Tranexamic Acid Diminishes Laser-Induced Melanogenesis
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초록
Background: The treatment of post-inflammatory hyperpigmentation (PIH) remains challenging. Tranexamic acid, a well-known anti-fibrinolytic drug, has recently demonstrated a curative effect towards melasma and ultraviolet- induced PIH in Asian countries. However, the precise mechanism of its inhibitory effect on melanogenesis is not fully understood. Objective: In order to clarify the inhibitory effect of tranexamic acid on PIH, we investigated its effects on mouse melanocytes (i.e., melan-a cells) and human melanocytes. Methods: Melan-a cells and human melanocytes were cultured with fractional CO2 laser-treated keratinocyte- conditioned media. Melanin content and tyrosinase activity were evaluated in cells treated with or without tranexamic acid. Protein levels of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 were evaluated in melan-a cells. Signaling pathway molecules involved in melanogenesis in melanoma cells were also investigated. Results: Tranexamic acid-treated melanocytes exhibited reduced melanin content and tyrosinase activity. Tranexamic acid also decreased tyrosinase, TRP-1, and TRP-2 protein levels. This inhibitory effect on melanogenesis was considered to be involved in extracellular signal-regulated kinase signaling pathways and subsequently microphthalmia-associated transcription factor degradation. Conclusion: Tranexamic acid may be an attractive candidate for the treatment of PIH.
Abstract
Background: The treatment of post-inflammatory hyperpigmentation (PIH) remains challenging. Tranexamic acid, a well-known anti-fibrinolytic drug, has recently demonstrated a curative effect towards melasma and ultraviolet- induced PIH in Asian countries. However, the precise mechanism of its inhibitory effect on melanogenesis is not fully understood. Objective: In order to clarify the inhibitory effect of tranexamic acid on PIH, we investigated its effects on mouse melanocytes (i.e., melan-a cells) and human melanocytes. Methods: Melan-a cells and human melanocytes were cultured with fractional CO2 laser-treated keratinocyte- conditioned media. Melanin content and tyrosinase activity were evaluated in cells treated with or without tranexamic acid. Protein levels of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 were evaluated in melan-a cells. Signaling pathway molecules involved in melanogenesis in melanoma cells were also investigated. Results: Tranexamic acid-treated melanocytes exhibited reduced melanin content and tyrosinase activity. Tranexamic acid also decreased tyrosinase, TRP-1, and TRP-2 protein levels. This inhibitory effect on melanogenesis was considered to be involved in extracellular signal-regulated kinase signaling pathways and subsequently microphthalmia-associated transcription factor degradation. Conclusion: Tranexamic acid may be an attractive candidate for the treatment of PIH.
참고문헌 (18)
- Gupta AK, "The treatment of melasma: a review of clinical trials", J Am Acad Dermatol, 55, pp.1048~1065 (2006)
- Costin GE, "Human skin pigmentation:melanocytes modulate skin color in response to stress", FASEB J, 21, pp.976~994 (2007)
- Ando H, "Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase", J Invest Dermatol, 127, pp.751~761 (2007)
- Lee JH, "Localized intradermal microinjection of tranexamic acid for treatment of melasma in Asian patients: a preliminary clinical trial", Dermatol Surg, 32, pp.626~631 (2006)
- Li D, "Tranexamic acid can treat ultraviolet radiation-induced pigmentation in guinea pigs", Eur J Dermatol, 20, pp.289~292 (2010)
- Maeda K, "Topical trans-4-aminomethylcyclohexanecarboxylic acid prevents ultraviolet radiation-induced pigmentation", J Photochem Photobiol B, 47, pp.136~141 (1998)
- Maeda K, "Mechanism of the inhibitory effect of tranexamic acid on melanogenesis in cultured human melanocytes in the presence of keratinocyte-conditioned medium", J Health Sci, 53, pp.389~396 (2007)
- Horrow JC, "Hemostatic effects of tranexamic acid and desmopressin during cardiac surgery", Circulation, 84, pp.2063~2070 (1991)
- Park HY, "Fitzpatrick's dermatology in general medicine", pp.591~607 (2008), McGraw-Hill
- Kim DS, "Sphingosylphosphorylcholine-induced ERK activation inhibits melanin synthesis in human melanocytes", Pigment Cell Res, 19, pp.146~153 (2006)
- Dunn CJ, "Tranexamic acid: a review of its use in surgery and other indications", Drugs, 57, pp.1005~1032 (1999)
- Morelli JG, "Influence of inflammatory mediators and cytokines on human melanocyte function", J Invest Dermatol, 100(2 Suppl), pp.191S~195S (1993)
- Starner RJ, "PGE(2) is a UVR-inducible autocrine factor for human melanocytes that stimulates tyrosinase activation", Exp Dermatol, 19, pp.682~684 (2010)
- Yun WJ, "Epidermal growth factor and epidermal growth factor signaling attenuate laser-induced melanogenesis", Dermatol Surg, 39, pp.1903~1911 (2013)
- Burd A, "A study of Q-switched Nd:YAG laser irradiation and paracrine function in human skin cells", Photodermatol Photoimmunol Photomed, 21, pp.131~137 (2005)
- Chang TS, "An updated review of tyrosinase inhibitors", Int J Mol Sci, 10, pp.2440~2475 (2009)
- Hemesath TJ, "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes", Nature, 391, pp.298~301 (1998)
- Wu M, "c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi", Genes Dev, 14, pp.301~312 (2000)