The expression of FAS-associated factor 1 and heat shock protein 70 in ovarian cancer
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
ObjectiveIn this study, we evaluated the expression of FAS-associated factor 1 (FAF1) and heat shock protein 70 (HSP70) in normal ovary and ovarian cancer, and also analyzed the correlation between FAF1 and HSP70 in ovarian cancer. MethodsThe patient group consisted of 29 unrelated Korean women diagnosed as ovarian cancers and control samples were obtained from 7 patients who underwent oophorectomy for benign disease of uterus, and normal ovary was confirmed histologically from biopsy. We examined FAF1 and HSP70 expression by western blot analysis and immunohistochemical staining in normal ovary and ovarian cancer. Furthermore, we examined a correlation between FAF1 and HSP70 in ovarian cancer. ResultsThe expression of FAF1 was lower in ovarian cancer than that in normal ovary (P=0.02), and the expression of HSP70 was increased in ovarian cancer in comparison to that in normal ovary (P=0.03). The expression of FAF1 was decreased in advanced stages (stage III or stage IV) as compared with early stages (stage I or stage II) (P=0.01). The expression of HSP70 was not significantly related with ovarian cancer histology (P=0.10), but the expression of HSP70 was most increased with papillary serous carcinomas and undifferentiated ovarian cancer. The expression of FAF1 was inversely correlated with the expression of HSP70 in ovarian cancer (Spearman correlation coefficience=-0.47). ConclusionWe concluded that the expression of FAF1 or HSP70 each seems to have a meaning as a biomarker for early detection of ovarian cancer. The expressions of FAF1 and HSP70 seem to be more valuable in predicting ovarian cancer when used together because of their inverse correlation. This is the first study about the expression of FAF1 in ovarian cancer and the correlation between FAF1 and HSP70 expression in ovarian cancer.
Abstract
ObjectiveIn this study, we evaluated the expression of FAS-associated factor 1 (FAF1) and heat shock protein 70 (HSP70) in normal ovary and ovarian cancer, and also analyzed the correlation between FAF1 and HSP70 in ovarian cancer. MethodsThe patient group consisted of 29 unrelated Korean women diagnosed as ovarian cancers and control samples were obtained from 7 patients who underwent oophorectomy for benign disease of uterus, and normal ovary was confirmed histologically from biopsy. We examined FAF1 and HSP70 expression by western blot analysis and immunohistochemical staining in normal ovary and ovarian cancer. Furthermore, we examined a correlation between FAF1 and HSP70 in ovarian cancer. ResultsThe expression of FAF1 was lower in ovarian cancer than that in normal ovary (P=0.02), and the expression of HSP70 was increased in ovarian cancer in comparison to that in normal ovary (P=0.03). The expression of FAF1 was decreased in advanced stages (stage III or stage IV) as compared with early stages (stage I or stage II) (P=0.01). The expression of HSP70 was not significantly related with ovarian cancer histology (P=0.10), but the expression of HSP70 was most increased with papillary serous carcinomas and undifferentiated ovarian cancer. The expression of FAF1 was inversely correlated with the expression of HSP70 in ovarian cancer (Spearman correlation coefficience=-0.47). ConclusionWe concluded that the expression of FAF1 or HSP70 each seems to have a meaning as a biomarker for early detection of ovarian cancer. The expressions of FAF1 and HSP70 seem to be more valuable in predicting ovarian cancer when used together because of their inverse correlation. This is the first study about the expression of FAF1 in ovarian cancer and the correlation between FAF1 and HSP70 expression in ovarian cancer.
참고문헌 (39)
- American Cancer Society, "Cancer facts & figures" (2010), American Cancer Society
- National Cancer Control Institute, "2008 National cancer statistics", National Cancer Center, ~ (2010)
- Ren J, "Tumor markers for early detection of ovarian cancer", Expert Rev Mol Diagn, 10, pp.787~798 (2010)
- Kabawat SE, "Tissue distribution of a coelomic-epitheliumrelated antigen recognized by the monoclonal antibody OC125", Int J Gynecol Pathol, 2, pp.275~285 (1983)
- Jacobs I, "The CA 125 tumour-associated antigen:a review of the literature", Hum Reprod, 4, pp.1~12 (1989)
- Berek JS, "Ovarian cancer screening. The use of serial complementary tumor markers to improve sensitivity and specificity for early detection", Cancer, 76(10 Suppl), pp.2092~2096 (1995)
- Gadducci A, "The concomitant determination of different tumor markers in patients with epithelial ovarian cancer and benign ovarian masses: relevance for differential diagnosis", Gynecol Oncol, 44, pp.147~154 (1992)
- Anderson GL, "Ovarian cancer biomarker screening:still too early to tell", Womens Health (Lond Engl), 6, pp.487~490 (2010)
- Yu JW, "FLIP and the death effector domain family", Oncogene, 27, pp.6216~6227 (2008)
- Chu K, "A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis", Proc Natl Acad Sci U S A, 92, pp.11894~11898 (1995)
- Ryu SW, "Apoptosis induced by human Fas-associated factor 1, hFAF1, requires its ubiquitin homologous domain, but not the Fas-binding domain", Biochem Biophys Res Commun, 286, pp.1027~1032 (2001)
- Menges CW, "FAS-associated factor 1 (FAF1): diverse functions and implications for oncogenesis", Cell Cycle, 8, pp.2528~2534 (2009)
- Bjorling-Poulsen M, "The pro-apoptotic FAS-associated factor 1 is specifically reduced in human gastric carcinomas", Int J Oncol, 23, pp.1015~1023 (2003)
- Hartl FU, "Molecular chaperones in cellular protein folding", Nature, 381, pp.571~579 (1996)
- Bukau B, "The Hsp70 and Hsp60 chaperone machines", Cell, 92, pp.351~366 (1998)
- Chuma M, "Expression profiling in multistage hepatocarcinogenesis:identification of HSP70 as a molecular marker of early hepatocellular carcinoma", Hepatology, 37, pp.198~207 (2003)
- Kim HJ, "Human Fasassociated factor 1 interacts with heat shock protein 70and negatively regulates chaperone activity", J Biol Chem, 280, pp.8125~8133 (2005)
- Bea S, "Uniparental disomies, homozygous deletions, amplifications, and target genes in mantle cell lymphoma revealed by integrative high-resolution whole-genome profiling", Blood, 113, pp.3059~3069 (2009)
- Weersma RK, "Confirmation of multiple Crohn’s disease susceptibility loci in a large Dutch-Belgian cohort", Am J Gastroenterol, 104, pp.630~638 (2009)
- Hidalgo A, "Microarray comparative genomic hybridization detection of chromosomal imbalances in uterine cervix carcinoma", BMC Cancer, 5, pp.77~ (2005)
- Suto R, "A mechanism for the specific immunogenicity of heat shock protein-chaperoned peptides", Science, 269, pp.1585~1588 (1995)
- Asea A, "HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine", Nat Med, 6, pp.435~442 (2000)
- Jaattela M, "Overexpression of major heat shock protein hsp70 inhibits tumor necrosis factor-induced activation of phospholipase A2", J Immunol, 151, pp.4286~4294 (1993)
- Soti C, "Molecular chaperones in the etiology and therapy of cancer", Pathol Oncol Res, 4, pp.316~321 (1998)
- Nylandsted J, "Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization", J Exp Med, 200, pp.425~435 (2004)
- Proskuryakov SY, "Necrosis:a specific form of programmed cell death?", Exp Cell Res, 283, pp.1~16 (2003)
- Kaur J, "Cell surface expression of 70 kDa heat shock protein in human oral dysplasia and squamous cell carcinoma: correlation with clinicopathological features", Oral Oncol, 34, pp.93~98 (1998)
- Trieb K, "Reversible downregulation of telomerase activity by hyperthermia in osteosarcoma cells", Int J Hyperthermia, 16, pp.445~448 (2000)
- Park MY, "Fas-associated factor-1 inhibits nuclear factor-kappaB (NF-kappaB)activity by interfering with nuclear translocation of the RelA (p65) subunit of NF-kappaB", J Biol Chem, 279, pp.2544~2549 (2004)
- McConkey DJ, "Mechanisms of proteasome inhibitor action and resistance in cancer", Drug Resist Updat, 11, pp.164~179 (2008)
- Baud V, "Is NF-kappaB a good target for cancer therapy? Hopes and pitfalls", Nat Rev Drug Discov, 8, pp.33~40 (2009)
- Fan XM, "Inhibition of proteasome function induced apoptosis in gastric cancer", Int J Cancer, 93, pp.481~488 (2001)
- Birle DC, "Suppression of the hypoxia-inducible factor-1 response in cervical carcinoma xenografts by proteasome inhibitors", Cancer Res, 67, pp.1735~1743 (2007)
- Yang DT, "Prevalence of bortezomib-resistant constitutive NFkappaB activity in mantle cell lymphoma", Mol Cancer, 7, pp.40~ (2008)
- Lazaris AC, "Proliferating cell nuclear antigen and heat shock protein 70 immunolocalization in invasive ductal breast cancer not otherwise specified", Breast Cancer Res Treat, 43 (1997)
- Athanassiadou P, "Expression of p53, bcl-2 and heat shock protein (hsp72) in malignant and benign ovarian tumours", Eur J Cancer Prev, 7, pp.225~231 (1998)
- Kim KK, "HSP70 and ER expression in cervical intraepithelial neoplasia and cervical cancer", J Korean Med Sci, 13, pp.383~388 (1998)
- Nanbu K, "Prognostic significance of heat shock proteins HSP70 and HSP90 in endometrial carcinomas", Cancer Detect Prev, 22, pp.549~555 (1998)
- Bonay M, "Expression of heat shock proteins in human lung and lung cancers", Am J Respir Cell Mol Biol, 10, pp.453~461 (1994)
