Sequential Changes in Aberrant Crypt Foci and Lectin Expression in the Early and Late Stages of DMH-Induced Colon Carcinogenesis in Rats
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background/Aims: The purpose of this study was to investigate the malignant potential of aberrant crypt foci (ACF) by measuring the multiplicity of crypts and lectin expression in the early and late stages of 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Methods: Six-week-old Wistar rats were injected subcutaneously with DMH for 27 weeks. We classified ACF according to the number of crypts per ACF as a few crypts (≤3 crypts, FC ACF) or numerous crypts (≥4 crypts, NC ACF). Immunohistochemistry was used to evaluate lectin expression. Results: In the early stage, FC ACF (590/1,902, 31.0%) occurred more frequently than NC ACF (35/449, 7.8%); whereas in the late stage, NC ACF (176/449, 39.2%) occurred more frequently than FC ACF (324/1,902, 17.0%). The number of ACF peaked at 15 to 20weeks. The ratio of NC/FC ACF increased gradually during carcinogenesis. The expression of both UEA1 and PNA was higher in NC ACF than FC ACF. Lectin expression increased in the late stage compared with the early stage. Conclusions:The expression of lectin was higher in NC ACF and ACF in the late stage. Therefore, ACF with higher multiplicities in the late stage may have more malignant potential in DMH-induced colon carcinogenesis.
Abstract
Background/Aims: The purpose of this study was to investigate the malignant potential of aberrant crypt foci (ACF) by measuring the multiplicity of crypts and lectin expression in the early and late stages of 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Methods: Six-week-old Wistar rats were injected subcutaneously with DMH for 27 weeks. We classified ACF according to the number of crypts per ACF as a few crypts (≤3 crypts, FC ACF) or numerous crypts (≥4 crypts, NC ACF). Immunohistochemistry was used to evaluate lectin expression. Results: In the early stage, FC ACF (590/1,902, 31.0%) occurred more frequently than NC ACF (35/449, 7.8%); whereas in the late stage, NC ACF (176/449, 39.2%) occurred more frequently than FC ACF (324/1,902, 17.0%). The number of ACF peaked at 15 to 20weeks. The ratio of NC/FC ACF increased gradually during carcinogenesis. The expression of both UEA1 and PNA was higher in NC ACF than FC ACF. Lectin expression increased in the late stage compared with the early stage. Conclusions:The expression of lectin was higher in NC ACF and ACF in the late stage. Therefore, ACF with higher multiplicities in the late stage may have more malignant potential in DMH-induced colon carcinogenesis.
참고문헌 (22)
- Gupta AK, "Aberrant crypt foci: are they intermediate endpoints of colon carcinogenesis in humans?", Curr Opin Gastroenterol, 25, pp.59~65 (2009)
- Raju J, "Azoxymethane-induced rat aberrant crypt foci: relevance in studying chemoprevention of colon cancer", World J Gastroenterol, 14, pp.6632~6635 (2008)
- Alrawi SJ, "Aberrant crypt foci", Anticancer Res, 26(1A), pp.107~119 (2006)
- Bird RP, "Role of aberrant crypt foci in understanding the pathogenesis of colon cancer", Cancer Lett, 93, pp.55~71 (1995)
- Papanikolaou A, "Sequential and morphological analyses of aberrant crypt foci formation in mice of differing susceptibility to azoxymethaneinduced colon carcinogenesis", Carcinogenesis, 21, pp.1567~1572 (2000)
- Ghirardi M, "Azoxymethane-induced aberrant crypt foci and colorectal tumors in F344 rats: sequential analysis of growth", Eur Surg Res, 31, pp.272~280 (1999)
- Yamada Y, "Sequential analysis of morphological and biological properties of beta-catenin-accumulated crypts, provable premalignant lesions independent of aberrant crypt foci in rat colon carcinogenesis", Cancer Res, 61, pp.1874~1878 (2001)
- Maurin N, "Progression of tumors arising from large ACF is associated with the MUC5AC expression during rat colon MNNG carcinogenis", Int J Cancer, 120, pp.477~483 (2007)
- Orlando FA, "Aberrant crypt foci as precursors in colorectal cancer progression", J Surg Oncol, 98, pp.207~213 (2008)
- Bird RP, "The significance of aberrant crypt foci in understanding the pathogenesis of colon cancer", Toxicol Lett, 112, pp.395~402 (2000)
- Rüdiger H, "Plant lectins: occurrence, biochemistry, functions and applications", Glycoconj J, 18, pp.589~613 (2001)
- Thom I, "Lectin histochemistry of metastatic adenocarcinomas of the lung", Lung Cancer, 56, pp.391~397 (2007)
- Perše M, "Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats", J Biomed Biotechnol, 2011, pp.473964~ (2011)
- Caderni G, "Characterisation of aberrant crypt foci in carcinogen-treated rats: association with intestinal carcinogenesis", Br J Cancer, 71, pp.763~769 (1995)
- Khare S, "Aberrant crypt foci in colon cancer epidemiology", Methods Mol Biol, 472, pp.373~386 (2009)
- Figueiredo P, "Aberrant crypt foci: endoscopic assessment and cell kinetics characterization", Int J Colorectal Dis, 24, pp.441~450 (2009)
- Liu FT, "Galectins as modulators of tumour progression", Nat Rev Cancer, 5, pp.29~41 (2005)
- Thies A, "Lectin binding to cutaneous malignant melanoma: HPA is associated with metastasis formation", Br J Cancer, 84, pp.819~823 (2001)
- Blonski K, "Ulex europeus agglutinin-I binding as a potential prognostic marker in ovarian cancer", Anticancer Res, 27(4C), pp.2785~2790 (2007)
- Erhan Y, "Ulex Europeus Agglutinin (UEA-I) lectin binding in breast carcinoma and its relationship to prognostic factors", Ann Saudi Med, 16, pp.509~511 (1996)
- Kannan S, "Expression of peanut agglutinin-binding mucin-type glycoprotein in human esophageal squamous cell carcinoma as a marker", Mol Cancer, 2, pp.38~ (2003)
- Suzuki H, "Expression of peanut agglutinin-binding carbohydrates correlates with nodal involvement in human lung adenocarcinoma", Cancer Lett, 187, pp.215~221 (2002)