엽록체 형질전환 유래 분자 농업의 연구 동향
Current status on plant molecular farming via chloroplast transformation
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참고문헌
초록
고등식물의 엽록체 형질전환은 핵 형질전환에서 기대할 수 없는 여러 가지 이점을 가진다. 외래 단백질의 발현율을 획기적으로 높일 수 있고, 여러 유전자를 동시에발현시킬 수 있으며, 상동재조합에 의한 부위-특이적 유전자 삽입으로 인해 유전자 침묵 및 위치효과가 없다. 더욱이, 대부분 작물은 화분을 통한 도입된 유전자의 전이가 불가능한 모계 유전을 하기 때문에 엽록체 형질전환은 환경 친화적이다. 엽록체 형질전환 시스템은 핵 형질전환과 달리 작물에서의 성공에 제한적이었으나 지난 10년 동안 이런 한계가 극복되어 콩, 당근, 상추 및 유채 등의 작물에서도 성공하게 되었다. 그러므로 이제 작물의엽록체 형질전환은 농업적 형질의 개선뿐 만 아니라, 고부가가치 백신과 의료용 단백질 생산을 통한 의약품 산업의 성장에 활용될 수 있을 것이다.
Abstract
Chloroplast transformation in higher plants offers many attractive advantages over nuclear transformation, including a high-level accumulation of foreign proteins, multi-gene expression in single transformation event via transgene stacking in operons and no position effect due to site-specific integration of transgenes by homologous recombination. Most importantly, chloroplast transgenic plants are eco-friendly because their transgenes are maternally inheritance in most crop plants. However, chloroplast transformation system has limited success in crops alike nuclear transformation. In the past two decades, great progress has been made to overcome the limitations of chloroplast transformation, thus expending chloroplast bioreactor to several important crops including soybean, carrot, lettuce, and oilseed. Therefore, it has become possible that chloroplast transformation of crops can be used not only for the improvement of agronomic traits, but also for the production of vaccines and high valuable therapeutic proteins in pharmaceutical industry.
참고문헌 (62)
- Arlen PA, "Field production and functional evaluation of chloroplast-derived interferon-alpha2b", Plant Biotechnol J, 5, pp.511~525 (2007)
- Arlen PA, "Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts", Infect Immun, 76, pp.3640~3650 (2008)
- Bendich AJ, "Why do chloroplasts and mitochondria contain so many copies of their genome?", BioEssays, 6, pp.279~282 (1987)
- Birch-Machin I, "Accumulation of ritavirus VP6 protein in chloroplasts of transplastomic tobacco is limited by protein stability", Plant Biotechnol J, 2, pp.261~270 (2004)
- Boynton JE, "Chloroplast transformation in Chlamydomonas with high velocity microprojectiles", Science, 240, pp.1534~1538 (1988)
- Carrer H, "Kanamycin resistance as a selectable marker for plastid transformation in tobacco", Mol Gen Genet, 241, pp.49~56 (1993)
- Chakrabarti S, "Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers resistance to potato tuber moth", Transgenic Res, 15, pp.481~488 (2006)
- Chebolu S, "Chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality. in: Plant-produced microbial vaccines. Current Topics in Microbiology and Immunology 332", pp.33~54 (2009), Springer-Verlag Berlin Heidelberg Germany
- Chung HJ, "Chloroplast genetic transformation in higher plants: an encounter between prokaryote and eukaryote", J Plant Biotechnol, 33, pp.185~194 (2006)
- Daniell H, "Molecular strategies for gene containment in transgenic crops", Nat Biotechnol, 20, pp.581~586 (2002)
- Daniell H, "Transgene containment by maternal inheritance:effective or elusive?", Proc Natl Acad Sci USA, 통권 : 104, pp.6879~6880 (2007)
- Daniell H, "Containment of herbicide resistance through genetic engineering of the chloroplast genome", Nat Biotechnol, 16, pp.345~348 (1998)
- Daniell H, "Chloroplast derived antibodies, biopharmaceuticals and edible vaccines. in: Molecular Farming", pp.113~133 (2004), Wiley-VCH Verlag
- Daniell H, "Chloroplast-derived vaccine antigens and other therapeutic proteins", Vaccine, 23, pp.1779~1783 (2005)
- Daniell H, "Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts", J Mol Biol, 311, pp.1001~1009 (2001)
- Daniell H, "Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection", Curr Genet, 39, pp.109~116 (2001)
- Seyed Javad Davarpanah, "Stable Plastid Transformation in Nicotiana benthamiana", Journal of Plant Biology(한국식물학회지), 52(3), pp.244~250 (2009), 한국식물학회
- De Cosa B, "Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals", Nat Biotechnol, 19, pp.71~74 (2001)
- DeGray G, "Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi", Plant Physiol, 127, pp.852~862 (2001)
- Dufourmantel N, "Generation of fertile transplastomic soybean", Plant Mol Biol, 55, pp.479~489 (2004)
- Eibl C, "In vivo analysis of plastid psbA, rbcL and rpl32 UTR elements by chloroplast transformation: Tobacco plastid gene expression is controlled by modulation of transcript levels and translation efficiency", Plant J, 19, pp.333~345 (1999)
- Elderbaum O, "Expression of active human interferon beta in transgenic plants", J Interferon Res, 12, pp.449~453 (1992)
- Fernandez-San Millan A, "A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation", Plant Biotechnol J, 1, pp.71~79 (2003)
- Gruissem W, "Control mechanisms of plastid gene expression", CRC Crit Rev Plant Sci, 21, pp.19~55 (1993)
- Guda C, "Stable expression of biodegradable protein based polymer in tobacco chloroplasts", Plant Cell Rep, 19, pp.257~262 (2000)
- Hagemann R, "The sexual inheritance of plant organelles. in: Molecular Biology and Biotechnology of Plant Organelles", pp.93~113 (2004), Springer
- Hou BK, "Chloroplast transformation in oilseed rape", Transgenic Res, 12, pp.111~114 (2003)
- Iamtham S, "Removal of antibiotic resistance genes from transgenic tobacco plastids", Nat Biotechnol, 18, pp.1172~1176 (2000)
- Jeong SW, "Dicistronic expression of the green fluorescent protein and antibiotic resistance genes in the plastid for selection and tracking of plastid-transformed cells in tobacco", Plant Cell Rep, 22, pp.747~751 (2004)
- Khan MS, "Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants", Nat Biotechnol, 17, pp.910~915 (1999)
- Kittiwongwattana C, "Plastid marker gene excision by the phiC31 phage site-specific recombinase", Plant Mol Biol, 64, pp.137~143 (2007)
- Ko SM, "Production of human serum albumin in chloroplast-transformed tobacco plants", J Plant Biotechnol, 33, pp.233~236 (2006)
- Kota M, "Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects", Proc Natl Acad Sci USA, 통권 : 96, pp.1840~1845 (1999)
- Koya V, "Plant based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge", Infect Immun, 73, pp.8266~8274 (2005)
- Kumar S, "Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance", Plant Physiol, 136, pp.2843~2854 (2004)
- Lee SB, "Accumulation of trehalose within transgenic chloroplasts confers drought tolerance", Mol Breed, 11, pp.1~13 (2003)
- Leelavathi S, "Chloroplast expression of Histagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors", Mol Breed, 11, pp.49~58 (2003)
- Liu CW, "Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment", Plant Cell Rep, 26, pp.1733~1744 (2007)
- Liu JR, "Plastid transformation system to prevent the intramolecular recombination of transgene. PCT", 2006, pp.2006, ~
- Maliga P, "Plastid transformation in higher plants", Annu Rev Plant Biol, 55, pp.289~313 (2004)
- McBride KE, "Amplification of a chimeric Bacillus gene in chloro plasts leads to an extraordinary level of an insecticidal protein in tobacco", Biotechnology (NY), 13, pp.362~365 (1995)
- Molina S, "High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts", Plant Biotechnol J, 2, pp.141~153 (2004)
- Molina S, "Induction of neutralizing antibodies by a tobacco chloroplast-derived vaccine based on a B cell epitope from canine parvovirus", Virology, 342, pp.266~275 (2005)
- Okumura S, "Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts", Transgenic Res, 15, pp.637~646 (2006)
- Ruf S, "Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit", Nat Biotechnol, 19, pp.870~875 (2001)
- Ruhlman T, "Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplast-oral administration protects against development of insulitis in non-obese diabetic mice", Plant Biotechnol J, 5, pp.495~510 (2007)
- Ruiz ON, "Engineering cytoplasmic male sterility via the chloroplast genome by expression of β-ketothiolase", Plant Physiol, 138, pp.1232~1246 (2005)
- Ruiz ON, "Phytoremediation of organomercurial compounds via chloroplast genetic engineering", Plant Physiol, 132, pp.1344~1352 (2003)
- Sidorov VA, "Stable chloroplast transformation in potato:use of green fluorescent protein as a plastid marker", Plant J, 19, pp.209~216 (1999)
- Sikdar SR, "Plastid transformation in Arabidopsis thaliana", Plant Cell Rep, 18, pp.20~24 (1998)
- Singh AK, "Plastid transformation in eggplant (Solanum melongena L.)", Transgenic Res, 19, pp.113~119 (2010)
- Staub JM, "High-yield production of a human therapeutic protein in tobacco chloroplasts", Nat Biotechnol, 18, pp.333~338 (2000)
- Svab Z, "Stable transformation of plastids in higher plants", Proc Natl Acad Sci USA, 통권 : 87, pp.8526~8530 (1990)
- Svab Z, "High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene", Proc Natl Acad Sci USA, 통권 : 90, pp.913~917 (1993)
- Torrado J, "Pharmacological characteristics of parenteral IGF-1 administration", Curr Pharm Biotechnol, 4, pp.123~140 (2003)
- Tregoning JS, "Expression of tetanus toxin fragment C in tobacco chloroplasts", Nucleic Acids Res, 31, pp.1174~1179 (2003)
- Verma D, "Chloroplast vector systems for biotechnology applications", Plant Physiol, 145, pp.1129~1143 (2007)
- Verma D, "A protocol for expression of foreign genes in chloroplasts", Nat Protocol, 3, pp.739~758 (2008)
- Wang HH, "Advances in chloroplast engineering", J Genet Genomics, 36, pp.387~398 (2009)
- Watson J, "Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food", feed crop, 2004, pp.22, 4374~4384
- Youm JW, "High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice", Transgenic Res, 10 (2010)
- Zubko MK, "Stable transformation of petunia plastids", Transgenic Res, 13, pp.523~530 (2004)
