조건불리지역 재배를 위한 산화스트레스 내성 고구마 품종의 선발
Selection of oxidative stress-tolerant sweetpotato cultivars for cultivation on marginal lands
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초록
식물이 환경스트레스에 노출되게 되면 과다한 활성산 소의 축적으로 인한 산화스트레스가 발생한다. 그러므로 재해내성 작물개발을 위한 분자육종의 재료로써 스트레 스 내성 고구마 품종을 선발하기 위해, 본 연구에서는 10 종의 고구마의 잎 disc에 산화스트레스 유도원인 methyl viologen (MV)을 처리하였다. MV 처리 후 각 고구마 품종의 이온전도도는 DPPH radical 소거활성과 높은 상관관계를 나타내었다. 본 연구 에서 낮은 이온전도도 값과 높은 DPPH radical 소거활성 및 전체 페놀함량을 나타낸 Yanshu 8, 신황미, 신자미 세 품종을 산화스트레스 내성 품종으로 선발할 수 있었다. 본 연구의 결과를 통해 DPPH radical 소거활성이 복합재 해내성 작물선발을 위한 좋은 실험방법임을 알 수 있었 으며, 산화스트레스 내성 고구마 품종들이 보인 MV에 대한 내성은 증가된 항산화 활성과 연관된 것임을 알 수 있었다.
Abstract
Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.
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