고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향
Influence of pretreatment medium, fresh medium addition, and culture plate size on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.)
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고추의 소포자 배양 시 전처리 배지, 배양 후 새 배지의 첨가, 및 배양 용기의 크기가 배의 발생 및 발달에 미치는 영향을 조사하 였다. 고온 전처리 (32±1℃) 시 사용된 배지 중 배의 생산에 적합한 것은 당-기아배지 A이었으며, 0.37 M mannitol 용액이나 sucrose가 첨가된 NLNS배지는 배의 생산에 비효율적이었다. 전처리 배지인 당-기아배지 A와 0.37 M mannitol 용액에 배양배지를 첨가하는 경 우 배의 발달 정도는 증가하였으나 배의 생산은 크게 감소하였다. 배양 2, 3 주 후의 새 배지 첨가는 배의 생산에 효과가 없었다. 배 양용기는 3.5×1.0 cm 크기의 것 보다는 6.0×1.5 cm 크기의 것을 사 용하는 것이 배의 발달에 효과적이었다. 이상에서와 같은 결과들 은 고추에서 다수의 배를 생산할 수 있는 소포자 배양시스템을 확 립하는데 중요한 기초자료가 될 것이다.
Abstract
The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment (32±1℃), high frequency embryo production was obtained when the sucrose- starvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from 3.5×1.0 cm to 6.0×1.5 cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.
참고문헌 (48)
- Baillie AMR, "In vitro culture of isolated microspores and regeneration of plants in Brassica campestris", Plant Cell Rep, 11, pp.234~237 (1992)
- Biddington NL, "Ethylene production during anther culture of Brussels sprout (Brassica oleracea var. gemmifera) and its relationship with factors that affect embryo production", Plant Cell Tissue Organ Cult, 25, pp.169~177 (1991)
- Buyukalaca S, "Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture", Eur J Hort Sci, 69(5), pp.206~209 (2004)
- Castillo AM, "Efficient production of androgenic doubled-haploid mutants in barley by the application of sodium azide to anther and microspore cultures", Plant Cell Rep, 20, pp.105~111 (2001)
- Dunwell JM, "Anther culture in Nicotiana tabacum. The role of the culture vessel atmosphere in pollen embryo induction and growth", J Exp Bot, 30, pp.419~428 (1979)
- Dunwell JM, "Role of sucrose in microspore embryo production in Brassica napus ssp. oleifera", J Exp Bot, 36, pp.1478~1491 (1985)
- Ferrie AMR, "Evaluation of Brassica napus L. genotypes for microspore culture response and identification of a highly embryogenic line", Plant Cell Rep, 14, pp.580~584 (1995)
- Ferrie AMA, "Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae", In Vitro Cell Dev Biol, 43, pp.348~355 (2007)
- Hansen M, "Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media", Plant Cell Reports, 12, pp.496~500 (1993)
- Heslop-Harrison JS, "Cytological techniques to assess pollen quality. In Sexual plant repreduction", pp.41~48 (1970), Springer-Verlag
- Hoekstra S, "Androgenesis in Hordeum vulgareL.: Effects of mannitol, calcium and abscisic acid on anther pretreatment", Plant Sci, 126, pp.211~218 (1997)
- Hoekstra S, "Anther and microspore culture of Hordeum vulgare L. cv Igri", Plant Sci, 86, pp.89~96 (1992)
- Höfer M, "Induction of embryogenesis from isolated apple microspores", Plant Cell Rep, 18, pp.1012~1017 (1999)
- Hu T, "Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture", Plant Cell Rep, 16, pp.520~525 (1997)
- Hu TC, "Isolated microspore culture of wheat (Triticum estivum L.) in a defined media I. effects of pretreatment, isolation methods, and hormones", In Vitro Cell Dev Biol, 31, pp.79~83 (1995)
- Immonen S, "Media composition and anther plating for production of androgenetic green plants from cultivated rye (Secale cereale L.)", J Plant Physiol, 156, pp.204~210 (2000)
- Jähne A, "Regeneration of transgenic, microspore-derived, fertile barley", Theor Appl Genet, 89, pp.525~533 (1994)
- Jähne A, "Cereal microspore culture", Plant Sci, 109, pp.1~12 (1995)
- Kasha KJ, "Haploids in creal improvement: anther and microspore culture. In Gene manipulation in plant improvement II", pp.213~235 (1990), Plenum
- Kernan Z, "Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria)", Plant Cell Rep, 25, pp.274~28 (2006)
- Kim M, "Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture", Plant Cell Rep, 27, pp.425~434 (2008)
- Kim M, "Increased emrbryo production by manipulation of plantreatment materials and media in isolated microspore culture of hot pepper (Capsicum annuum L.). In Kumar A, eds, Recent advances in plant biotechnology", pp.572~588 (2009), The India
- Kott LS, "Autotoxicity in isolated microspore cultures of Brassica napus", Can J Bot, 66, pp.1665~1670 (1988)
- Kyo M, "Studies on conditions for cell division and embryogenesis in isolated pollen culture of Nicotiana rustica", Plant Physiol, 79, pp.90~94 (1985)
- Kyo M, "Control of the developmental pathway of tobacco pollen in vitro", Planta, 168, pp.427~432 (1986)
- 김문자, "고추의 소포자 배양 시 모식물의 생육조건, 소포자 나출 방법, 치상배지 및 광배양이 배의 발생에 미치는 영향", Journal of Plant Biotechnology, 34(4), pp.363~373 (2007), 한국식물생명공학회
- Li H, "Enhancement of microspore culture efficiency of recalcitrant barley genotypes", Plant Cell Rep, 20, pp.475~481 (2001)
- Lichter R, "Induction of haploid plants from isolated pollen of Brassica napus. Z", Pflanzenphysiol, 105, pp.427~434 (1982)
- Lionneton E, "Improved microspore culture and doubled-haploid plant regeneration in the brown condiment mustard (Brassica juncea)", Plant Cell Rep, 20, pp.126~130 (2001)
- Liu W, "Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.)", Plant Cell Rep, 20, pp.821~824 (2002)
- Mitykó J, "Problems and results of doubled haploid plant production in pepper (Capsicum annuum L.) via antherand microspre culture", Acta Hort, 447, pp.281~287 (1997)
- Nägeli M, "Improved formation of regenerable callus in isolated microspore culture of maize: impact of carbohydrates, plating density and time of transfer", Plant Cell Rep, 19, pp.177~184 (1999)
- Oleszczuk S, "Androgenic response to preculture stress in microspore cultures of barley", Protoplasma, 228, pp.95~100 (2006)
- 박은준, "고추의 나출 소포자 배양 시 전처리 기간, 2-Hydroxynicotinic Acid 및 약-공동전처리가 소포자배 발생에 미치는 영향", Journal of Plant Biotechnology, 32(1), pp.37~44 (2005), 한국식��생명공학회
- Raina SK, "High-frequency embryogenesis and plantlet regeneration from isolated microspores of indica rice", Plant Cell Rep, 17, pp.957~962 (1998)
- Regner F, "Anther and microspore culture in Capsicum, In In vitro haploid production in higher plants, vol 3", pp.77~89 (1996), Kluwer
- Stöger E, "Plant transformation by particle bombardment of embryogenic pollen", Plant Cell Rep, 14, pp.273~278 (1995)
- Supena EDJ, "Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.)", Plant Cell Rep, 25, pp.1~10 (2006)
- Swanson EB, "Microspore culture in Brassica : In Methods in molecular biology, Vol. 6, Plant cell and tissue culture.", pp.159~170 (1990), Humana Press
- Swanson EB, "Efficient isolation of microspores and the production of microspore-derived embryos from Brassica napus", Plant Cell Reports, 6, pp.94~97 (1987)
- Swanson EB, "Microspore mutagenesis and selection: canola plants with field tolerance to the imidazolinones", Theor Appl Genet, 78, pp.525~531 (1989)
- Testillano PS, "The immunolocalization of nuclear antigens during the pollen developmental program and the induction of pollen embryogenesis", Exp Cell Res, 221, pp.41~54 (1995)
- Testillano PS, "Differentiating plant cells switched to proliferation remodel the functional organization of nuclear domains", Cytogenet Genome Res, 109, pp.166~174 (2005)
- Touraev A, "Stressinduced microspore embryogenesis in tobacco: an optimized system for molecular studies", Pant Cell Rep, 15, pp.561~565 (1996)
- Touraev A, "Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature", Sex Plant Reprod, 9, pp.209~215 (1996)
- Touraev A, "Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of microspore", pollen embryogenesis, 1996, pp.200, 144~152
- van Bergen S, "The role of abscisic acid in induction of androgenesis: A comparative study between Hordeum vulgare L. Cvs. Igri and Digger", J Plant Growth Regul, 18, pp.135~143 (1999)
- Wang M, "Apoptosis in developing anthers and the role of ABA in this process during androgenesis in Hordeum vulgare L", Plant Molecular Biology, 39, pp.489~501 (1999)
