The Effect of Intragastric Administration of Botulinum Toxin Type A on Reducing Adiposity in a Rat Model of Obesity Using Micro-CT and Histological Examinations
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Background/Aims: Botulinum toxin type A (BTX), a longacting inhibitor of muscular contraction in both striated and smooth muscles, is responsible for gastric motility. The aim of this study was to investigate the effects of an endoscopic intragastric BTX injection on weight loss, body fat accumulation, and gastric emptying time. Methods: The BTX group consisted of 15 obese rats in which 20 U of BTX were injected into the gastric antrum. The saline group consisted of 15 obese rats injected with 20 U of saline, and the control group included 10 obese rats that did not receive a surgical intervention. The gastric emptying time, biochemical parameters, and body fat volume were evaluated using microcomputed tomography (micro-CT) and histologic evaluations. Results: The postoperative body weight of the BTX group was significantly lower than those of the other groups (p<0.001) at 6 weeks after the operation. The gastric emptying time (156±54 minutes) was significantly delayed in the BTX group. The BTX group showed significantly lower lipid levels than the other groups. A reduction in body fat volume was observed in the BTX group using micro-CT and histological evaluations. Conclusions: BTX application to the gastric antrum represents a potentially effective treatment for obesity and may help improve the lipid profile by increasing the gastric emptying time.
Abstract
Background/Aims: Botulinum toxin type A (BTX), a longacting inhibitor of muscular contraction in both striated and smooth muscles, is responsible for gastric motility. The aim of this study was to investigate the effects of an endoscopic intragastric BTX injection on weight loss, body fat accumulation, and gastric emptying time. Methods: The BTX group consisted of 15 obese rats in which 20 U of BTX were injected into the gastric antrum. The saline group consisted of 15 obese rats injected with 20 U of saline, and the control group included 10 obese rats that did not receive a surgical intervention. The gastric emptying time, biochemical parameters, and body fat volume were evaluated using microcomputed tomography (micro-CT) and histologic evaluations. Results: The postoperative body weight of the BTX group was significantly lower than those of the other groups (p<0.001) at 6 weeks after the operation. The gastric emptying time (156±54 minutes) was significantly delayed in the BTX group. The BTX group showed significantly lower lipid levels than the other groups. A reduction in body fat volume was observed in the BTX group using micro-CT and histological evaluations. Conclusions: BTX application to the gastric antrum represents a potentially effective treatment for obesity and may help improve the lipid profile by increasing the gastric emptying time.
참고문헌 (24)
- Wang YC, "Health and economic burden of the projected obesity trends in the USA and the UK", Lancet, 378, pp.815~825 (2011)
- Swinburn BA, "The global obesity pandemic: shaped by global drivers and local environments", Lancet, 378, pp.804~814 (2011)
- Bays HE, "Current and investigational antiobesity agents and obesity therapeutic treatment targets", Obes Res, 12, pp.1197~1211 (2004)
- Mun EC, "Current status of medical and surgical therapy for obesity", Gastroenterology, 120, pp.669~681 (2001)
- Foschi D, "Plasma cholecystokinin levels after vertical banded gastroplasty: effects of an acidified meal", Obes Surg, 14, pp.644~647 (2004)
- Cummings DE, "Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery", N Engl J Med, 346, pp.1623~1630 (2002)
- Espinet-Coll E, "Current endoscopic techniques in the treatment of obesity", Rev Esp Enferm Dig, 104, pp.72~87 (2012)
- Albani G, "Safety and efficacy of therapy with botulinum toxin in obesity: a pilot study", J Gastroenterol, 40, pp.833~835 (2005)
- Gui D, "Effect of botulinum toxin antral injection on gastric emptying and weight reduction in obese patients: a pilot study", Aliment Pharmacol Ther, 23, pp.675~680 (2006)
- Coskun H, "Effect on gastric emptying and weight reduction of botulinum toxin-A injection into the gastric antral layer: an experimental study in the obese rat model", Obes Surg, 15, pp.1137~1143 (2005)
- Júnior AC, "Botulinum A toxin injected into the gastric wall for the treatment of class III obesity: a pilot study", Obes Surg, 16, pp.335~343 (2006)
- Kim CS, "Electrophysiological study for comparing the effect of biological activity between type A botulinum toxins in rat gastrocnemius muscle", Hum Exp Toxicol, 32, pp.914~920 (2013)
- Nam HS, "Efficacy and safety of NABOTA in post-stroke upper limb spasticity: a phase 3 multicenter, double-blinded, randomized controlled trial", J Neurol Sci, 357, pp.192~197 (2015)
- Won CH, "Comparative trial of a novel botulinum neurotoxin type A versus onabotulinumtoxinA in the treatment of glabellar lines: a multicenter, randomized, double-blind, active-controlled study", Int J Dermatol, 54, pp.227~234 (2015)
- Canny J, "A computational approach to edge detection", IEEE Trans Pattern Anal Mach Intell, 8, pp.679~698 (1986)
- Abraham S, "Do the digestive tract symptoms in eating disorder patients represent functional gastrointestinal disorders", BMC Gastroenterol, 13, pp.38~ (2013)
- Xing J, "Alterations of gastrointestinal motility in obesity", Obes Res, 12, pp.1723~1732 (2004)
- Cardoso-Júnior A, "Gastric emptying of solids and semi-solids in morbidly obese and non-obese subjects: an assessment using the 13C-octanoic acid and 13C-acetic acid breath tests", Obes Surg, 17, pp.236~241 (2007)
- Cigaina V, "Long-term follow-up of gastric stimulation for obesity: the Mestre 8-year experience", Obes Surg, 14(Suppl 1), pp.S14~S22 (2004)
- Chevallier JM, "Complications after laparoscopic adjustable gastric banding for morbid obesity: experience with 1,000 patients over 7 years", Obes Surg, 14, pp.407~414 (2004)
- James AN, "Inhibitory effects of botulinum toxin on pyloric and antral smooth muscle", Am J Physiol Gastrointest Liver Physiol, 285, pp.G291~G297 (2003)
- Adams LA, "The histological course of nonalcoholic fatty liver disease: a longitudinal study of 103 patients with sequential liver biopsies", J Hepatol, 42, pp.132~138 (2005)
- Donnelly KL, "Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease", J Clin Invest, 115, pp.1343~1351 (2005)
- Browning JD, "Short-term weight loss and hepatic triglyceride reduction: evidence of a metabolic advantage with dietary carbohydrate restriction", Am J Clin Nutr, 93, pp.1048~1052 (2011)