택사(Alismatis Rhizoma) 추출물의 반복 경구투여 독성 연구
Repeated Dose Oral Toxicity Study of Alismatis Rhizoma in SD Rats
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Objectives : This study was carried out to evaluate the repeated dose oral toxicity of Alismatis Rhizoma inSprague-Dawley(SD) rats. Methods : Male and female rats were administered orally with Alismatis Rhizoma water extract of 500 ㎎/㎏(lowdosage group), 1,000 ㎎/㎏(middle dosage group) and 2,000 ㎎/㎏(high dosage group). We daily observed numberof deaths, clinical signs and gross findings for 14 days(twice a day). After 14 days, we measured body and organsweight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specificchanges were not found between control and treated groups in hematology and serum biochemistry. In additionno significant changes of gross body and individual organs weight. Conclusions : These results suggest that water soluble extract of Alismatis Rhizoma has not repeated dose oraltoxicity and oral LD50 value was over 2,000 ㎎/㎏ in SD rats. As a result, we can determine Alismatis Rhizoma isa relatively safe substance.
Abstract
Objectives : This study was carried out to evaluate the repeated dose oral toxicity of Alismatis Rhizoma inSprague-Dawley(SD) rats. Methods : Male and female rats were administered orally with Alismatis Rhizoma water extract of 500 ㎎/㎏(lowdosage group), 1,000 ㎎/㎏(middle dosage group) and 2,000 ㎎/㎏(high dosage group). We daily observed numberof deaths, clinical signs and gross findings for 14 days(twice a day). After 14 days, we measured body and organsweight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specificchanges were not found between control and treated groups in hematology and serum biochemistry. In additionno significant changes of gross body and individual organs weight. Conclusions : These results suggest that water soluble extract of Alismatis Rhizoma has not repeated dose oraltoxicity and oral LD50 value was over 2,000 ㎎/㎏ in SD rats. As a result, we can determine Alismatis Rhizoma isa relatively safe substance.
참고문헌 (17)
- Chang IM, "Current trend of natural products industry and utilization of herbal materials", Korean journal of crop science, 47(1), pp.28~37 (2002)
- 유태우, "독성 간손상 관련 한국인의 약물복용 실태와 건강비용 조사대한간학회지 제 13 권 제 1 호 2007 ; 34-43; 독성 간손상의 진단 및 보고체계 구축을 위한 다기관 공동연구", Clinical and Molecular Hepatology, 13(1), pp.34~43 (2007), 대한간학회
- 김태희, "유통 한약재의 잔류농약 및 잔류이산화황의 함량 분석", 대한본초학회지, 27(6), pp.43~48 (2012), 대한본초학회
- 박해모, "한약이 실험동물의 태자에 미치는 생식독성학적 영향† ―오적산을 중심으로―", 대한예방한의학회지, 12(2), pp.27~35 (2008), 대한예방한의학회
- 김윤배, "상백피의 4주 반복투여독성 평가", Laboratory Animal Research, 20(3), pp.283~290 (2004), 한국실험동물학회
- Seo BI, "A philological study on poisoning and side effects of Alismatis Rhizoma", The Journal of Jeahan Oriental Medical Academy, 10(1), pp.147~156 (2012)
- 박종철, "택사 메탄올 추출물과 주성분의 항산화작용", 생약학회지, 38(4), pp.372~375 (2007), 한국생약학회
- 정향숙, "고지방식이로 유발된 비만에 대한 택사의 항비만 효과", 대한본초학회지, 28(3), pp.95~106 (2013), 대한본초학회
- 이세나, "Effects of Alismatis Rhizoma Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities", Korean Journal of Acupuncture, 28(1), pp.15~22 (2011), 경락경혈학회
- Lee DW, "Separation and Purification of Effective Components from the Alisma orientale and its Application as a Cosmeceutical Ingredient", Soc Cosmet Scientists Korea, 32(1), pp.23~28 (2006)
- Ha HY, "The another name of herbal medicine 1", pp.461~462 (2007), passanpass
- Hu XM, "Zhonghwabencao Jingxuanben 1", pp.1991~1998 (1998), kejichubanshe
- Kim KS, "Present Status and Perspectives of Medicinal Plant Resources in Korea", Research of national Resources, 2, pp.25~41 (1999)
- 권기태, "식약공용 한약재의 관리 방안에 관한 연구", 대한본초학회지, 27(2), pp.25~29 (2012), 대한본초학회
- Duan XY, "A 60 day feeding study of Rhizoma Alismatis Orientalis in SD rats", Chinese Journal of Food Hygiene, 16(2), pp.108~111 (2004)
- Kwon BS, "Effect of Nursery Period on Growth and Yield in Alisma plantago", Korean J Plant Res, 14(2), pp.124~128 (2001)
- Park JH, "A philological study on poisoning and side effects of Alismatis Rhizoma", The Journal of Jeahan Oriental Medical Academy, 10(1), pp.147~156 (2012)