辛夷淸肺飮이 알레르기 鼻炎에 미치는 效果에 대한 實驗的 硏究

Abstract

Objectives: It has a growing interest in the prevention and medical treatment of allergic rhinitis. According to many studies, it’s known that Sinicheongpye-um has the inhibitory effect on the allergic rhinitis. We have studied effect of the mice on OVA-induced Production of IL-4, IL-5, Interferone-γ by Murine Splenocytes and effect of OVA-induced Total IgE. Methods: Mixing Ovalbumin(OVA) 10㎍ into PBS(phoshate buffered saline) and Al(OH)3 gel solution and changing into 1㎖, we made it into OVA solution. That was administered to normal group. After the last administration into abdominal cavity, we caused allergic rhinitis in nasal cavity of mouse of control group and sample group administering 0.1% solution dropwise 3 times a day for 7 days. Keeping separated serum at -20 degree and after refloating spleen cells, cultivating the cells and centrifuging the upper liquid and keeping it at -20 degree, we measured the amount of IL-4, IL-5, Interferone-γ and OVA-induced Total IgE by ELISA. Results: 1. In Total IgE, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.05) 2. In IL-4 study, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.005) 3. In IL-5 study, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.001) 4. In Interferone-γ study, Sinichengpae-um treated group showed a increasing tendency. Conclusion: Based on the above result, it is considered that Sinichengpae-um has the inhibitory effect on the allergic rhinitis of mice and suggested that it could be uesd in relieving patients of the symptoms which are caused by allergic rhinitis.