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Preferential Recognition of Hydroxyl Radical-Modified Superoxide Dismutase by Circulating Autoantibodies in Patients with Alopecia Areata

Abdullateef A. Alzolibani
대한피부과학회 (Korean Dermalogical Association)|2014.10|Annals of Dermatology|제26권 제5호|pp. 576-583

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참고문헌

키워드

Reactive oxygen speciesSuperoxide dismutaseAutoimmunityAlopecia areataㆍOH-SOD
주제분류피부과학

초록

Background: Alopecia areata (AA) is a common form oflocalized, non-scarring hair loss. The cause of AA isunknown but reports suggest an autoimmune etiology,where oxygen free radicals play an important role. Objective: The aim of this study was to investigate the role ofa hydroxyl radicals (ㆍOH)-modified antioxidant enzyme,superoxide dismutase (SOD), in AA autoimmunity. Methods:SOD was modified by ㆍOH radicals. Binding characteristicsof autoantibodies in AA patients (n=26) against ㆍOHmodifiedSOD (ㆍOH-SOD) were evaluated by immunoassaysand the results were compared with those of healthy,age-matched controls (n=30). The effects of ㆍOH radicalson immunoglobulin G (IgG) isolated from AA patients werestudied. Results: Highly specific binding to ㆍOH-SOD wasobserved in 32% of the samples of patient sera, whereasnormal human sera showed negligible binding with eitherantigen. Competitive inhibition immunoassays reiteratedthe results from direct binding. Protein-A-purified IgG fromAA patients (AA-IgG) also showed strong binding to ㆍOHSODas compared to IgG from normal human controls (p＜0.001). In addition, AA-IgG from patients with alopeciauniversalis recognized ㆍOH-SOD to a greater extent thandid AA-IgG from patients with the patchy, persistent type ofalopecia. Furthermore, sera from AA patients had lowerlevels of SOD activity as compared to control sera. Conclusion: This is the first report showing an associationbetween ㆍOH-modified SOD and AA. These novel resultsdemonstrate that ㆍOH radical-mediated changes in SODpresent unique neo-epitopes that might contribute toantigen-driven antibody induction in AA.

Abstract

Background: Alopecia areata (AA) is a common form oflocalized, non-scarring hair loss. The cause of AA isunknown but reports suggest an autoimmune etiology,where oxygen free radicals play an important role. Objective: The aim of this study was to investigate the role ofa hydroxyl radicals (ㆍOH)-modified antioxidant enzyme,superoxide dismutase (SOD), in AA autoimmunity. Methods:SOD was modified by ㆍOH radicals. Binding characteristicsof autoantibodies in AA patients (n=26) against ㆍOHmodifiedSOD (ㆍOH-SOD) were evaluated by immunoassaysand the results were compared with those of healthy,age-matched controls (n=30). The effects of ㆍOH radicalson immunoglobulin G (IgG) isolated from AA patients werestudied. Results: Highly specific binding to ㆍOH-SOD wasobserved in 32% of the samples of patient sera, whereasnormal human sera showed negligible binding with eitherantigen. Competitive inhibition immunoassays reiteratedthe results from direct binding. Protein-A-purified IgG fromAA patients (AA-IgG) also showed strong binding to ㆍOHSODas compared to IgG from normal human controls (p＜0.001). In addition, AA-IgG from patients with alopeciauniversalis recognized ㆍOH-SOD to a greater extent thandid AA-IgG from patients with the patchy, persistent type ofalopecia. Furthermore, sera from AA patients had lowerlevels of SOD activity as compared to control sera. Conclusion: This is the first report showing an associationbetween ㆍOH-modified SOD and AA. These novel resultsdemonstrate that ㆍOH radical-mediated changes in SODpresent unique neo-epitopes that might contribute toantigen-driven antibody induction in AA.

참고문헌 (33)

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