金銀花와 黃芩이 配伍된 處方劑의 抗炎症 效果 硏究
Study on the Anti-inflammatory Effects of the Remedy Prescripted with Lonicera japonica and Scutellaria baicalensis Radix in U937 cells.
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Objective : This study investigated the inflammatory reaction is characterized by over production of inflammatory mediators due to an up-regulation of inflammatory pathways. Methods : We investigated the anti-inflammatory effects of water extracts from Lonicera japonica and Scutellaria baicalensis in lipopolysaccharide (LPS)-stimulated U937 cells. Each extract suppressed the production of inflammatory mediators (NO, IL-1β, TNF-α, and PGE2) and the expression of inducible NO synthase and cyclooxygenase-2 in LPS- stimulated U937 cells in a dose-dependent manner. Results : These suggest that the suppression effects were synergistically increased by their combination. Their combination extract also inhibited NF-κB-DNA complex of NF-κB binding activity and translocation of NF-κB from cytosol to nucleus. Conclusions : Our study suggest that the combination of water-extractable components of L. japonica and S. baicalensis may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.
Abstract
Objective : This study investigated the inflammatory reaction is characterized by over production of inflammatory mediators due to an up-regulation of inflammatory pathways. Methods : We investigated the anti-inflammatory effects of water extracts from Lonicera japonica and Scutellaria baicalensis in lipopolysaccharide (LPS)-stimulated U937 cells. Each extract suppressed the production of inflammatory mediators (NO, IL-1β, TNF-α, and PGE2) and the expression of inducible NO synthase and cyclooxygenase-2 in LPS- stimulated U937 cells in a dose-dependent manner. Results : These suggest that the suppression effects were synergistically increased by their combination. Their combination extract also inhibited NF-κB-DNA complex of NF-κB binding activity and translocation of NF-κB from cytosol to nucleus. Conclusions : Our study suggest that the combination of water-extractable components of L. japonica and S. baicalensis may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.
참고문헌 (39)
- Shin MG, "Clinical herbalogy", Youngrimsa, pp.308~321 (1991)
- Kang BS, "Clinical with herbal medicine", pp.287~290 (1994), Youngrimsa
- Kim CM, "After translation TCM Dictionary", Jeongdam, pp.582~587 (1997)
- State Pharmaceutical Administration, "Chinese herbal medicine", pp.1791~1817 (1998), Science and Technology Publisher
- Tae BS, "Korean Dictionary of clinical medicine", pp.51~53 (1994), Euiseongdang
- Kim HS, "Effects of flos lonicerae water extract on anti-rheumatiod arthritis", pp.2003
- Jeong BS, "(Crude drug) illustrates Xiang drug dictionary", pp.864~865 (1990), Youngrimsa
- Kim EJ, "Suppression by a sesquiterpene lactone from Carpesium divaricantum of inducible nitric oxide synthase by inhibiting nuclear factor-kB activation", Biochem Pharmarol, 61, pp.903~910 (2001)
- Choi SM, "Screening of the Antibacterial Activity of Natural Products against Propionibacterium acnes", Archives of Pharmacal Research, 42, pp.89~94 (1998)
- Shin YS, "Experimental study on the Yubaekpi anti-inflammatory and tissue regeneration", J Korean Med Ophthalmol Otolaryngol Dermatol, 14, pp.129~135 (2001)
- Baek GH, "Antitumor Effects of the Ethyl Acetate Soluble Fraction of Lonicerae flos on Human Oral Epitheloid Carcinoma Cells", pp.1997
- Tae J, "Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema", Clinica Chimica Acta, 330, pp.165~171 (2003)
- Lee JH, "Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver", Int J Mol Cell Med, 7, pp.79~83 (2001)
- Ohta S, "Protective effects of Taiwan crude drugs on experimental liver injuries Yakugaku Zasshi.", 113, pp.870~880 (1993)
- Jang SI, "Aqueous extract from Lonicera japonica flower Inhibits LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages" (2005)
- Hu S, "Influence of medicinal herbs on phagocytosis by bovine neutrophils", Zentralbl Veterinarmed A, 39, pp.593~599 (1992)
- Tang W, "Chinese Drugs of plant origin", Springer-Verg, pp.919~929 (1992)
- Abe K, "Biliary excretion of metabolites of baicalin and baicalein in rats", Chem Pharm Bull(Tokyo), 38(1), pp.209~211 (1990)
- Li BQ, "The flavonoid baicalin exhibits anti-inflammatory activity by binding to chemokines", Immunopharmacology, 49(3), pp.295~306 (2000)
- Krakauer T, "The flavonoid baicalin inhibits superantigeninduced inflammatory cytokines and chemokines", FEBS Lett, 500(1-2), pp.52~55 (2001)
- Chen YC, "Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide", Biochem Pharmacol, 61(11), pp.1417~1427 (2001)
- Ahmad N, "Regulation of cyclooxygenase-2 by nitric oxide in activated hepatic macrophages during acute endotoxemia", Journal of Leukocyte Biology, 71, pp.1005~1011 (2002)
- Barnes PJ, "Nuclear factor-κB : a pivotal transcription factor in chronic inflammatory diseases", New England Journal Medecine, 336, pp.1066~1071 (1997)
- Coker PK, "Pulmonary fibrosis : cytokines in the balance", European Respiratory Journal, 11, pp.1218~1221 (1998)
- De Nardin E, "The role of inflammatory and immunological mediators in periodontitis and cardiovascular disease", Annals of Periodontology, 6, pp.3040~ (2001)
- Dinarello CA, "Cytokines as endogenous pyrogens", Journal of Infectious Diseases, 179, pp.294~304 (1999)
- Feldmann M, "Role of cytokines in rheumatoid arthritis", Annual Review ofImmunology, 14, pp.397~440 (1996)
- Isomaki P, "Pro-and anti-inflammatory cytokines in rheumatoid arthritis", Annals of Medicine, 29, pp.499~507 (1997)
- Kotake Y, "Inhibition of NF-kappaB, iNOS mRNA, COX2mRNA, and COX catalytic activity by phenyl-N-tert-butylnitrone(PBN)", Biochimica Etbiophysica Acta, 1448, pp.77~84 (1998)
- Kroncke KD, "Nitric oxide: cytotoxicity versus cytoprotectionhow, why, when, and where?", Nitric Oxide, 1, pp.107~120 (1997)
- Birkenkamp KU, "The p38MAP kinase inhibitor SB203580 enhances nuclear factor-kappa B transcriptional activity by a non-specific effect upon the ERK pathway", Br J Pharmacol, 131, pp.99~107 (2001)
- Bian ZM, "Human RPE-monocyte co-culture induces chemokine gene expression through activation of MAPK and NIK cascade", Exp Eye Res, 76, pp.573~583 (2003)
- Lee HW, "Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras ERK and NF-kappaB", J Biol Chem, 277, pp.32624~32631 (2002)
- Majumdar S, "Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation", J Immunol, 167, pp.2911~2920 (2001)
- Garrington TP, "Organization and regulation of mitogen-activated protein kinase signaling pathways", Curr Opin Cell Biol, 11, pp.211~218 (1999)
- Mancuso G, "Mitogen-activated protein kinases and NF-kB are involved in TNF-α responses to group B streptococci", J Immunol, 169, pp.1401~1409 (2002)
- Sha WC, "Targeted disruption of the p50 subunit of NF-kB leads to multifocal defects in immune responses", Cell, 80, pp.321~330 (1995)
- Verma Q, "Nat Rev", Immunol, 2, pp.725~734 (2002)
- Jang SI, "Hepatoprotective effect of baicalin, a major flavone from Scutellaria radix, on acetaminophen-induced liver injury in mice", Immunopharmacol Immunotoxicol, 25(4), pp.585~594 (2003)
