본문 바로가기
KERD
논문KCI 등재

金銀花와 黃芩이 配伍된 處方劑의 抗炎症 效果 硏究

Study on the Anti-inflammatory Effects of the Remedy Prescripted with Lonicera japonica and Scutellaria baicalensis Radix in U937 cells.

1

조회

0

다운로드

3

인용

0

참고문헌

키워드

주제분류한의학

초록

Objective : This study investigated the inflammatory reaction is characterized by over production of inflammatory mediators due to an up-regulation of inflammatory pathways. Methods : We investigated the anti-inflammatory effects of water extracts from Lonicera japonica and Scutellaria baicalensis in lipopolysaccharide (LPS)-stimulated U937 cells. Each extract suppressed the production of inflammatory mediators (NO, IL-1β, TNF-α, and PGE2) and the expression of inducible NO synthase and cyclooxygenase-2 in LPS- stimulated U937 cells in a dose-dependent manner. Results : These suggest that the suppression effects were synergistically increased by their combination. Their combination extract also inhibited NF-κB-DNA complex of NF-κB binding activity and translocation of NF-κB from cytosol to nucleus. Conclusions : Our study suggest that the combination of water-extractable components of L. japonica and S. baicalensis may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.

Abstract

Objective : This study investigated the inflammatory reaction is characterized by over production of inflammatory mediators due to an up-regulation of inflammatory pathways. Methods : We investigated the anti-inflammatory effects of water extracts from Lonicera japonica and Scutellaria baicalensis in lipopolysaccharide (LPS)-stimulated U937 cells. Each extract suppressed the production of inflammatory mediators (NO, IL-1β, TNF-α, and PGE2) and the expression of inducible NO synthase and cyclooxygenase-2 in LPS- stimulated U937 cells in a dose-dependent manner. Results : These suggest that the suppression effects were synergistically increased by their combination. Their combination extract also inhibited NF-κB-DNA complex of NF-κB binding activity and translocation of NF-κB from cytosol to nucleus. Conclusions : Our study suggest that the combination of water-extractable components of L. japonica and S. baicalensis may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.

참고문헌 (39)

  1. Shin MG, "Clinical herbalogy", Youngrimsa, pp.308~321 (1991)
  2. Kang BS, "Clinical with herbal medicine", pp.287~290 (1994), Youngrimsa
  3. Kim CM, "After translation TCM Dictionary", Jeongdam, pp.582~587 (1997)
  4. State Pharmaceutical Administration, "Chinese herbal medicine", pp.1791~1817 (1998), Science and Technology Publisher
  5. Tae BS, "Korean Dictionary of clinical medicine", pp.51~53 (1994), Euiseongdang
  6. Kim HS, "Effects of flos lonicerae water extract on anti-rheumatiod arthritis", pp.2003
  7. Jeong BS, "(Crude drug) illustrates Xiang drug dictionary", pp.864~865 (1990), Youngrimsa
  8. Kim EJ, "Suppression by a sesquiterpene lactone from Carpesium divaricantum of inducible nitric oxide synthase by inhibiting nuclear factor-kB activation", Biochem Pharmarol, 61, pp.903~910 (2001)
  9. Choi SM, "Screening of the Antibacterial Activity of Natural Products against Propionibacterium acnes", Archives of Pharmacal Research, 42, pp.89~94 (1998)
  10. Shin YS, "Experimental study on the Yubaekpi anti-inflammatory and tissue regeneration", J Korean Med Ophthalmol Otolaryngol Dermatol, 14, pp.129~135 (2001)
  11. Baek GH, "Antitumor Effects of the Ethyl Acetate Soluble Fraction of Lonicerae flos on Human Oral Epitheloid Carcinoma Cells", pp.1997
  12. Tae J, "Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema", Clinica Chimica Acta, 330, pp.165~171 (2003)
  13. Lee JH, "Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver", Int J Mol Cell Med, 7, pp.79~83 (2001)
  14. Ohta S, "Protective effects of Taiwan crude drugs on experimental liver injuries Yakugaku Zasshi.", 113, pp.870~880 (1993)
  15. Jang SI, "Aqueous extract from Lonicera japonica flower Inhibits LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages" (2005)
  16. Hu S, "Influence of medicinal herbs on phagocytosis by bovine neutrophils", Zentralbl Veterinarmed A, 39, pp.593~599 (1992)
  17. Tang W, "Chinese Drugs of plant origin", Springer-Verg, pp.919~929 (1992)
  18. Abe K, "Biliary excretion of metabolites of baicalin and baicalein in rats", Chem Pharm Bull(Tokyo), 38(1), pp.209~211 (1990)
  19. Li BQ, "The flavonoid baicalin exhibits anti-inflammatory activity by binding to chemokines", Immunopharmacology, 49(3), pp.295~306 (2000)
  20. Krakauer T, "The flavonoid baicalin inhibits superantigeninduced inflammatory cytokines and chemokines", FEBS Lett, 500(1-2), pp.52~55 (2001)
  21. Chen YC, "Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide", Biochem Pharmacol, 61(11), pp.1417~1427 (2001)
  22. Ahmad N, "Regulation of cyclooxygenase-2 by nitric oxide in activated hepatic macrophages during acute endotoxemia", Journal of Leukocyte Biology, 71, pp.1005~1011 (2002)
  23. Barnes PJ, "Nuclear factor-κB : a pivotal transcription factor in chronic inflammatory diseases", New England Journal Medecine, 336, pp.1066~1071 (1997)
  24. Coker PK, "Pulmonary fibrosis : cytokines in the balance", European Respiratory Journal, 11, pp.1218~1221 (1998)
  25. De Nardin E, "The role of inflammatory and immunological mediators in periodontitis and cardiovascular disease", Annals of Periodontology, 6, pp.3040~ (2001)
  26. Dinarello CA, "Cytokines as endogenous pyrogens", Journal of Infectious Diseases, 179, pp.294~304 (1999)
  27. Feldmann M, "Role of cytokines in rheumatoid arthritis", Annual Review ofImmunology, 14, pp.397~440 (1996)
  28. Isomaki P, "Pro-and anti-inflammatory cytokines in rheumatoid arthritis", Annals of Medicine, 29, pp.499~507 (1997)
  29. Kotake Y, "Inhibition of NF-kappaB, iNOS mRNA, COX2mRNA, and COX catalytic activity by phenyl-N-tert-butylnitrone(PBN)", Biochimica Etbiophysica Acta, 1448, pp.77~84 (1998)
  30. Kroncke KD, "Nitric oxide: cytotoxicity versus cytoprotectionhow, why, when, and where?", Nitric Oxide, 1, pp.107~120 (1997)
  31. Birkenkamp KU, "The p38MAP kinase inhibitor SB203580 enhances nuclear factor-kappa B transcriptional activity by a non-specific effect upon the ERK pathway", Br J Pharmacol, 131, pp.99~107 (2001)
  32. Bian ZM, "Human RPE-monocyte co-culture induces chemokine gene expression through activation of MAPK and NIK cascade", Exp Eye Res, 76, pp.573~583 (2003)
  33. Lee HW, "Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras ERK and NF-kappaB", J Biol Chem, 277, pp.32624~32631 (2002)
  34. Majumdar S, "Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation", J Immunol, 167, pp.2911~2920 (2001)
  35. Garrington TP, "Organization and regulation of mitogen-activated protein kinase signaling pathways", Curr Opin Cell Biol, 11, pp.211~218 (1999)
  36. Mancuso G, "Mitogen-activated protein kinases and NF-kB are involved in TNF-α responses to group B streptococci", J Immunol, 169, pp.1401~1409 (2002)
  37. Sha WC, "Targeted disruption of the p50 subunit of NF-kB leads to multifocal defects in immune responses", Cell, 80, pp.321~330 (1995)
  38. Verma Q, "Nat Rev", Immunol, 2, pp.725~734 (2002)
  39. Jang SI, "Hepatoprotective effect of baicalin, a major flavone from Scutellaria radix, on acetaminophen-induced liver injury in mice", Immunopharmacol Immunotoxicol, 25(4), pp.585~594 (2003)

관련 논문AI추천

이 간행물의 다른 논문 (12건)