인간만삭태반에서의 배아성 줄기세포표지자의 발현
Expression of embryonic stem cell markers in human term placenta
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목적: 만삭임신 태반에서의 배아성 줄기세포포지자의 부위별 발현을 비교하기 위하여 본 연구를 시행하였다. 연구 방법: 만삭임신의 태반에서 배아성 줄기세포 표면항원인 TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) 및 SSEA-4 (stage-specific embryonic antigen-4)에 대한 면역조직화학적 방법을 이용하여 태반의 부위별 항원 발현을 비교하였다. 결과: 양막상피세포는 TRA 1-60과 TRA 1-81에 대하여 양성반응을 보였고 양막중간엽기질세포는 TRA 1-81에 대하여 양성 반응을 보였다. 융모막중간엽기질세포는 TRA 1-60과 TRA 1-81에 대하여 양성반응을 보였다. SSEA-3와 SSEA-4는 태반의 어느 부위에서도 염색이 되지 않았다. TRA 1-60이 가장 강하게 발현된 부위는 양막상피세포였고 TRA 1-81이 가장 강하게 발현된 부위는 융모막중간엽기질세포였다. 결론: 만삭 태반에서의 양막상피세포 뿐 아니라 양막 및 융모막기질세포도 다능성 줄기세포의 유용한 자원이 될 수 있을 것으로 사료된다.
Abstract
Objective: To determine which compartments of placenta in the term pregnancy express the embryonic stem cell markers. Methods: We have used immunohistochemical methods with antibodies to embryonic stem cell surface antigens, TRA 1-60 (Tumor rejection antigen 1-60), TRA 1-81 (Tumor rejection antigen 1-81), SSEA-3 (stage-specific embryonic antigen-3) and SSEA-4 (stage-specific embryonic antigen-4), to identify and localize stem cells in the term placenta. Results: Stem cell marker-positive cells were found in all layer of placenta. Amnionic epithelial cells was immunoreactive with TRA 1-60, TRA 1-81. Amnionic mesenchymal stromal cells was immunoreactive with TRA 1-81. Chorionic mesenchymal stromal cells was immunoreactive with TRA 1-60 and TRA 1-81. SSEA-3 and SSEA-4 were not stained at any compartment of the term placenta. Compartment that was stained most strongly by TRA 1-60 was the amnionic epithelial cells layer. Compartment that was stained most strongly by TRA 1-81 was the chorionic mesenchymal stromal cells layer. Conclusion: The mesenchymal stroma cells of the amnion and chorion as well as amnionic epithelial cells may be useful source of pluripotent stem cells in the term placenta.
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