Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice
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To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-α) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.
Abstract
To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-α) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.
참고문헌 (23)
- Lee KH, "Immunization of proteins from Toxascaris leonina adult worm inhibits allergic specific Th2 response", Vet Parasitol, 156, pp.216~225 (2008)
- Roberts LS, "Foundations of Parasitology" (2009), McGraw-Hill
- Nagy D, "Analysis of serum cytokine levels in children with chronic cough associated with Toxocara canis infection", Parasite Immunol, 34, pp.581~588 (2012)
- Nagy D, "Analysis of serum cytokine levels in children with chronic cough associated with Toxocara canis infection", Parasite Immunol, 34, pp.581~588 (2012)
- Takamoto M, "Eosinophilia, IgE production, and cytokine production by lung T cells in surface CD4-deficient mutant mice infected with Toxocara canis", Immunology, 95, pp.97~104 (1998)
- Torina A, "Toxocara canis infection induces antigen-specific IL-10 and IFN-gamma production in pregnant dogs and their puppies", Vet Immunol Immunopathol, 108, pp.247~251 (2005)
- 박혜경, "A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice", The Korean Journal of Parasitology, 49(4), pp.373~380 (2011), 대한기생충학ㆍ열대의학회
- 박미경, "Protease-Activated Receptor 2 Is Involved in Th2 Responses against Trichinella spiralis Infection", The Korean Journal of Parasitology, 49(3), pp.235~243 (2011), 대한기생충학ㆍ열대의학회
- Canto GJ, "The prevalence and abundance of helminth parasites in stray dogs from the city of Queretaro in central Mexico", J Helminthol, pp.1~7 (2011)
- 김용헌, "Prevalence of Toxocara canis, Toxascaris leonina and Dirofilaria immitis in dogs in Chuncheon, Korea (2004)", The Korean Journal of Parasitology, 43(2), pp.1~8 (2005), 대한기생충학ㆍ열대의학회
- Prokopic J, "The migration of larvae of Toxascaris leonina(Linstow, 1909)in experimentally infected white mice", Folia Parasitol(Praha), 29, pp.233~238 (1982)
- Maizels RM, "Susceptibility and immunity to helminth parasites", Curr Opin Immunol, 24, pp.459~466 (2012)
- Mathew A, "Signal transducer and activator of transcription 6 controls chemokine production and T helper cell type 2 cell trafficking in allergic pulmonary inflammation", J Exp Med, 193, pp.1087~1096 (2001)
- Sallusto F., "The role of chemokines and chemokine receptors in T cell priming and Th1", Th2-mediated responses, 1999, pp.84(suppl EHA-4), 28~31
- Okoshi S, "Experimental studies on Toxascaris leonina. VI. Experimental infection of mice, chickens and earthworms with Toxascaris leonina, Toxocara canis and Toxocara cati", Nippon Juigaku Zasshi, 30, pp.151~166 (1968)
- Bettelli E, "Induction and effector functions of T(H)17 cells", Nature, 453, pp.1051~1057 (2008)
- Yao R, "MicroRNA-155 modulates Treg and Th17 cell differentiation and Th17 cell function by targeting SOCS1", PLoS One, 7, pp.e46082~ (2012)
- Kang SA, "Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection", Vet Parasitol, 186, pp.319~327 (2012)
- Bazzone LE, "Coinfection with the intestinal nematode Heligmosomoides polygyrus markedly reduces hepatic egg-induced immunopathology and proinflammatory cytokines in mouse models of severe schistosomiasis", Infect Immun, 76, pp.5164~5172 (2008)
- Reece JJ, "Hookworm-induced persistent changes to the immunological environment of the lung", Infect Immun, 76, pp.3511~3524 (2008)
- Cheung PF, "Molecular mechanisms of cytokine and chemokine release from eosinophils activated by IL-17A, IL-17F, and IL-23 : implication for Th17 lymphocytes-mediated allergic inflammation", J Immunol, 180, pp.5625~5635 (2008)
- Akbari O, "Pulmonary dendritic cells producing IL-10 mediate tolerance induced by respiratory exposure to antigen", Nat Immunol, 2, pp.725~731 (2001)
- Presser K, "Coexpression of TGF-beta1 and IL-10 enables regulatory T cells to completely suppress airway hyperreactivity", J Immunol, 181, pp.7751~7758 (2008)