Magnesium Ascorbyl Phosphate Regulates the Expression of Inflammatory Biomarkers in Cultured Sebocytes
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Background: Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects. Objective: We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes. Methods: Reverse transcription- polymerase chain reaction (RT-PCR) and enzyme- linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10−2 M), lipopolysaccharide (LPS) (5 μg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted. Results: The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS. Conclusion: MAP may be an effective alternative agent to improve inflammatory reactions in acne.
Abstract
Background: Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects. Objective: We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes. Methods: Reverse transcription- polymerase chain reaction (RT-PCR) and enzyme- linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10−2 M), lipopolysaccharide (LPS) (5 μg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted. Results: The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS. Conclusion: MAP may be an effective alternative agent to improve inflammatory reactions in acne.
참고문헌 (18)
- Winston MH, "Acne vulgaris. Pathogenesis and treatment", Pediatr Clin North Am, 38, pp.889~903 (1991)
- Zouboulis CC, "Acne and sebaceous gland function", Clin Dermatol, 22, pp.360~366 (2004)
- Zouboulis CC, "Human skin stem cells and the ageing process", Exp Gerontol, 43, pp.986~997 (2008)
- Park YK, "Whitening effect of cosmetics containing magnesium l-ascorbyl-2-phosphate (VC-PMG, vitamin C derivatives) assessed by colorimeter", Ann Dermatol, 14, pp.63~70 (2002)
- Alestas T, "Enzymes involved in the biosynthesis of leukotriene B4 and prostaglandin E2 are active in sebaceous glands", J Mol Med (Berl), 84, pp.75~87 (2006)
- Corbel M, "Repeated endotoxin exposure induces interstitial fibrosis associated with enhanced gelatinase (MMP-2 and MMP-9) activity", Inflamm Res, 50, pp.129~135 (2001)
- Iwata C, "Augmentation of lipogenesis by 15-deoxy-Delta12,14-prostaglandin J2 in hamster sebaceous glands: identification of cytochrome P-450-mediated 15-deoxy-Delta12,14-prostaglandin J2 production", J Invest Dermatol, 125, pp.865~872 (2005)
- Kim J, "Review of the innate immune response in acne vulgaris: activation of Toll-like receptor 2 in acne triggers inflammatory cytokine responses", Dermatology, 211, pp.193~198 (2005)
- Nagy I, "Propionibacterium acnes and lipopolysaccharide induce the expression of antimicrobial peptides and proinflammatory cytokines", chemokines in human sebocytes, 2006, pp.8, 2195~2205
- Oeff MK, "Differential regulation of Toll-like receptor and CD14 pathways by retinoids and corticosteroids in human sebocytes", Dermatology, 213, pp.266~ (2006)
- Triantafilou M, "The dynamics of LPS recognition:complex orchestration of multiple receptors", J Endotoxin Res, 11, pp.5~11 (2005)
- Bojar RA, "Acne and Propionibacterium acnes", Clin Dermatol, 22, pp.375~379 (2004)
- Böni R, "Treatment of gram-negative folliculitis in patients with acne", Am J Clin Dermatol, 4, pp.273~276 (2003)
- Zhou BR, "Palmitic acid induces production of proinflammatory cytokines interleukin-6, interleukin-1β, and tumor necrosis factor-α via a NF-κB-dependent mechanism in HaCaT keratinocytes", Mediators Inflamm, 2013, pp.530429~ (2013)
- Neely AN, "Gelatinase activity in keloids and hypertrophic scars", Wound Repair Regen, 7, pp.166~171 (1999)
- Papakonstantinou E, "Matrix metalloproteinases of epithelial origin in facial sebum of patients with acne and their regulation by isotretinoin", J Invest Dermatol, 125, pp.673~684 (2005)
- Tanriverdi-Akhisaroglu S, "Matrix metalloproteinase-2 and -9 activities in human keloids, hypertrophic and atrophic scars: a pilot study", Cell Biochem Funct, 27, pp.81~87 (2009)
- Akitomo Y, "Effects of UV irradiation on the sebaceous gland and sebum secretion in hamsters", J Dermatol Sci, 31, pp.151~159 (2003)