MicroScan과 Phoenix 자동화 기기의 Extended-Spectrum β-Lactamase-생성 Escherichia coli와 Klebsiella pneumoniae 검출능 비교
Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae
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초록
이 연구의 목적은 extended-spectrum β-lactamase (ESBL)을 생성하는 Escherichia coli와 Klebsiella pneumoniae를 검출하는데 있어 MicroScan (Siemens Healthcare, USA)과 Phoenix (Becton Dickinson Diagnostic Systems, USA) 검사기기의 수행능을 평가하는 데 있다. E. coli 18주와 K. pneumoniae 26주를 대상으로 MicroScan과Phoenix, double-disk synergy test (DDST)를 이용하여ESBL 생성주를 검출하였고 이들이 생성하는 ESBL형을 중합효소연쇄반응 직접 염기순서분석법으로 검사하였다. ESBL 유전자는검사한 44주 중 38 (86.4%)주에서 검출되었다. MicroScan과 Phoenix,DDST의 ESBL 검출 민감도는 각각 94.6%와 79%, 89.5%이었다. MicroScan과 Phoenix 검사기기는 둘다 임상분리주의 ESBL 검출에 적합하다고 여겨졌다.
Abstract
The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems,USA) automated systems for the detection of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.
참고문헌 (8)
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