형질전환된 식물세포에서 Sucrose 농도가 hGM-CSF 생산에 미치는 영향
Effects of Sucrose Concentration on the Production of hGM-CSF in Transgenic Plant Cell Suspension Culture
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본 연구에서는 hGM-CSF 유전자가 도입된 형질전환 담배세포에서 sucrose의 농도가 배양 배지내 분비된 hGM- CSF, 총분비 단백질, 그리고 단백질 분해효소에 미치는 효과를 보았다. 10, 30, 60, 90, 120, 150 g/L의 sucrose 농도로 배양한 결과 sucrose농도가 30 g/L일 때 건조중량은 11.22 g/L, 분비된 hGM-CSF의 양은 181.53 ㎍/L, 그리고 총단백질은 66.8 mg/L로 시료채취 5일 때 가장 높은 값을 나타내었고, 단백질 분해효소는 sucrose농도가 120 g/L일 때 2660 U/L의 값을 나타내었다. 그러나 배양 10일째 sucrose농도 60 g/L에서 건조중량이 28.36 g/L로 가장 높은 값을 나타낸 반면 분비된 hGM-CSF의 최대 값은 95 ㎍/L로 sucrose농도가 150 g/L일 때 가장 높은 값을 나타내었다.
Abstract
The effects of sucrose concentration on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.22 g/L), hGM-CSF (181.53 ㎍/L) and total protein (66.8 mg/L) were detected as highest at 30 g/L sucrose and protease activity (2660 U/L) was highest at 120 g/L sucrose after 5-day culture. However after 10-day culture, the maximum dry cell weight (28.36 g/L) was found at 60 g/L sucrose while the maximum hGM-CSF (95 ㎍/L) was appeared at 150 g/L sucrose. The total protein and protease activity was secreted as 52.28 mg/L and 3430 U/L, respectively in the same culture.
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