strains), and T5 (one strain). Most haplotypes were identical to the reference haplotypes reported from all over the world, and thus no specificity of the haplotype distribution in Japan was found. The T4 sub-genotype analysis using the 16S rRNA gene locus also revealed a clear sub-conformation within the T4 cluster, and lead to the recognition of a new sub-genotype T4i, in addition to the previously reported sub-genotypes T4a-T4h. Furthermore, 9 out of 23 strains in the T4 genotype were identified to a specific haplotype (AF479533), which seems to be a causal haplotype of AK. While heterozygous nuclear haplotypes were observed from 2 strains, the mitochondrial haplotypes were homozygous as T4 genotype in the both strains, and suggested a possibility of nuclear hybridization (mating reproduction) between different strains in Acanthamoeba. The nuclear 18S rRNA gene and mitochondrial 16S rRNA gene loci of Acanthamoeba spp. possess different unique characteristics usable for the genotyping analyses, and those specific features could contribute to the establishment of molecular taxonomy for the species complex of Acanthamoeba.

Abstract

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by analyzing both the nuclear 18S small subunit ribosomal RNA (18S rRNA) and mitochondrial 16S rRNA gene loci, 27 clinical Acanthamoeba strains that caused AK in Japan were classified into 3 genotypes, T3 (3 strains), T4 (23 strains), and T5 (one strain). Most haplotypes were identical to the reference haplotypes reported from all over the world, and thus no specificity of the haplotype distribution in Japan was found. The T4 sub-genotype analysis using the 16S rRNA gene locus also revealed a clear sub-conformation within the T4 cluster, and lead to the recognition of a new sub-genotype T4i, in addition to the previously reported sub-genotypes T4a-T4h. Furthermore, 9 out of 23 strains in the T4 genotype were identified to a specific haplotype (AF479533), which seems to be a causal haplotype of AK. While heterozygous nuclear haplotypes were observed from 2 strains, the mitochondrial haplotypes were homozygous as T4 genotype in the both strains, and suggested a possibility of nuclear hybridization (mating reproduction) between different strains in Acanthamoeba. The nuclear 18S rRNA gene and mitochondrial 16S rRNA gene loci of Acanthamoeba spp. possess different unique characteristics usable for the genotyping analyses, and those specific features could contribute to the establishment of molecular taxonomy for the species complex of Acanthamoeba.

참고문헌 (44)

  1. Anderson OR, "Laboratory and field-based studies of abundances, small-scale patchiness, and diversity of Gymnamoebae in soils of varying porosity and organic content : evidence of microbiocoenoses", J Eukaryot Microbiol, 49, pp.17~23 (2002)
  2. Hoffmann R, "Distribution of free-living amoebae(FLA)during preparation and supply of drinking water", Int J Hyg Envir Heal, 203, pp.215~219 (2001)
  3. Rodríguez-Zaragoza S, "Ecology of free-living amoebae", Crit Rev Microbiol, 20, pp.225~241 (1994)
  4. Jonckheere JF, "Species identification and virulence of Acanthamoeba strains from human nasal mucosa", Parasitol Res, 74, pp.314~316 (1988)
  5. Greub G, "Microorganisms resistant to free-living amoebae", Clin Microbiol Rev, 17, pp.413~433 (2004)
  6. Martinez AJ, "Free-living, amphizoic and opportunistic amebas", Brain Pathol, 7, pp.583~598 (1997)
  7. Seal DV, "Acanthamoeba keratitis update-incidence, molecular epidemiology and new drugs for treatment", Eye, 17, pp.893~905 (2003)
  8. Jones DB, "Acanthamoeba-the ultimate opportunist?", Am J Ophthalmol, 102, pp.527~530 (1986)
  9. Cheng KH, "Incidence of contact-lens-associated microbial keratitis and its related morbidity", Lancet, 354, pp.181~185 (1999)
  10. Stapleton F, "The incidence of contact lens-related microbial keratitis in Australia", Ophthalmology, 115, pp.1655~1662 (2008)
  11. Thebpatiphat N, "Acanthamoeba keratitis : a parasite on the rise", Cornea, 26, pp.701~706 (2007)
  12. Ishibashi Y, "Case of Acanthamoeba keratitis", Nippon Ganka Gakkai Zasshi, 92, pp.963~972 (1988)
  13. Stothard DR, "The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types", J Eukaryot Microbiol, 45, pp.45~54 (1998)
  14. Pussard M, "Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba(Protozoa, Amoebida)", Protistologica, 8, pp.557~598 (1977)
  15. Khan NA, "Acanthamoeba biology and pathogenesis" (2009), Caister Academic Press
  16. Schuster FL, "Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals", Int J Parasitol, 34, pp.1001~1027 (2004)
  17. Corsaro D, "Phylogenetic evidence for a new genotype of Acanthamoeba(Amoebozoa, Acanthamoebida)", Parasitol Res, 107, pp.233~238 (2010)
  18. Nuprasert W, "Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand", J Clin Microbiol, 48, pp.4636~4640 (2010)
  19. Schroeder JM, "Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge", J Clin Microbiol, 39, pp.1903~1911 (2001)
  20. Yu HS, "Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene", Korean J Parasitol, 37, pp.181~188 (1999)
  21. Ledee DR, "Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba", Invest Ophthalmol Vis Sci, 44, pp.1142~1149 (2003)
  22. Kobayashi A, "In vivo and ex vivo laser confocal microscopy findings in patients with early-stage Acanthamoeba keratitis", Cornea, 27, pp.439~445 (2008)
  23. Tamura K, "MEGA5 : Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol Biol Evol, 28, pp.2731~2739 (2011)
  24. Uno T, "Survey of severe contact lens-associated microbial keratitis in Japan", Nippon Ganka Gakkai Zasshi, 115, pp.107~115 (2011)
  25. Edagawa A, "Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan", Parasitol Res, 105, pp.1109~1117 (2009)
  26. Abe N, "Genotyping of Acanthamoeba isolates from corneal scrapings and contact lens cases of Acanthamoeba keratitis patients in Osaka, Japan", Jpn J Infect Dis, 63, pp.299~301 (2010)
  27. Inoue Y, "Multicenter molecular epidemiological study of clinical isolates related with Acanthamoeba keratitis(interim report)", Atarashii Ganka, 29, pp.397~402 (2012)
  28. Spanakos G, "Genotyping of pathogenic Acanthamoebae isolated from clinical samples in Greece-report of a clinical isolate presenting T5 genotype", Parasitol Int, 55, pp.147~149 (2006)
  29. Ledee DR, "Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients", J Clin Microbiol, 47, pp.1458~1462 (2009)
  30. Chung DI, "Subgenus classification of Acanthamoeba by riboprinting", Korean J Parasitol, 36, pp.69~80 (1998)
  31. 공현희, "Molecular Phylogeny of Acanthamoeba", The Korean Journal of Parasitology, 47, pp.21~28 (2009), 대한기생충학ㆍ열대의학회
  32. Gast RJ, "Subgenus systematics of Acanthamoeba : four nuclear 18S rDNA sequence types", J Eukaryot Microbiol, 43, pp.498~504 (1996)
  33. Lahr DJ, "The chastity of amoebae : re-evaluating evidence for sex in amoeboid organisms", Proceedings of the Royal Society B : Biological Sciences, 278, pp.2081~2090 (2011)
  34. Malik SB, "Protist homologs of the meiotic spo11 gene and topoisomerase VI reveal an evolutionary history of gene duplication and lineage-specific loss", Mol Biol Evol, 24, pp.2827~2841 (2007)
  35. Gunderson JH, "Length variation in eukaryotic rRNAs : small subunit rRNAs from the protists Acanthamoeba castellanii and Euglena gracilis", Gene, 44, pp.63~70 (1986)
  36. Lonergan KM, "The ribosomal RNA gene region in Acanthamoeba castellanii mitochondrial DNA : a case of evolutionary transfer of introns between mitochondria and plastids?", J Mol Biol, 239, pp.476~499 (1994)
  37. Ledee DR, "Acanthamoeba griffini. Molecular characterization of a new corneal pathogen", Invest Ophthalmol Vis Sci, 37, pp.544~550 (1996)
  38. Nagyová V, "Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia", Parasitol Res, 106, pp.861~872 (2010)
  39. Dupuy M, "Efficiency of water disinfectants against Legionella pneumophila and Acanthamoeba", Water Res, 45, pp.1087~1094 (2011)
  40. Booton GC, "Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections", J Clin Microbiol, 43, pp.1689~1693 (2005)
  41. Horn M, "Novel bacterial endosymbionts of Acanthamoeba spp. related to the Paramecium caudatum symbiont Caedibacter caryophilus", Environ Microbiol, 1, pp.357~367 (1999)
  42. Gast RJ, "Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype", J Eukaryot Microbiol, 48, pp.609~615 (2001)
  43. Hewett MK, "Identification of a new Acanthamoeba 18S rRNA gene sequence type, corresponding to the species Acanthamoeba jacobsi Sawyer, Nerad and Visvesvara, 1992(Lobosea : Acanthamoebidae)", Acta Protozool, 42, pp.325~329 (2003)
  44. Hua Liu, "Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses", The Korean Journal of Parasitology, 43(1), pp.1~23 (2005), 대한기생충학ㆍ열대의학회

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