Superoxide Dismutase 1 Inhibits Alpha-Melanocyte Stimulating Hormone and Ultraviolet B-Induced Melanogenesis in Murine Skin
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Background: Over the last decade, the incidence of ultravioletB (UVB)-related skin problems has increased. Oxidativestress caused by UVB induces the secretion of melanocytegrowth and activating factors from keratinocytes, whichresults in the formation of cutaneous hyperpigmentation. Therefore, increasing the antioxidant abilities of skin cells isthought to be a beneficial strategy for the development ofsunscreen agents. Superoxide dismutase 1 (SOD1) is anantioxidant enzyme that is known to exhibit antioxidantproperties. Objective: The purpose of this study was to investigatethe effect of SOD1 on alpha-melanocyte stimulatinghormone (α-MSH) and UVB-induced melanogenesis inB16F10 melanoma cells and HRM-2 melanin-possessinghairless mice. Methods: The inhibitory effect of SOD1 ontyrosinase activity was evaluated in a cell-free system. Additional experiments were performed using B16F10melanoma cells to demonstrate the effects of SOD1 in vitro,and HRM-2 melanin-possessing hairless mice were used toevaluate the antimelanogenic effects of SOD1 in vivo. Results: We found that SOD1 inhibited melanin productionin a dose-dependent manner without causing cytotoxicity inB16F10 melanoma cells. SOD1 did not inhibit tyrosinaseactivity under cell-free conditions. The results indicate thatSOD1 may reduce pigmentation by an indirect, nonenzymaticmechanism. We also found that SOD1 decreasedUVB-induced melanogenesis in HRM-2 melanin-possessinghairless mice, as visualized through hematoxylin and eosinstaining and Fontana-Masson staining. Conclusion: Ourresults indicate that SOD1 has an inhibitory effect on α-MSHand UVB-induced melanogenesis, indicating that SOD1 maybe a promising sunscreen agent.
Abstract
Background: Over the last decade, the incidence of ultravioletB (UVB)-related skin problems has increased. Oxidativestress caused by UVB induces the secretion of melanocytegrowth and activating factors from keratinocytes, whichresults in the formation of cutaneous hyperpigmentation. Therefore, increasing the antioxidant abilities of skin cells isthought to be a beneficial strategy for the development ofsunscreen agents. Superoxide dismutase 1 (SOD1) is anantioxidant enzyme that is known to exhibit antioxidantproperties. Objective: The purpose of this study was to investigatethe effect of SOD1 on alpha-melanocyte stimulatinghormone (α-MSH) and UVB-induced melanogenesis inB16F10 melanoma cells and HRM-2 melanin-possessinghairless mice. Methods: The inhibitory effect of SOD1 ontyrosinase activity was evaluated in a cell-free system. Additional experiments were performed using B16F10melanoma cells to demonstrate the effects of SOD1 in vitro,and HRM-2 melanin-possessing hairless mice were used toevaluate the antimelanogenic effects of SOD1 in vivo. Results: We found that SOD1 inhibited melanin productionin a dose-dependent manner without causing cytotoxicity inB16F10 melanoma cells. SOD1 did not inhibit tyrosinaseactivity under cell-free conditions. The results indicate thatSOD1 may reduce pigmentation by an indirect, nonenzymaticmechanism. We also found that SOD1 decreasedUVB-induced melanogenesis in HRM-2 melanin-possessinghairless mice, as visualized through hematoxylin and eosinstaining and Fontana-Masson staining. Conclusion: Ourresults indicate that SOD1 has an inhibitory effect on α-MSHand UVB-induced melanogenesis, indicating that SOD1 maybe a promising sunscreen agent.
참고문헌 (33)
- Nole G, "An analysis of cumulative lifetime solar ultraviolet radiation exposure and the benefits of daily sun protection", Dermatol Ther, 17(Suppl 1), pp.57~62 (2004)
- Gonzalez Maglio DH, "Skin damage and mitochondrial dysfunction after acute ultraviolet B irradiation: relationship with nitric oxide production", Photodermatol Photoimmunol Photomed, 21, pp.311~317 (2005)
- Matsumura Y, "Toxic effects of ultraviolet radiation on the skin", Toxicol Appl Pharmacol, 195, pp.298~308 (2004)
- Gloster HM Jr, "The epidemiology of skin cancer", Dermatol Surg, 22, pp.217~226 (1996)
- Fisher MS, "Suppressor T lymphocytes control the development of primary skin cancers in ultraviolet-irradiated mice", Science, 216, pp.1133~1134 (1982)
- Ullrich SE, "Mechanisms underlying UV-induced immune suppression", Mutat Res, 571, pp.185~205 (2005)
- Mørch CD, "The UVB cutaneous inflammatory pain model: a reproducibility study in healthy volunteers", Int J Physiol Pathophysiol Pharmacol, 5, pp.203~215 (2013)
- Friedmann PS, "Ultraviolet radiation directly induces pigment production by cultured human melanocytes", J Cell Physiol, 133, pp.88~94 (1987)
- Gilchrest BA, "A review of skin ageing and its medical therapy", Br J Dermatol, 135, pp.867~875 (1996)
- Kadekaro AL, "Cutaneous photobiology. The melanocyte vs. the sun: who will win the final round?", Pigment Cell Res, 16, pp.434~447 (2003)
- Meredith P, "The physical and chemical properties of eumelanin", Pigment Cell Res, 19, pp.572~594 (2006)
- Hill HZ, "UVA, pheomelanin and the carcinogenesis of melanoma", Pigment Cell Res, 13(Suppl 8), pp.140~144 (2000)
- Rouzaud F, "MC1R and the response of melanocytes to ultraviolet radiation", Mutat Res, 571, pp.133~152 (2005)
- Yamaguchi Y, "Human skin responses to UV radiation: pigment in the upper epidermis protects against DNA damage in the lower epidermis and facilitates apoptosis", FASEB J, 20, pp.1486~1488 (2006)
- Miyamura Y, "Regulation of human skin pigmentation and responses to ultraviolet radiation", Pigment Cell Res, 20, pp.2~13 (2007)
- Svobodová A, "Attenuation of UVA-induced damage to human keratinocytes by silymarin", J Dermatol Sci, 46, pp.21~30 (2007)
- Tobi SE, "The green tea polyphenol, epigallocatechin-3-gallate, protects against the oxidative cellular and genotoxic damage of UVA radiation", Int J Cancer, 102, pp.439~444 (2002)
- Eller MS, "DNA damage enhances melanogenesis", Proc Natl Acad Sci U S A, 93, pp.1087~1092 (1996)
- Halaban R, "Tyrosinase activity and abundance in Cloudman melanoma cells", Arch Biochem Biophys, 230, pp.383~387 (1984)
- Hunt G, "Nle4DPhe7alpha-melanocyte-stimulating hormone increases the eumelanin: phaeomelanin ratio in cultured human melanocytes", J Invest Dermatol, 104, pp.83~85 (1995)
- Wong G, "Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in Cloudman S91 melanoma cells", Nature, 255, pp.644~646 (1975)
- Matsuda H, "Studies of cuticle drugs from natural sources. IV. Inhibitory effects of some Arctostaphylos plants on melanin biosynthesis", Biol Pharm Bull, 19, pp.153~156 (1996)
- Tobin D, "The superoxide anion may mediate short- but not long-term effects of ultraviolet radiation on melanogenesis", Exp Dermatol, 3, pp.99~105 (1994)
- Chung KW, "Evaluation of in vitro and in vivo anti-melanogenic activity of a newly synthesized strong tyrosinase inhibitor (E)-3-(2,4dihydroxybenzylidene)pyrrolidine-2,5-dione (3-DBP)", Biochim Biophys Acta, 1820, pp.962~969 (2012)
- Song K, "Comparison of the antimelanogenic effects of p-coumaric acid and its methyl ester and their skin permeabilities", J Dermatol Sci, 63, pp.17~22 (2011)
- Pence BC, "Effects of single-dose ultraviolet radiation on skin superoxide dismutase, catalase, and xanthine oxidase in hairless mice", J Invest Dermatol, 95, pp.213~216 (1990)
- Okada K, "Time-dependent effect of chronic UV irradiation on superoxide dismutase and catalase activity in hairless mice skin", J Dermatol Sci, 8, pp.183~186 (1994)
- Kvam E, "Pigmented melanocytes are protected against ultraviolet-A-induced membrane damage", J Invest Dermatol, 121, pp.564~569 (2003)
- Zbytek B, "Current concepts of metastasis in melanoma", Expert Rev Dermatol, 3, pp.569~585 (2008)
- Peng LH, "Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin", Phytomedicine, 20, pp.1082~1087 (2013)
- Kumar KJ, "in vitro and in vivo studies disclosed the depigmenting effects of gallic acid: a novel skin lightening agent for hyperpigmentary skin diseases", Biofactors, 39, pp.259~270 (2013)
- Marshall CJ, "Specificity of receptor tyrosine kinase signaling:transient versus sustained extracellular signal-regulated kinase activation", Cell, 80, pp.179~185 (1995)
- Lei TC, "A melanocytekeratinocyte coculture model to assess regulators of pigmentation in vitro", Anal Biochem, 305, pp.260~268 (2002)