감맥대조탕이 DNCB로 유발된 생쥐의 아토피피부염에 미치는 영향
Effects of Gammakdaejo-tang(GMD) on DNCB induced Atopic Dermatitis in Mice
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Objectives : Atopic dermatitis is a chronic inflammatory skin disease with frequent relapses. This study was to investigate the effects of Gammakdaejo-tang(GMD) in DNCB induced atopic dermatitis mice. Methods : The study was divided into five comparion groups. 2,4-dinitrochlorobenzene(DNCB) solution was applied to Nc/Nga mice to induce atopic dermatitis, followed by normal group, negative control group with distilled water, positive control group with Dexamethasione and GMD 200㎎/㎏ or 400㎎/㎏. The control group was orally administered 200㎕ once daily for 4 weeks. Visual skin condition, Immunoglobulin E, Histamine, Cytokine, Immune cells, Tissue biomarkers were observed. Results : As a result of the dermatitis score evaluation, it was confirmed that the GMD-administered group improved symptoms compared to the negative control group. As a result of measuring IgE, the GMD-administered group significantly decreased compared to the negative control group. As a result of measuring Histamine, GMD group except 200㎎/㎏ of GMD significantly decreased compared to negative control group. As a result of measuring cytokine, GMD 200㎎/㎏ significantly reduced IL-1β, IL-6 and TNF-α compared to the negative control. 400㎎/㎏ significantly reduced IL-1β, IL-4, IL-5, IL-6, IL-10, TNF-α and significantly increased IL-2, IFN-γ. As a result of confirming the immune cells, all experimental groups showed no difference in basophil, GMD group significantly reduced monocyte and eosinophil compared to negative control group, and GMD 400㎎/㎏ group significantly reduced white blood cell and neutrophil. And significantly increased lymphocytes. As a result of measuring the gene expression level, all GMD group significantly increased TGF-β1 compared with the negative control group, and filaggrin, VEGF and EGF were significantly increased in GMD 400㎎/㎏ group. Epidermis, dermis thickness, and eosinophil infiltration were found to be decreased in all GMD groups compared with the negative control group. Conclusions : GMD is effective in atopic dermatitis by reducing imbalance of immune response of T cells (Th1 / Th2) and reducing skin tissue damage and inflammatory response.
Abstract
Objectives : Atopic dermatitis is a chronic inflammatory skin disease with frequent relapses. This study was to investigate the effects of Gammakdaejo-tang(GMD) in DNCB induced atopic dermatitis mice. Methods : The study was divided into five comparion groups. 2,4-dinitrochlorobenzene(DNCB) solution was applied to Nc/Nga mice to induce atopic dermatitis, followed by normal group, negative control group with distilled water, positive control group with Dexamethasione and GMD 200㎎/㎏ or 400㎎/㎏. The control group was orally administered 200㎕ once daily for 4 weeks. Visual skin condition, Immunoglobulin E, Histamine, Cytokine, Immune cells, Tissue biomarkers were observed. Results : As a result of the dermatitis score evaluation, it was confirmed that the GMD-administered group improved symptoms compared to the negative control group. As a result of measuring IgE, the GMD-administered group significantly decreased compared to the negative control group. As a result of measuring Histamine, GMD group except 200㎎/㎏ of GMD significantly decreased compared to negative control group. As a result of measuring cytokine, GMD 200㎎/㎏ significantly reduced IL-1β, IL-6 and TNF-α compared to the negative control. 400㎎/㎏ significantly reduced IL-1β, IL-4, IL-5, IL-6, IL-10, TNF-α and significantly increased IL-2, IFN-γ. As a result of confirming the immune cells, all experimental groups showed no difference in basophil, GMD group significantly reduced monocyte and eosinophil compared to negative control group, and GMD 400㎎/㎏ group significantly reduced white blood cell and neutrophil. And significantly increased lymphocytes. As a result of measuring the gene expression level, all GMD group significantly increased TGF-β1 compared with the negative control group, and filaggrin, VEGF and EGF were significantly increased in GMD 400㎎/㎏ group. Epidermis, dermis thickness, and eosinophil infiltration were found to be decreased in all GMD groups compared with the negative control group. Conclusions : GMD is effective in atopic dermatitis by reducing imbalance of immune response of T cells (Th1 / Th2) and reducing skin tissue damage and inflammatory response.
참고문헌 (28)
- Kim KB, "Hanbangsoacheongsonyeonuihak", pp.231~232 (2015), EUi Sung Dang Publishing Co
- Kauppi S, "Adult Patients with Atopic Eczema have a High Burden of Psychiatric Disease : A Finnish Nationwide Registry Study", Acta Derm Venereol, 99(7), pp.647~651 (2019)
- Kim KB, "Hanbangsoacheongsonyeonuihak", pp.234~235 (2015), EUi Sung Dang Publishing Co
- 정아람, "황련 추출물의 아토피피부염 유발 생쥐에서 피부손상 완화 효과", 동의생리병리학회지, 33(2), pp.102~108 (2019), 한의병리학회[DOI]
- Lee E, "Gammakdaejo-tang(甘麥大棗湯)'s effect on alcoholic liver disease - 1 case", Journal Of The Institute Oriental Medicine, 3, pp.45~53 (2001)
- Textbook Compilation Committee of National University of Korean medicine, "Herbalogy", pp.584~587 (2012), Yeong-Lim publishing
- 안상현, "황련감초 하태독법의 피부 지방장벽형성 증진효과", 대한한방소아과학회지, 31(3), pp.14~23 (2017), 대한한방소아과학회
- 최소영, "Hairless 마우스에서 대추 열수추출물의 피부내 염증 및 장벽 손상 완화 효과", 환경독성보건학회지, 24(4), pp.351~357 (2009), 환경독성보건학회
- 백현, "甘麥大棗湯이 신체부동 스트레스 白鼠에 미치는 影響", 대한한의학회지, 28(3), pp.183~196 (2007), 대한한의학회
- 백혜숙, "소맥추출물과 그 구성성분을 이용한 허혈성 급성신부전 치료", 석사, 의학, pp.2011
- 원종현, "알레르기질환 및 아토피피부염에서 가려움증의 병인 및 치료적 접근", 천식 및 알레르기, 30(3), pp.184~192 (2010), 대한천식알레르기학회
- 이유찬, "감맥대조탕 복합추출물의 만성스트레스 유발 생쥐의 항우울 작용에 관한 실험연구", 동의신경정신과학회지, 29(1), pp.57~68 (2018), 대한한방신경정신과학회[DOI]
- Jeong HW, "Experimental Effects of Gammaekdaejo-tang and It's Constituent Herbs on the Regional Cerebral Blood Flow and Cardiacvascular System", Korean Journal of Oriental Physiology &Pathology, 15(4), pp.590~594 (2001)
- 한태영, "아토피피부염의 치료", 대한피부과학회지, 56(10), pp.581~593 (2018), 대한피부과학회
- 김건우, "아토피피부염 동물 병태 모델에서 아토탕의 개선효과", 대한본초학회지, 31(4), pp.79~85 (2016), 대한본초학회
- Yeo EJ, "Topical Application of Atopy cream-Jawoongo ointment(A-J)of Ointment Inhibits Biostir mite antigen cream caused Atopy Dermatitis by Local Action in NC", Nga Mice, 2008, pp.17(2), 185~198
- 심부용, "아토피 피부염을 유발한 마우스에서 백선피지부자복합방의 항염증 효과", 대한본초학회지, 29(3), pp.51~58 (2014), 대한본초학회
- 민들레, "甘松香 (감송향)이 아토피樣 (양) 피부염에 미치는 영향", 대한한방소아과학회지, 26(2), pp.13~24 (2012), 대한한방소아과학회[DOI]
- Textbook compilation committee of National university korean medicine Dermatology & Surgery, "Text of Traditional Korean Dermatology & Surgery", pp.355~361 (2008), Busan:Sunwoo publishing
- 김계순, "화장품 소재로서 감초 추출물의 생리활성", 아시안뷰티화장품학술지, 15(1), pp.11~22 (2017), 한국피부과학연구원[DOI]
- 김지은, "大靑龍湯이 DNCB로 유발된 생쥐의 아토피피부염에 미치는 영향", 한방안이비인후피부과학회지, 32(3), pp.58~76 (2019), 대한한방안이비인후피부과학회[DOI]
- 이병웅, "아토피피부염 동물 병태 모델에서 石紫解肌湯의 면역조절작용에 관한 연구", 동의생리병리학회지, 26(4), pp.446~454 (2012), 한의병리학회[DOI]
- Leung DYM, "Cellular and immunologic mechanisms in atopic dermatitis", Journal of the American Academy of Dermatology, 44(1), pp.1~12 (2001)
- Pastore S, "Pathogenic mechanisms of atopic dermatitis", Arch Immunol Ther Exp (Warsz), 48(6), pp.497~504 (2000)
- Romagnani S, "immunologic influences on allergy and the Th1", Th2 balance, 2004, pp.113(3), 395~400
- Kang RY, "The effects of HYT on various immunological factors related to pathogenesis of allergic dermatitis in NC", Nga mice caused by Biostir AD, 2009, pp.18(2), 47~62
- 김범회, "흰쥐의 전층피부상처 동물모델에서 消風散이 VEGF 및 TGF-β1 발현에 미치는 영향", 대한한의학 방제학회지, 25(2), pp.123~134 (2017), 대한한의학방제학회[DOI]
- 이한일, "한국인 아토피피부염 환자에서의 필라그린 유전자 변이 여부에 따른 임상 양상 비교", 대한피부과학회지, 57(7), pp.363~370 (2019), 대한피부과학회