자궁내막증 여성 난소 조직의 태아 기원microchimerism에 관한 연구
Microchimerism of Fetal Origin in Ovarian Tissues from Women with Pelvic Endometriosis
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
목적: 출산을 경험한 여성에게서 관찰되는 microchimerism이 흔한 부인과 질환이면서 자가면역질환의 특징을 가지는 자궁내막증 난소 조직에서 발견되는가를 알아보고자 하였다. 연구 방법: 환자군으로는 적어도 한 명 이상의 남자 아기를 출산한 산과력이 있는 여성에서 자궁내막증이 진단된 후 자궁내막종절제술 혹은 난소절제술을 시행한 환자 5명을 대상으로 하였으며, 대조군으로는 임신의 경력이 없는 여성에서 자궁내막증을 진단하고 자궁내막종절제술 혹은 난소절제술을 시행한 환자 5명을 대상으로 하였다. 태아 기원 세포의 존재 여부를 알아보기 위하여 X 염색체와 Y 염색체 특이 소식자 (probe)를 이용한 형광 제자리보합결합법 (fluorescence in situ hybridization, FISH)을 이용하여 조직 절편을 분석하였다. 결과: 파라핀 고정된 난소 조직에서 FISH가 성공적으로 시행되었다. 5명의 환자군 난소 조직 모두 태아측 남성 세포들이 발견되었다. 이와는 대조적으로 출산력이 없는 대조군의 경우에서는 5명 난소 조직 모두에서 태아 세포가 관찰되지 않았다. 결론: 또한, 임신 중 모체의 순환으로 이동된 태아측 microchimerism 세포가 자궁내막증과 같은 여성에서 호발하는 질환에 존재하고 있음이 확인되었다. 이러한 태아측 microchimerism 세포가 질병의 발생에 관련되는지에 대한 연구가 더욱 필요하다고 하겠다.
Abstract
Objective: To investigate whether fetal microchimeric cells were detected in ovarian tissues with pelvic endometriosis. Methods: Ovarian tissues with endometriosis were obtained from five women who had at least one live-born son and who underwent enucleation of endometriotic cyst or oophorectomy after a diagnosis of endometriotic cyst. Control tissues were obtained from five women with endometriosis who had no pregnant history. Tissue sections were analyzed with fluorescence in situ hybridization for the presence of fetal cells, defined by X and Y chromosome. Results: Fluorescence in situ hybridization using paraffin-embedded ovarian specimens was performed successfully. Male cells were found in ovarian tissues from all five patients. No male cells were found in ovarian tissues from all five controls. Conclusion: Fetal microchimeric cells, possibly from feto-maternal cell trafficking were detected in ovarian tissues with endometriosis were obtained from women who had prior male pregnancies. Further study is necessary to understand the role of persistent fetal microchimeric cells in the progression of endometriosis.
참고문헌 (27)
- Li geois A, "Michrochimerism:a stable state of low-ratio proliferation of allogeneic bone marrow", 9, pp.273~6 (1977)
- Agriga H, "Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy:implications for noninvasive prenatal diagnosis", 41, pp.1524~30 (2001)
- Bianchi DW, "Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum", 93, pp.705~8 (1996)
- Lo YM, "Detection of single-copy fetal DNA sequence from maternal blood", 335, pp.1463~4 (1990)
- Khosrotehrani K, "Transfer of fetal cells with multilineage potential to maternal tissue", 292, pp.75~80 (2004)
- Nelson JL, "Microchimerism and HLA-compatible relationships of pregnancy in scleroderma", 351, pp.559~62 (1998)
- Artlett CM, "Identification of fetal DNA and cells in skin lesions from women with systemic sclerosis", 338, pp.1186~91 (1998)
- Johnson KL, "Fetal cell microchimerism in tissue from multiple sites in women with systemic sclerosis", 44, pp.1848~54 (2001)
- Johnson KL, "Significant fetal cell microchimerism in a nontransfused woman with hepatitis C:Evidence of long-term survival and expansion", 36, pp.1295~7 (2002)
- Cha D, "Cervical cancer and microchimerism", 102, pp.774~81 (2003)
- Goldman MA, "The epidermiology of endometriosis", 323, pp.15~31 (1990)
- Koninckx PR, "Implantation versus infiltration:the Samson versus the endometriotic disease theory", 47, pp.3~9 (1999)
- Gleicher N, "Is endometriosis an autoimmune disease? Obstet Gynecol 1990", confi(e), pp.115~22
- Nothnick WB, "Treating endometriosis as an autoimmune disease", 76, pp.223~31 (2001)
- Johnson KL, "The use of fluorescence in situ hybridization on paraffin-embedded tissue sections for the study of microchimerism", 29, pp.1220~4 (2000)
- Johnson KL, "Fluorescence in situ hybridization", 73, pp.6~22 (1998)
- Srivatsa B, "Maternal cell microchimerism in newborn tissues", 142, pp.31~5 (2003)
- Lambert NC, "Male microchimerism in healthy women and women with scleroderma:cells or circulating DNA? a quantitative answer", 100, pp.2845~51 (2002)
- Miller FW, "Persistent maternally derived peripheral microchimerism is associated with juvenile idiopathic inflammatory myopathies", 40, pp.1279~84 (2001)
- Ando T, "Intrathyroidal fetal microchimerism in Graves' disease", 87, pp.3315~20 (2002)
- Klintschar M, "Evidence of fetal microchimerism in Hashimoto's thyroiditis", 86, pp.2494~8 (2001)
- Endo Y, "Possible contribution of microchimerism to the pathogenesis of Sjogren's syndrome", 41, pp.490~5 (2002)
- Fanning PA, "Detection of male DNA in the liver of female patients with primary biliary cirrhosis", 33, pp.690~5 (2000)
- Johnson KL, "Microchimerism in a female patient with systemic lupus erythematosus", 44, pp.2107~11 (2001)
- Aractingi S, "Fetal DNA in skin of polymorphic eruptions of pregnancy", 352, pp.1898~901 (1998)
- Srivatsa B, "Microchimerism of presumed fetal origin in thyroid specimens from women:A case-control study", 358, pp.2034~8 (2001)
- Catlin EA, "Transplacental transmission of natural killer-cell lymphoma", 341, pp.85~91 (1999)