CaSki 세포주에 대한 비소화합물의 세포 성장 억제 및 세포자멸사
Inhibition of cell growth and apoptosis in CaSki, cervical cancer cell line by arsenic compounds
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목적: Paclitaxel, cisplatin, 삼산화비소, 육산화사비소를 단독 혹은 병합 투여하여 CaSki 자궁경부암 세포주에 대한 성장 억제와 세포자멸사를 비교해 보고자 하였다. 연구 방법: 약물 농도 및 시간에 따른 세포 성장 억제 정도를 각 약물을 투여한 후 water-soluble tetrazolium salts (WSTs) -1 assay로 측정하여 비교하였다. 각 약물을 단독 혹은 병합 투여한 후 세포를 annexin V/PI 염색한 후 그 정도를 흐름 유세포 분석기를 이용하여 세포자멸사 정도를 분석하였다. 결과: Paclitaxel, cisplatin, 삼산화비소, 육산화사비소는 농도와 처리 시간에 의존적으로 CaSki 세포성장을 억제하였다. 특히, 육산화사비소는 삼산화비소보다 세포성장을 더 많이 억제시켰다. Paclitaxel, cisplatin, 삼산화비소와 육산화사비소는 시간이 지남에 따라 세포자멸사를 진행시켰다. Cisplatin과 육산화사비소를 병용 투여한 군에서 다른 병용 투여군보다 세포자멸사가 더 많이 진행하였다. 결론: 육산화사비소는 CaSki 자궁경부암 세포주에 대해 다른 제제보다 더 강력한 항종양 효과를 나타내었다. 임상시험을 통해 육산화사비소의 항종양효과에 대한 더 많은 연구가 필요할 것으로 생각된다.
Abstract
Objective: To compare inhibition of cell growth and apoptosis in human cervical cancer cell lines (CaSki) by paclitaxel, cisplatin, arsenic trioxide and tetraarsenic oxide. Methods: Inhibition of cell growth was determined by the water-soluble tetrazolium salts (WSTs) -1 assay. For apoptosis analysis in CaSki cell line treated with single or combination of two agents, CaSki cell line treated with each agent was stained with annexin-V/PI and flow cytometry was performed. Results: Progression of apoptosis in CaSki cell line treated with paclitaxel, cisplatin, arsenic trioxide, and tetraarsenic oxide was time dependent. Inhibition of cell growth in CaSki cell line by paclitaxel, cisplatin, arsenic trioxide, and tetraarsenic oxide was dose and time dependent. Especially, tetraarsenic oxide was more effective in inhibition of CaSki cell growth compared to arsenic trioxide. Group treated with combination of cisplatin and tetraarsenic oxide showed more progressive apoptosis compared to other combination group. Conclusion: Tetraarsenic oxide has more potent anti-tumor effects than other agents on CaSki cell line. We need to consider further study about antitumor effect of tetraarsenic oxide through clinical study.
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