Correlation between Clinical Severity and Cycle Threshold Values of Real-time RT-PCR at the Time of Admission in COVID-19 Patients
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Background: The viral load of the patient can be deduced from the cycle threshold (Ct) value. In this study, the correlation between the initial Ct value of the SARS-CoV-2 real-time reverse transcription polymerase chain reaction (RT-PCR) and the clinical features in hospitalized patients in Daegu, South Korea, was explored. Methods: We investigated the clinical and RT-PCR data of 750 patients with COVID-19 (Coronavirus disease-19) at admission in eight hospitals in Daegu, Korea, and assessed the correlation between the Ct values and COVID-19 severity. Results: The Ct values for the four genes showed a statistically significant difference (P<0.001) within the same specimen type. Among the 716 patients classified based on clinical severity, the Ct value was a significant predictor of disease severity at the time of admission. The Ct values of the E, RdRP, and N genes showed significant differences among the eight severity groups (P<0.001). Compared to patients in the non-severe group (categories 1 to 4), those in the severe group (categories 5 to 8) exhibited a relatively lower distribution of Ct values for the E (P<0.001), RdRP (P=0.029), ORF1a (P=0.009), and N genes (P=0.003). Conclusions: The Ct value, which reflects the amount of virus, can serve as a supportive parameter for predicting the severity of COVID-19 in patients at the time of admission.
Abstract
Background: The viral load of the patient can be deduced from the cycle threshold (Ct) value. In this study, the correlation between the initial Ct value of the SARS-CoV-2 real-time reverse transcription polymerase chain reaction (RT-PCR) and the clinical features in hospitalized patients in Daegu, South Korea, was explored. Methods: We investigated the clinical and RT-PCR data of 750 patients with COVID-19 (Coronavirus disease-19) at admission in eight hospitals in Daegu, Korea, and assessed the correlation between the Ct values and COVID-19 severity. Results: The Ct values for the four genes showed a statistically significant difference (P<0.001) within the same specimen type. Among the 716 patients classified based on clinical severity, the Ct value was a significant predictor of disease severity at the time of admission. The Ct values of the E, RdRP, and N genes showed significant differences among the eight severity groups (P<0.001). Compared to patients in the non-severe group (categories 1 to 4), those in the severe group (categories 5 to 8) exhibited a relatively lower distribution of Ct values for the E (P<0.001), RdRP (P=0.029), ORF1a (P=0.009), and N genes (P=0.003). Conclusions: The Ct value, which reflects the amount of virus, can serve as a supportive parameter for predicting the severity of COVID-19 in patients at the time of admission.
참고문헌 (24)
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