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氣虛, 血虛 動物模型의 一般 特性 및 皮膚 特性 硏究

A Study on the Skin Characteristics of Qi Deficiency and Blood Deficiency Animal Model

김윤범|신윤진
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2009.04|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제22권 제1호|pp. 76-88

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키워드

melanin indexQi DeficiencyBlood DeficiencyAnimal Modelerythema indextransepidermal water loss
주제분류한의학

초록

Back ground and Objective : There is a need for objectification and scientific verification of Pattern identification in Oriental medicine. The purpose of this study was to investigate the skin characteristics of Qi deficiency and Blood deficiency animal models. Material and Methods : Sprague-Dawley rats were divided into three groups: normal group, Qi deficiency group and Blood deficiency group. The Qi deficiency animal model was induced through restriction of food (75g/㎏/day) for 20 days. Blood deficiency animal model was induced by bleeding from tail vein(0.3 ㎖ /time) 8 times. The normal animal model was kept without any intervention. The general condition was observed by measuring body weight, body temperature, blood pressure, pulse rate, and hematological and biochemical parameters. The skin characteristics were observed by measuring the erythema index(EI), melanin index(MI), transepidermal water loss(TEWL) and dermal microcirculation. Results : 1. In the Qi deficiency group, body weight was lower than the other groups. In the Qi deficiency group, blood pressure was lower than in the Normal group. There was no difference in body temperature and pulse rate between the three groups. 2. In the Qi deficiency group, blood sugar was lower than in the Blood deficiency group. There was no difference in triacylglycerol between the three groups. In the Qi deficiency group, the WBC count was lower than in the Blood deficiency group. RBC count was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively. In the Qi deficiency group, Hb and Hct were higher than the other groups. 3. EI and MI were decreased in the Qi deficiency group, and EI showed a significant decrease. 4. EI and MI were increased in the Blood deficiency group, and MI showed a significant increase. 5. TEWL was significantly increased in the Qi deficiency group, while it was decreased in the Blood deficiency group. TEWL was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively and all three groups showed significant difference. 6. In the Qi deficiency group, dermal microcirculation was lower than the other groups. Conclusion : The above results show that the erythema index decreases in the Qi deficiency model, and the melanin index increases in the Blood deficiency model. The Qi deficiency animal model shows an increase in transepidermal water loss, while the Blood deficiency animal model shows a decrease. Further studies should develop new models of Pattern Identification that are more specific

Abstract

Back ground and Objective : There is a need for objectification and scientific verification of Pattern identification in Oriental medicine. The purpose of this study was to investigate the skin characteristics of Qi deficiency and Blood deficiency animal models. Material and Methods : Sprague-Dawley rats were divided into three groups: normal group, Qi deficiency group and Blood deficiency group. The Qi deficiency animal model was induced through restriction of food (75g/㎏/day) for 20 days. Blood deficiency animal model was induced by bleeding from tail vein(0.3 ㎖ /time) 8 times. The normal animal model was kept without any intervention. The general condition was observed by measuring body weight, body temperature, blood pressure, pulse rate, and hematological and biochemical parameters. The skin characteristics were observed by measuring the erythema index(EI), melanin index(MI), transepidermal water loss(TEWL) and dermal microcirculation. Results : 1. In the Qi deficiency group, body weight was lower than the other groups. In the Qi deficiency group, blood pressure was lower than in the Normal group. There was no difference in body temperature and pulse rate between the three groups. 2. In the Qi deficiency group, blood sugar was lower than in the Blood deficiency group. There was no difference in triacylglycerol between the three groups. In the Qi deficiency group, the WBC count was lower than in the Blood deficiency group. RBC count was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively. In the Qi deficiency group, Hb and Hct were higher than the other groups. 3. EI and MI were decreased in the Qi deficiency group, and EI showed a significant decrease. 4. EI and MI were increased in the Blood deficiency group, and MI showed a significant increase. 5. TEWL was significantly increased in the Qi deficiency group, while it was decreased in the Blood deficiency group. TEWL was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively and all three groups showed significant difference. 6. In the Qi deficiency group, dermal microcirculation was lower than the other groups. Conclusion : The above results show that the erythema index decreases in the Qi deficiency model, and the melanin index increases in the Blood deficiency model. The Qi deficiency animal model shows an increase in transepidermal water loss, while the Blood deficiency animal model shows a decrease. Further studies should develop new models of Pattern Identification that are more specific

참고문헌 (41)

  1. 李軍蘭, "氣虛證 動物模型 造模方法 綜述", 上海中醫藥大學學報, 18(3), pp.56~60 (2004)
  2. 王曉明, "“脾胃後天之本”的分子生物學基础", 遼寧中醫雜誌, 21(3), pp.139~141 (1994)
  3. HU Xin-gang, "Research on the External Signs and Hemorheology in Rats with Qi deficiency and blood stasis", Tianjin Journal of Traditional Chinese Medicine, 24(2), pp.138~141 (2007)
  4. 丘小惠, "不同炮制工艺制首烏對大鼠血虛模型的作用硏究", Journal of Chinese Medicinal Materials, 31(1), pp.14~17 (2008)
  5. 扈新刚, "Study on Exterior Signs and Related Biological Foundation of Qi-deficiency and Blood-stasis", 박사, pp.2007
  6. 焦立红, "四种血虚小鼠模型制备方法的比较", Lishizhen Medicine and Materia Medica Research, 17(6), pp.1001~1002 (2006)
  7. 吴捷, "养血妇康颗粒剂对实验性气血虚证动物的影响", Shaanxi Journal of Traditional Chinese Mediticne, 23(5), pp.467~469 (2002)
  8. 林海青, "西洋参 对大鼠“气虚”模型的实验研究", Chinese Archives of Traditional Chinese Medicine, 24(11), pp.2111~ (2006)
  9. Miao Ming-san, "Effect of fructus jujubae polysaccharide on hemogram indexes and activity of ATPase in erythrocyte of rats with blood deficiency", Chinese Journal of Clinical Rehabilitation, 10(11), pp.97~99 (2006)
  10. 謝萍, "産舒顆粒對氣虛血瘀血虛大鼠免疫作用的影響", 中國中醫 急症, 16(1), pp.76~78 (2007)
  11. 邱劍鋒, "養血潤腸方 對 小鼠 血虛型 慢性功能性便秘 結腸組織勻漿及血淸中NO的 影響", 江西中醫學院學報, 19(3), pp.68~69 (2007)
  12. Wang Hui, "Influence of Astragalus combined with Angelica on expressionof CD54 in endothelial cells of vessels and hepatic sinusoid in Qi-Deficiency and Blood- Stasis(气虚血瘀) Model rats", Pharmacology and Clinics of Chinese Materia Medica, (5), pp.65~70 (2007)
  13. 彭欣, "物汤对血虚模型大鼠血流变和凝血功能的影响", 山东中医药大学学报, (2), pp.106~110 (2008)
  14. Miao Mingsan, "Effects of Gross Polysaccharide of Radix Rehmanniae Preparata on Morphology Changes of Thymus and Spleen in Blood Deficiency Model Mice", China Journal of Traditional Chinese Medicine and Pharmacy, (5), pp.373~381 (2007)
  15. 李星, "健脾补肾汤对血虚模型大鼠T细胞分泌IL-2的影响", 福建中医学院学报, (1), pp.40~43 (2008)
  16. 김성수, "Hydrocortisone acetate로 유발된 陽虛 動物模型에 관한 연구", 대한한의학회지, 7(2), pp.103~106 (1986)
  17. 강성길, "水蔘, 白蔘 및 紅蔘製劑가 人蔘水鍼刺戟의 陽虛 動物模型에 미치는 영향", 대한한의학회지, 10(1), pp.28~41 (1989)
  18. 안규석, "瘀血病態模型 개발에 관한 연구", 대한동의병리학회지, 13(1), pp.25~35 (1999)
  19. 조남인, "丹蔘飮과 그 구성약물이 瘀血病態模型에 미치는 영향", 대한한의학회지, 12(2), pp.66~77 (1991)
  20. 강순희, "淸胞逐瘀湯 및 蜂毒이 瘀血病態模型에 미치는 영향", 대한한의학회지, 16(1), pp.351~369 (1995)
  21. 김성수, "Hydrocortisone acetate로 유발된 瘀血病態模型에 관한 연구", 대한한의학회지, 8(1), pp.133~138 (1987)
  22. 양문혁, "疎風活血湯 및 疎風活血湯加蜂毒이 瘀血病態에 미치는 영향", 대한한의학회지, 15(1), pp.385~399 (1994)
  23. Takushi X, "An Analysis of Blood Pressure Effects of Nipradilol and Prizidilol in Normotensive and Spontaneously Hypertensive Rats", Japan J Pharmacol, 38, pp.273~279 (1985)
  24. 장동덕, "채혈방법에 따른 혈액상 변화 연구", 한국수의공중보건학회지, 19(1), pp.75~80 (1995)
  25. 강수경, "얼굴부위의 홍반 및 멜라닌지수와 일반적 특성과의 관련성", 한국미용학회지, 13(2), pp.507~513 (2007), 한국미용학회
  26. Chang Shik Yin, "proposed transpositional acupoint system in a mouse and rat model", Research in veterinary science, 84(2), pp.159~165 (2008)
  27. 차재훈, "20-30대 한국인의 경피수분 손실도 연구", 한방안이비인후피부과학회지, 20(2), pp.179~186 (2007), 대한한방안이비인후피부과학회
  28. 급성 피부장벽파괴 동물모델에 대한 스트론튬의 피부보습효과, "민대기", 경희대학교 대학원, 2~ (2007)
  29. Stanislav Sarnika, "LASER DOPPLER FLUXMETRY", Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 151(1), pp.143~146 (2007)
  30. 정우열, "血虛病證의 지표설정에 대한 이론적 연구", 대한동의병리학회지, 3, pp.5~8 (1988)
  31. 김완희, "韓醫學原論", pp.121~128 (1995), 성보사
  32. 이승헌, "피부장벽", pp.103~105 (2004), 도서출판 여문각
  33. Tagamo H, "Interrelationship between water barrier and reservoir function in pathologic stratum corneum", Arch Dermatol, 121, pp.642~645 (1985)
  34. Bouwstra JA, "The skin barrier in healthy and disease state", Biochim BioPhys Acta, 1758(12), pp.2080~2095 (2006)
  35. 민복기, "피부 표면 온도의 변화와 diffusion chamber 내의 온도 변화에 따른 경표피수분손실 및 피부장벽 회복률의 변동", 대한피부과학회지, 34(6), pp.875~885 (1996)
  36. Hsin Hsiu, "Microcirculatory effect of different skin contacting pressures around the blood pressure", Physiol Meas, 29, pp.1421~1434 (2008)
  37. M. Rossi, "Skin vasomotion investigation: A useful tool for clinical evaluation of microvascular endothelial function?", Biomedicine & Pharmacotherapy, 62, pp.541~545 (2008)
  38. 김성진, "한국인에서 피부생물공학적 측정기법을 이용한 피부자극반응의 표준화에 대한 연구", 대한피부과학회지, 41(11), pp.1440~1447 (2003), 대한피부과학회
  39. 정우열, "韓方病理學", pp.34~ (1988), 원광대학교 한의과대학병리학교실
  40. 裵秉哲, "(國譯)黃帝內經: 素問ㆍ靈樞", pp.160~641 (2000), 成輔社
  41. 許浚, "(對譯)東醫寶鑑", pp.115~261 (2002), 法仁文化社

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