본문 바로가기
KERD
논문KCI 등재

인체 흑색종 세포에 대한 와송 추출물의 세포주기 억제를 통한 항암효과와 기전 연구

Anticancer and Signaling Mechanisms of Biologically Active Substances from Orostachys japonicus through Arrest of Cell cycle in Human Melanoma Cells

류덕현|류덕선
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2019.11|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제32권 제4호|pp. 1-12|DOI http://dx.doi.org/10.6114/jkood.2019.32.4.001

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

AnticancerMelanomaOrostachys JaponicusCell Cycle Arrest
주제분류한의학

초록

Objectives : The purpose of this study was to identify the anticancer effect of biological substances of ethylacetate(EtOAc) fraction from Orostachys japonicus(OJEF), their effect on human melanoma A375 cells and the related molecular mechanisms. Methods : The MTS assay was used to confirm the inhibition of cancer cell proliferation in A375 cells. And the MUSE™ analyzer was used to determine the ability of OJEF to induce cell cycle arrest. Western blotting was used to determine the changes in protein expression in A375 cells after treatment with OJEF. Results : OJEF showed cytotoxicity to A375 cells. And cell cycle arrest occurred in G1 phase and G2/M phase owing to inhibition of CDK1, cyclin B1, CDK4, and cyclin D, which are related to cell cycle regulation and cell division control. Conclusion : OJEF is effective in regulating cell cycle of human melanoma cells and thus can be a good theraputic agent to treat patients with melanoma.

Abstract

Objectives : The purpose of this study was to identify the anticancer effect of biological substances of ethylacetate(EtOAc) fraction from Orostachys japonicus(OJEF), their effect on human melanoma A375 cells and the related molecular mechanisms. Methods : The MTS assay was used to confirm the inhibition of cancer cell proliferation in A375 cells. And the MUSE™ analyzer was used to determine the ability of OJEF to induce cell cycle arrest. Western blotting was used to determine the changes in protein expression in A375 cells after treatment with OJEF. Results : OJEF showed cytotoxicity to A375 cells. And cell cycle arrest occurred in G1 phase and G2/M phase owing to inhibition of CDK1, cyclin B1, CDK4, and cyclin D, which are related to cell cycle regulation and cell division control. Conclusion : OJEF is effective in regulating cell cycle of human melanoma cells and thus can be a good theraputic agent to treat patients with melanoma.

참고문헌 (36)

  1. Sekulic A, "Malignant melanoma in the 21st century : the emerging molecular landscape", Mayo Clinic Proc, 83(7), pp.825~846 (2008)
  2. Rigel DS, "Malignant melanoma : prevention, early detection, and treatment in the 21st century", CA Cancer J Clin, 50(4), pp.215~236 (2000)
  3. Cho HK, "Molecular Mechanism of the Antiproliferative Effect by Ginseng Panaxynol on a Human Malignant Melanoma Cell Line, SK-MEL-1", J Ginseng Res, 23(3), pp.190~197 (1999)
  4. 권지혜, "인체 폐암 세포에 대한 와송 유래 에틸아세테이트 분획 생리 활성 물질의 세포사멸 유도 및 세포주기 억제 항암활성", 예술인문사회 융합 멀티미디어 논문지, 7(1), pp.395~405 (2017), 사단법인 인문사회과학기술융합학회[DOI]
  5. 이봉무, "안면부에 발생한 기저세포암 137례의 임상적 고찰", Archives of Craniofacial Surgery, 14(2), pp.107~110 (2013), 대한두개안면성형외과학회[DOI]
  6. Hussein MR, "Apoptosis and melanoma : molecular mechanisms", J Pathol, 199(3), pp.275~288 (2003)
  7. Helmbach H, "Drug-resistance in human melanoma", Int J Cancer, 93(5), pp.617~622 (2001)
  8. 강여주, "피부 멜라닌세포질환에서 악성 진행에 따른 세포자멸사 관련 단백들(Apaf-1, Caspase-9, Bcl-2, p53, and Survivin)의 변화", Journal of Pathology and Translational Medicine, 45(3), pp.247~253 (2011), 대한병리학회
  9. Yamamoto T, "Illustration map of cell signaling" (2006), World science
  10. Athar M, "Multiple molecular Targets of Resveratrol : Anti-carcinogenic Mechanisms", Arch Biochem Biophys, 486(2), pp.95~102 (2009)
  11. Ryu DS, "Orostachys japonicus induces apoptosis and cell cycle arrest through the mitochondria-dependent apoptotic pathway in AGS human gastric cancer cells", Int J Oncol, 45(1), pp.459~469 (2014)
  12. Jo CS, "A Literature Review about Orostachys japonicus", J Korean Med Daejeon Univ, 2(2), pp.83~98 (1993)
  13. Jae-Han Jeong, "Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPS-stimulated RAW 264.7 cells", BMB Reports, 44(6), pp.399~404 (2011), 생화학분자생물학회
  14. Je Ma C, "Calpain inhibitory flavonoids isolated from Orostacys japonicus", J Enzyme Inhib Medic Chem, 24(3), pp.676~679 (2009)
  15. Ryu DS, "Effects of the Ethylacetate Extract of Orostachys japonicus on Induction of Apoptosis through the p53-Mediated signaling Pathway in Human Gastric Cancer Cells", Biol Pharm Bull, 35(5), pp.660~665 (2012)
  16. 윤소영, "와송 부위별 추출물의 항균활성", 한국식품영양과학회지, 38(1), pp.14~18 (2009), 한국식품영양과학회
  17. 이수정, "Hypolipidemic and hypoglycemic effects of Orostachys japonicus A. Berger extracts in streptozotocin-induced diabetic rats", Nutrition Research and Practice, 5(4), pp.301~307 (2011), 한국영양학회
  18. 이경선, "Anti-cancer Activity of the Ethylacetate Fraction from Orostachys japonicus for Modulation of the Signaling Pathway in HepG2 Human Hepatoma Cells", Food Science and Biotechnology, 23(1), pp.269~275 (2014), 한국식품과학회[DOI]
  19. 류덕선, "Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells", BMB Reports, 43(11), pp.750~755 (2010), 생화학분자생물학회
  20. Kim CM, "Dictionary of Chinese herbs(7)", pp.4042~4044 (1998), Jungdam
  21. Lee WS, "Flavonoids from Orostachys Japonicus A. Berger Induces Caspase-dependent Apoptosis at Least Partly through Activation of p38 MAPK Pathway in U937 Human Leukemic Cells", Asian Pac J Cancer Prev, 16(2), pp.465~469 (2015)
  22. Park HJ, "Triterpene and Steroids from Orostachys japonicus", Kor J Pharmacog, 25(1), pp.20~23 (1994)
  23. 김진성, "와송의 수종 암세포에 대한 항암작용 연구", 대한한방내과학회지, 26(2), pp.333~340 (2005), 대한한방내과학회
  24. 김재용, "인체 대장암 세포주 SW480에서 재배 와송의 세포 사멸 유도 효과", 한국식품과학회지, 44(3), pp.317~323 (2012), 한국식품과학회[DOI]
  25. 원영선, "인체 전립선 암세포에서 재배 와송의 항암효과", 한국식품영양과학회지, 43(1), pp.67~73 (2014), 한국식품영양과학회
  26. 김경순, "귀전우, 백화사설초, 와송 추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화", 대한한방내과학회지, 32(1), pp.33~42 (2011), 대한한방내과학회
  27. 윤경수, "瓦松이 만성 골수성 백혈병 세포주(K562)에서 세포사멸에 미치는 영향", 대한한방내과학회지, 27(1), pp.166~177 (2006), 대한한방내과학회
  28. Oh HS, "Effects of Partial Cervus elaphus Linne’ Extract on Antitumoral Immune Response in Melanoma-induced Mice", J Korean Oriental Med, 14(1), pp.39~77 (2000)
  29. Elwood JM, "Melanoma and sun exposure", Semin Oncol, 23(6), pp.650~666 (1996)
  30. Garbe C, "Diagnosis and treatment of cutaneous melanoma : state of the art 2006", Melanoma Res, 17(2), pp.117~127 (2007)
  31. Pines J, "Protein kinases and cell cycle control", Semin Cell Biol, 5(6), pp.399~408 (1994)
  32. Elledge SJ, "Cdk inhibitors : on the threshold of checkpoints and development", Curr Opin Cell Biol, 6(6), pp.847~852 (1994)
  33. Blagosklonny MV, "The Restriction Point of the Cell Cycle", Cell cycle, 1(2), pp.102~109 (2002)
  34. Stark GR, "Analyzing the G2", M Checkpoint, 2004, pp.280(1), 51~82
  35. Vermeulen K, "The cell cycle : a review of regulation, deregulation and therapeutic targets in cancer", Cell Prolif, 36(3), pp.131~149 (2003)
  36. Ko HS, "Anticancer Effects of Gleditsin in Human Mammary Cancer Cells", Yeungnam Univ J Med, 24(2), pp.580~590 (2007)

관련 논문AI추천

瓦松이 HepG2 cell의 세포분열 및 관련유전자 발현에 미치는 영향김영철, 문영훈, 우홍정, 이장훈대한한방내과학회 · 대한한방내과학회지2005 · 제26권 제1호Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells김은영, 류덕선, 이동석, Geum-Ok Baek, Ki-Hoon Kim생화학분자생물학회 · BMB Reports2010 · 제43권 제11호Ethanol extract of Innotus obliquus (Chaga mushroom) induces G1 cell cycle arrest in HT-29 human colon cancer cells김선효, 김은지, 이현숙한국영양학회 · Nutrition Research and Practice2015 · 제9권 제2호溫淸飮의 조성 용량변화가 Hep3B 세포의 G1 arrest 기전에 미치는 영향구인모, 김길훤, 신흥묵한의병리학회 · 동의생리병리학회지2008 · 제22권 제5호土茯笭이 피부암 세포의 성장에 미치는 영향박수연, 송시열, 정민영, 최정화대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2024 · 제37권 제1호Orostachys japonicus induce caspase-dependent apoptosis in HeLa human cervical cancer cells김선희, 이동석한국영양학회 · Nutrition Research and Practice2025 · 제19권 제1호

이 간행물의 다른 논문 (13건)

원형 탈모 환자 222명의 한의학적 치료 효과에 대한 후향적 연구이건석, 정보윤, 최진우, 이정환, 지유진pp. 13-28寒熱 변증에 따른 한의 외용 치료제 비염고의 치료 효과 : 하위 그룹 분석이동효, 손미주pp. 29-40임상연구방법론에서 귀무가설과 대립가설, 귀류법에 대한 고찰과 한방이비인후과에서 베이지안 통계학의 활용배재민, 권강, 남승표pp. 41-61알레르기성 비염에 대한 한약 및 양약 국소비강외용제의 효과 비교 : 체계적 문헌 고찰김선혜, 홍승욱, 김은정, 오세희, 조효림, 성원석pp. 62-89탈모에 대한 레이저의 안전성 및 유효성 : 체계적 문헌 고찰서형식, 고경숙, 이보람, 이마음pp. 90-100
원문 보기