멸종위기 식물 덩굴용담의 기내번식에 미치는 생장조절제 효과
Effect of plant growth regulators on micropropagation of a rare and endangered species, Tsuru-rindo (Tripterospermum japonicum)
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초록
멸종위기 식물 덩굴용담의 기내증식법 개발을 위하여 기내증식 에 미치는 생장조절제의 효과를 시험하였다. 6가지의 기본배지 시 험에서 MS 배지가 줄기증식에 가장 적합한 것으로 나타났다. 싸이 토키닌 가운데 BA 처리는 3.0 mg/L 농도까지 증식에 유의적인 증 식효과를 보였고, 그 이상 농도에서는 증식이 억제되었다. Kinetin 과 TDZ는 BA 만큼 증식에 효과적이지 못했다. 발근에 있어 TDZ 는 저농도에서도 발근을 현저히 억제하였으나, BA와 kinetin은 저 농도 처리 조건에서 자발적인 발근이 이루어 졌다. 한편 지베렐릭 산 (GA3)의 처리는 줄기신장의 유의적인 효과가 있었다. 증식중인 줄기는 인공상토에 기외삽목하여 90% 이상 발근이 가능하고 정상 적인 생장이 이루어졌다. 대부분의 식물체는 토양이식 다음해에 개화되고 과실을 맺었다.
Abstract
Various plant growth regulators were tested for shoot proliferation of Tripterospermum japonicum, a rare and endangered species. Among the six different media tested, MS medium was the best for the shoot growth. Whereas BA, upto 3 mg/L, significantly increased shoot proliferation rate, it suppressed the rate at higher levels. Neither kinetin nor TDZ was so effective in proliferating shoots as BA. As for rooting, TDZ strongly inhibited it even at very low concentration though spontaneous rooting was frequently observed from the proliferated shoots during culture of lower concentration BA or kinetin. In contrast, shoot elongation was significantly promoted by GA3. More than 90% of the proliferated plantlets could be transplanted via cuttings into pots containing artificial soil mixture where they rooted and resumed normal growth. Most of the plants bloomed to bear fruits in the following year.
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