Zinc finger RING-H2 protein관련 Ac/Ds전이인자 삽입 변이체 Oszinc626 유전자의 특성 분석
Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice (Oryza sativar L.)
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Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless (Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626 (NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2 (C3-H2-C3 type) domain for this group of protein is Cys-X2-Cys-X28-Cys-X-His-X2-His-X2-Cys-X14-Cys-X2-Cys. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.
Abstract
Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless (Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626 (NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2 (C3-H2-C3 type) domain for this group of protein is Cys-X2-Cys-X28-Cys-X-His-X2-His-X2-Cys-X14-Cys-X2-Cys. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.
참고문헌 (39)
- Borden K. L. B., "The RING finger domain: a recent example of a sequence-structure family", Curr Opin Struct Biol, 6, pp.395~401 (1996)
- Chaudhury A. M., "Control of early seed development", Annu Rev Cell Dev Biol, 17, pp.677~699 (2001)
- Deng X. W., "COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain", Cell, 71, pp.791~801 (1992)
- Freemont P. S., "A novel cysteinerich sequence motif", Cell, 64, pp.483~484 (1991)
- Freemont P. S., "Ubiquitination: RING for destruction?", Curr Biol, 10, pp.R84~R87 (2000)
- Goldberg R. B., "Plant embryogenesis: zygote to seed", Science, 266, pp.605~614 (1994)
- Han C. D., "Molecular Analysis of Rice Plants Harboring an Ac", Ds Transposable Element-Mediated Gene Trapping System, 1999, pp.19, 615~624
- Han C. D., "Rice functional genomics by transposon mutagenesis", Asia Pacific Biotech News, 6, pp.930~935 (2002)
- Hayashi H., "Phytohormone-independent division of tobacco protoplast- derived cells", Science, 258, pp.1350~3 (1992)
- Hiei Y., "Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA", Plant J, 6, pp.271~282 (1994)
- Himmelbach A., "Relay and control of abscisic acid signaling", Curr Opin Plant Biol, 6, pp.470~479 (2003)
- Jensen R. B., "Widespread occurrence of a highly conserved RING-H2 zinc finger motif in the model plant Arabidopsis thaliana", FEBS Lett, 436, pp.283~287 (1998)
- Jefferson R. A., "Assaying chimeric genes in plants: the GUS gene fusion system", Plant Mol. Biol. Rep, 5, pp.387~405 (1987)
- Kim C. M., "Reprogramming of the activity of Ac", Ds transposon family during plant regeneration in rice, 2002, pp.14, 231~237
- Kim C. M., "Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice", Plant J, 39, pp.252~263 (2004)
- Knight H., "Calcium signaling in Arabidopsis thaliana responding to drought and salinity", Plant J, 12, pp.1067~1078 (1997)
- Lechner E., "A gene trap Dissociation insertion line, associated with a RING-H2 finger gene, shows tissue specific and developmental regulated expression of gene in Arabidopsis", Gene, 290, pp.63~71 (2002)
- Martínez-García M., "Gene isolation in Arabidopsis thaliana by conditional overexpression of cDNAs toxic to Saccharomyces cerevisiae: identification of a novel early response zinc-finger gene", Mol Gen Genet, 252, pp.587~596 (1996)
- McNellis T. W., "Overexpression of Arabidopsis COP1 results in partial suppression of light-mediated development: evidence for a lightinactivable repressor of photomorphogenesis", Plant Cell, 6, pp.1391~400 (1994)
- Meinke D. W., "Arabidopsis in : Seed developement in Arabidopsis thaliana.", pp.253~295 (1994), Cold Spring Harbor Laboratory Press
- Meinke D. W., "Molecular genetics of plant embryogenesis. Annu Rev Plant Physiol", Plant Mol Biol, 46, pp.369~394 (1995)
- Mendoza I., "The protein phosphatase calcineurin is essential for NaCl tolerance of Saccararomyces cerevisiae", J Biol Chem, 269, pp.8792~8796 (1994)
- Molnár G., "Characterization of BRH1, a brassinosteroid-responsive RING-H2 gene from Arabidopsis thaliana", Planta, 215, pp.127~133 (2002)
- Park S. J., "Analysis of Intragenic Ds Transpositions and Excision Events Generating Novel Allelic Variation in Rice", Mol Cells, 21, pp.284~293 (2006)
- Park S. H., "A Ds-insertion mutant of OSH6 (Oriza sativa homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice", Planta, 227, pp.1~12 (2007)
- Rashid H., "Transgenic plant production mediated by Agrobacterium in Indica rice", Plant Cell Rep, 15, pp.727~730 (1996)
- Ruqiang X., "A RING-H2 zinc-finger protein gene RIE1 is essential for seed development in Arabidopsis", Plant molecular biology, 53, pp.27~50 (2003)
- Saurin A. J., "Does this have a familiar RING?", Trends Biochem Sci, 21, pp.208~214 (1996)
- Stone S. L., "Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis", Plant Physiol, 137, pp.13~30 (2005)
- Takayama KM, "Antisense RNA", Cut Rev Biochem molec Biol, 25, pp.155,306~17 (1990)
- Thomas T. L., "Gene expression during plant embryogenesis and germination: an overview", Plant Cell, 5, pp.1401~1410 (1993)
- Wayne F., "Retroviral insertional mutagenesis of a target allele in a heterozygous murine cell line", Proc Nati Acad Sci USA, 통권 : 82, pp.6600~6604 (1985)
- Wilkins M. R., "Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it", Biotechnol Genet Eng Rev, 13, pp.19~50 (1996)
- Xiong L., "Regulation of abscisic acid biosynthesis", Plant Physiol, 133, pp.29~36 (2003)
- Xiuhong Y., "Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum", J Biosci, 33, pp.103~112 (2008)
- Yoshida S., "Laboratory manual for physiological studies of rice. Ed3" (1976), The International Rice Research Institute Press
- Yun D. J., "Molecular mechanism of plant adaption to high salinity", Korean J Plant Biotechnol, 32, pp.1~14 (2005)
- Zhu J. K., "Salt and drought stress signal transduction in plant", Annu Tev Plant Biol, 53, pp.247~273 (2002)
- Zou J., "Cloning and molecular characterization of an Arabidopsis thaliana RING zinc finger gene expressed preferentially during seed development", Gene, 196, pp.291~295 (1997)