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정상 산모와 중증 전자간증 산모의 태반에서 Matrix Metalloproteinase (MMP) -2, -9의 발현양상

Differential expression of Matrix Metalloproteinase (MMP)-2, -9 in normal and severe preeclamptic human placentas

박용원|황한성|김영한|권자영|문여정
대한산부인과학회 (Korean Soceity of Obstetrics and Gynecology)|2008.11|Obstetrics & Gynecology Science|제51권 제11호|pp. 1245-1253

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참고문헌

키워드

MMP-2MMP-9Placenta태반Severe preeclampsia중증 전자간증
주제분류산부인과학

초록

목적: 본 연구는 정상 태반 및 중증 전자간증 태반에서의 MMP-2와 MMP-9의 발현 차이를 비교하고자 하였다. 방법: 실험에 사용된 정상 및 중증 전자간증 산모의 태반은 만삭에 제왕절개술을 시행한 산모들을 대상으로 하였다. MMP-2,-9 의 유전자 발현 정도는 반정량적 RT-PCR을 통하여 측정되었다. 단백질의 정량화를 위하여 western blot을 시행하였고, 면역 학적 위치선정을 위하여 immunohistochemical staining을 시행하였다. 결과: RT-PCR 결과 MMP-2,-9의 유전자는 정상 및 중증 전자간증 태반 모두에서 발현되었고, 중증 전자간증에서 발현이 감소 되었다. Western blot을 시행한 결과 중증 전자간증에서 단백질 발현이 감소되었다. 면역조직화학 염색 결과 MMP-2,-9은 모 두 태반 내 융모막에서만 발현하였으며, 중증 전자간증 태반에서 발현이 감소하였다 결론: 중증 전자간증 산모의 태반에서 MMP-2,-9의 발현이 감소되어 있음을 확인하였으며, 본 연구를 통하여 MMP-2,-9이 중증 전자간증의 병인에 관여할 것으로 사료된다.

Abstract

Objective: The aim of the study was to investigate the differential expression of Matrix Metalloproteinase (MMP) -2,-9 in the normal and severe preeclamptic placenta. Methods: Placentas were obtained from women undergoing cesarean section with normal (n=10) and severe preeclamptic (n=10) pregnancies. Semi-quantitative RT-PCR was done to detect the placental gene expression of MMP-2,-9. Western blot analysis was performed to identify MMP-2,-9 protein expression in each placenta. Immunohistochemical staining were employed to localize MMP-2,-9 in placental tissues. Results: MMP-2,-9 genes were expressed in both normal and severe preeclamptic placenta. There were lower expressions MMP-2,-9 in severe preeclamptic placentas than in normal. MMP-2,-9 proteins were all present in each placental tissue. The expression for MMP-2,-9 was weaker in severe preeclamptic placenta than in normal. MMP-2,-9 were localized only to the trophoblast, and were also weakly positive in severe preeclamptic placenta compared with normal. Conclusions: MMP-2,-9 expressions were decreased in severe preeclampsia placenta compared to those from normal placenta. This study suggests that decreased expression of MMP-2,-9 may have a role in the development of severe preeclampsia.

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