Citron Essential Oils Alleviate the Mediators Related to Rosacea Pathophysiology in Epidermal Keratinocytes
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Background: Citron is well known for an abundance of antioxidative and anti-inflammatory ingredients such as vitamin C, polyphenol compounds, flavonoids, and limonoids. Objective: In this study, we aimed to evaluate the effects of citron essential oils on rosacea mediators in activated keratinocytes in vitro. Methods: Normal human epidermal keratinocytes (NHEKs) were stimulated with 1α, 25-dihydroxyvitamin D3 (VD3) and interleukin 33 (IL-33) with LL-37 to induce rosacea mediators such as kallikrein 5 (KLK5), cathelicidin, vascular endothelial growth factor (VEGF), and transient receptor potential vanilloid 1 (TRPV1). These mediators were analyzed by performing reverse-transcription polymerase chain reaction (PCR), quantitative real-time PCR, immunocytofluorescence and enzyme-linked immunosorbent assay after NHEKs were treated with citron seed and unripe citron essential oils. Results: The messenger RNA (mRNA) and protein levels of KLK5 and LL-37 induced by VD3 were suppressed by citron seed and unripe citron essential oils. Furthermore, the mRNA and protein levels of VEGF and TRPV1 induced by IL-33 with LL-37 were also suppressed by citron essential oils. Conclusion: These results show that citron essential oils have suppressive effects on rosacea mediators in activated epidermal keratinocytes, which indicates that the citron essential oils may be valuable adjuvant therapeutic agents for rosacea.
Abstract
Background: Citron is well known for an abundance of antioxidative and anti-inflammatory ingredients such as vitamin C, polyphenol compounds, flavonoids, and limonoids. Objective: In this study, we aimed to evaluate the effects of citron essential oils on rosacea mediators in activated keratinocytes in vitro. Methods: Normal human epidermal keratinocytes (NHEKs) were stimulated with 1α, 25-dihydroxyvitamin D3 (VD3) and interleukin 33 (IL-33) with LL-37 to induce rosacea mediators such as kallikrein 5 (KLK5), cathelicidin, vascular endothelial growth factor (VEGF), and transient receptor potential vanilloid 1 (TRPV1). These mediators were analyzed by performing reverse-transcription polymerase chain reaction (PCR), quantitative real-time PCR, immunocytofluorescence and enzyme-linked immunosorbent assay after NHEKs were treated with citron seed and unripe citron essential oils. Results: The messenger RNA (mRNA) and protein levels of KLK5 and LL-37 induced by VD3 were suppressed by citron seed and unripe citron essential oils. Furthermore, the mRNA and protein levels of VEGF and TRPV1 induced by IL-33 with LL-37 were also suppressed by citron essential oils. Conclusion: These results show that citron essential oils have suppressive effects on rosacea mediators in activated epidermal keratinocytes, which indicates that the citron essential oils may be valuable adjuvant therapeutic agents for rosacea.
참고문헌 (41)
- Sawamura M, "Inhibitory effect of yuzu essential oil on the formation of N-nitrosodimethylamine in vegetables", J Agric Food Chem, 53, pp.4281~4287 (2005)
- Yoo KM, "Variation in major antioxidants and total antioxidant activity of Yuzu (Citrus junos Sieb ex Tanaka) during maturation and between cultivars", J Agric Food Chem, 52, pp.5907~5913 (2004)
- Zou Z, "Antioxidant activity of citrus fruits", Food Chem, 196, pp.885~896 (2016)
- Hirota R, "Anti‐inflammatory Effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils", J Food Sci, 75, pp.H87~H92 (2010)
- Crawford GH, "Rosacea: I. Etiology, pathogenesis, and subtype classification", J Am Acad Dermatol, 51, pp.327~341 (2004)
- Schwab VD, "Neurovascular and neuroimmune aspects in the pathophysiology of rosacea", J Investig Dermatol Symp Proc, 15, pp.53~62 (2011)
- Two AM, "Kallikrein 5-mediated inflammation in rosacea: clinically relevant correlations with acute and chronic manifestations in rosacea and how individual treatments may provide therapeutic benefit", J Clin Aesthet Dermatol, 7, pp.20~25 (2014)
- Steinhoff M, "New insights into rosacea pathophysiology: a review of recent findings", J Am Acad Dermatol, 69, pp.S15~S26 (2013)
- Yamasaki K, "Increased serine protease activity and cathelicidin promotes skin inflammation in rosacea", Nat Med, 13, pp.975~980 (2007)
- Yamasaki K, "Kallikrein-mediated proteolysis regulates the antimicrobial effects of cathelicidins in skin", FASEB J, 20, pp.2068~2080 (2006)
- Morizane S, "Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands", J Invest Dermatol, 132, pp.135~143 (2012)
- Schauber J, "The vitamin D pathway: a new target for control of the skin's immune response?", Exp Dermatol, 17, pp.633~639 (2008)
- Pecze L, "Human keratinocytes are vanilloid resistant", PLoS One, 3, pp.e3419~ (2008)
- Caterina MJ, "The capsaicin receptor: a heat-activated ion channel in the pain pathway", Nature, 389, pp.816~824 (1997)
- Sulk M, "Distribution and expression of non-neuronal transient receptor potential (TRPV) ion channels in rosacea", J Invest Dermatol, 132, pp.1253~1262 (2012)
- Huggenberger R, "The cutaneous vascular system in chronic skin inflammation", J Investig Dermatol Symp Proc, 15, pp.24~32 (2011)
- Gomaa AH, "Lymphangiogenesis and angiogenesis in non-phymatous rosacea", J Cutan Pathol, 34, pp.748~753 (2007)
- Balato A, "IL-33 is secreted by psoriatic keratinocytes and induces pro-inflammatory cytokines via keratinocyte and mast cell activation", Exp Dermatol, 21, pp.892~894 (2012)
- Borelli C, "Dermasence refining gel modulates pathogenetic factors of rosacea in vitro", J Cosmet Dermatol, 16, pp.e31~e36 (2017)
- Denizot F, "Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability", J Immunol Methods, 89, pp.271~277 (1986)
- Schauber J, "Injury enhances TLR2 function and antimicrobial peptide expression through a vitamin D–dependent mechanism", J Clin Invest, 117, pp.803~811 (2007)
- Thibaut de Ménonville ST, "Topical treatment of rosacea with ivermectin inhibits gene expression of cathelicidin innate immune mediators, LL-37 and KLK5, in reconstructed and ex vivo skin models", Dermatol Ther, 7, pp.213~225 (2017)
- Lee JB, "Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin", Exp Dermatol, 25, pp.956~961 (2016)
- Morizane S, "Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid", J Invest Dermatol, 130, pp.1297~1306 (2010)
- Hertog MG, "Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study", Lancet, 342, pp.1007~1011 (1993)
- Vinson JA, "Phenol antioxidant quantity and quality in foods: fruits", J Acric Food Chem, 49, pp.5315~5321 (2001)
- 이설림, "2년 동안 지실의 채취시기 및 지역에 따른 Poncirin과 Naringin의 함량", 생약학회지, 42(2), pp.138~143 (2011), 한국생약학회
- 양희선, "유자즙을 첨가하여 제조한 막걸리의 발효기간 중 이화학적 특성 및 제조된 막걸리의 관능적 특성", 한국식품과학회지, 43(4), pp.438~445 (2011), 한국식품과학회
- Woo DH, "Stabilization to sunlight of natural coloring matter by soluble methyl-hesperidin", Korean J Food Sci Technol, 32, pp.50~55 (2000)
- 김성영, "Evaluation of Physiological Activities of the Citron (Citrus junos Sieb. ex TANAKA) Seed Extracts", Preventive Nutrition and Food Science, 18(3), pp.196~202 (2013), 한국식품영양과학회[DOI]
- 우강융, "HPLC 및 HPLC", MS를 이용한 유자 씨앗 중 플라보노이드 및 리모노이드 화합물의 분석, 2006, pp.35(3), 353~358[DOI]
- Manners GD, "Citrus limonoids: analysis, bioactivity, and biomedical prospects", J Agric Food Chem, 55, pp.8285~8294 (2007)
- Miller EG, "Inhibition of hamster buccal pouch carcinogenesis by limonin 17‐beta‐D‐glucopyranoside", Nutr Cancer, 17, pp.1~7 (1992)
- El-Readi MZ, "Inhibition of P-glycoprotein activity by limonin and other secondary metabolites from citrus species in human colon and leukaemia cell lines", Eur J Pharmacol, 626, pp.139~145 (2010)
- Langeswaran K, "Chemotherapeutic efficacy of limonin against Aflatoxin B1 induced primary hepatocarcinogenesis in Wistar albino rats", Biomed Aging Pathol, 2, pp.206~211 (2012)
- Giamperi L, "Antioxidant activity of citrus paradisi seeds glyceric extract", Fitoterapia, 75, pp.221~224 (2004)
- Koczulla R, "An angiogenic role for the human peptide antibiotic LL-37", hCAP-18, 2003, pp.111, 1665~1672
- Two AM, "Rosacea: part I. Introduction, categorization, histology, pathogenesis, and risk factors", J Am Acad Dermatol, 72, pp.749~758 (2015)
- Earley S, "Vanilloid and melastatin transient receptor potential channels in vascular smooth muscle", Microcirculation, 17, pp.237~249 (2010)
- Wang TT, "Cutting edge: 1,25-dihydroxyvitamin D3 is a direct inducer of antimicrobial peptide gene expression", J Immunol, 173, pp.2909~2912 (2004)
- Nilius B, "Transient receptor potential cation channels in disease", Physiol Rev, 87, pp.165~217 (2007)
