CD64 Expression Is Increased in Patients with Severe Acute Pancreatitis: Clinical Significance
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Background/Aims: Upregulated CD64 expression on neutrophilsis the most useful marker for acute bacterial infectionsand systemic inflammation. However, it is unknownwhether CD64 is involved in the pathogenesis of acute pancreatitis(AP). This study was designed to determine whetherCD64 is implicated in severe acute pancreatitis (SAP), andthus, is a suitable marker for SAP. Methods: SAP was inducedin rats with an intraperitoneal injection of L-arginine. CD64 expression in the rat pancreas was determined byquantitative real-time polymerase chain reaction (qRT-PCR)and immunohistochemistry. Additionally, the CD64 mRNAexpression in peripheral blood leukocytes from 21 patientswith mild acute pancreatitis (MAP) and 10 patients with SAPwas investigated at the time of admission and during remissionby qRT-PCR. Results: CD64 mRNA and protein expressionin the pancreas was significantly higher in rats with SAP,compared to the controls. The CD64 expression was higherin the patients with SAP than in the patients with MAP. Duringremission, CD64 mRNA decreased in both the MAP andSAP patients. The area under the curve of CD64 expressionfor the detection of SAP was superior to both the Ranson andthe Acute Physiology and Chronic Health Evaluation II scores. Conclusions: The CD64 level was significantly increased incorrelation with the disease severity in SAP and may act asa useful marker for predicting the development of SAP.
Abstract
Background/Aims: Upregulated CD64 expression on neutrophilsis the most useful marker for acute bacterial infectionsand systemic inflammation. However, it is unknownwhether CD64 is involved in the pathogenesis of acute pancreatitis(AP). This study was designed to determine whetherCD64 is implicated in severe acute pancreatitis (SAP), andthus, is a suitable marker for SAP. Methods: SAP was inducedin rats with an intraperitoneal injection of L-arginine. CD64 expression in the rat pancreas was determined byquantitative real-time polymerase chain reaction (qRT-PCR)and immunohistochemistry. Additionally, the CD64 mRNAexpression in peripheral blood leukocytes from 21 patientswith mild acute pancreatitis (MAP) and 10 patients with SAPwas investigated at the time of admission and during remissionby qRT-PCR. Results: CD64 mRNA and protein expressionin the pancreas was significantly higher in rats with SAP,compared to the controls. The CD64 expression was higherin the patients with SAP than in the patients with MAP. Duringremission, CD64 mRNA decreased in both the MAP andSAP patients. The area under the curve of CD64 expressionfor the detection of SAP was superior to both the Ranson andthe Acute Physiology and Chronic Health Evaluation II scores. Conclusions: The CD64 level was significantly increased incorrelation with the disease severity in SAP and may act asa useful marker for predicting the development of SAP.
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