加味玉液湯이 알레르기 반응에 미치는 영향
Effect of Gamiokyaek-tang on Allergic Reaction
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Abstract
Object: We investigated the effect of Gamiokyaek-tang(GOYT) on immediate type hypersensitivity. Methods: We investigated anti-dinitrophenyl(DNP) IgE-mediated passive cutaneous anaphylaxis(PCA) and acetic acid-induced vascular permeability in rodents. Also we measured MTT assay, β-hexosaminidase activity and IL-4 from RBL-2H3 and nitric oxide from Raw264.7. Results: GOYT inhibited passive cutaneous anaphylaxis and acetic acid-induced vascular permeability by oral administration. All the concentrations of GOYT from 0.1 to 5mg/ml didn't have an effect on cell viability and cytotoxicity. In RBL-2H3, β-hexosaminidase release and IL-4 production were significantly reduced by 1, 2 and 5mg/ml of GOYT. In Raw264.7, nitric oxide level was decreased by 5mg/ml of GOYT. Conclusion: These results indicate that GOYT have inhibition effects on immediate type hypersensitivity.
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