생쥐의 발정주기와 착상전, 후기 자궁내막에서p57kip2 단백질의 발현
The Expression of p57kip2 in Mouse Endometrium During Estrus Cycleand Pre- and Peri-implantation Periods
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목적 : 생쥐의 발정주기와 착상전, 후기 임신기간동안 자궁내막에서 CDK (cyclin-dependent kinase) inhibitor인 p57kip2 단백질의 발현양상을 알아보고자 하였다. 연구 방법 : 생쥐의 발정주기별, 그리고 임신 1-6일의 자궁내막 조직을 대상으로 면역조직화학 염색법을 이용하여 p57kip2 단백질의 발현양상을 조사하였다. 결과 : 발정말기에서 p57kip2 단백질은 일부 탈락막세포와 퇴화과정의 기질세포에서 이질적으로 강하게 발현되었다. 발정전기에서는 기질세포에서 전체적으로 강하게 발현되었으나, 발정기 시기에는 매우 미약하게 감소하였다. 발정후기에서는 탈락막화된 기질세포에서 강하게 발현되었다. 임신 1, 2일 시기에서는 기질세포에서 비교적 낮은 발현 양상을 보였으며, 임신 3, 4일 시기에서는 일부 기질세포에서 강하게 발현됨을 확인하였다. 착상기 시기인 임신 5, 6일 시기에서는 탈락막 세포에서 매우 강하게 발현되었다. 결론 : 본 연구 결과로 p57kip2는 생쥐의 발정주기와 착상을 위한 자궁내막의 분화에 중요한 역할을 할 것으로 사료되며, 특히 p57kip2는 착상의 유지를 위한 자궁내막 기질세포의 탈락막 반응에 핵심적인 역할을 할 것으로 사료된다.
Abstract
Objective : This study was to investigate the localization of CDK inhibitor, p57kip2 in mouse endometrium during the estrus cycle and pre- and peri-implantation periods. Methods : The p57kip2 protein was immunostained from endometrium of mouse sacrificed at diestrus, proestrus, estrus, and metestrus cycle, and at day 1-6 post-coitum (p.c.). Results : The staining in the luminal epithelium was very weak in comparison with glandular and stromal cells. In diestrus stage, immunoreactivity of p57kip2 was heterogeneously strong in parts of decidualized or degenerated stromal cells. In proestrus stage, strong immunoreactivity p57kip2 was largely found in stromal cells. But, p57kip2 was showed low immunoreactivity in estrus stage. In metestrus stage, immunoreactivity of p57kip2 was heterogeneously strong in decidualized stromal cells. In day 1-2 p.c., immunoreactivity of p57kip2 was low in some endometrial stromal cells. In day 3-4 p.c., immunoreactivity of p57kip2 was strong in some endometrial stromal cells. In day 5-6 p.c., immunoreactivity of p57kip2 was strong in decidual cells. Conclusion : These suggest that p57kip2 may play an essential role in endometrial differentiation for maintenance of implantation, especially decidualization of endometrial stromal cells.
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