Next generation sequencing 방법을 이용한 적육계 복숭아와 백육계 복숭아의 전사체 분석
Comparison of transcriptome analysis between red flash peach cultivar and white flash peach cultivar using next generation sequencing
2
조회
0
다운로드
2
인용
0
참고문헌
초록
Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress,ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.
Abstract
Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress,ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.
참고문헌 (32)
- Ahmad R, "Whole genome sequencing of peach (Prunus Persica L.) for SNP identification and selection" (2011)
- Altschul SF, "Basic local alignment search tool", J Mol Biol, 215, pp.403~410 (1990)
- Ashburner M, "Gene ontology: tool for the unification of biology. The Gene Ontology Consortium", Nature Genet, 25(1), pp.25~29 (2000)
- Butler WL, "Effect of divalent cations on energy coupling between the light harvesting chlorophyll a", b comples and photosystem II, 1978, 9~20[DOI]
- Carolina Di Santo M, "Differential expression of alpha-1-arabinofuranosidase and alpha-1- arabinofuranosidase", beta-d-xylosidase genes during peach growth and ripening, 2005, pp.47(7), 562~569
- Childers NF, "The peach", pp.1~8 (1988), Somerset press
- Drexler HC, "Activation of the cell death program by inhibition of proteasome function", Proc Natl Acad Sci USA, pp.855~860 (1997)
- Dunsmuir P, "The petunia chlorophyll a", b binding protein genes: a comparison of Cab genes from different gene families, 1985, pp.13, 2503~2518
- Forkmann G, "Metabolic engineering and applications of flavonoids", Curr Opin in Biotech, 12, pp.155~160 (2001)
- Fraser PD, "Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner", Proc Natl Acad Sci USA, 99, pp.1092~1097 (2002)
- Goffreds JC, "Inheritance of isozymes in peach x Prunus Kansuensisand peach x Prunus davidiana hybrids", 54, pp.161~168 (1991)
- Hanana M, "Identification and characterization of “rd22” dehydration responsive gene in grapevine (Vitis vinifera L.)", C R Biol, 331(8), pp.569~578 (2008)
- Holton TA, "Genetics and biochemistry of anthocyanin biosynthesis", Plant Cell, 7, pp.1071~1083 (1995)
- Hui-Hsien C, "DNA sequence quality trimming and vector removal", Bioinformatics, 17(12), pp.1093~1104 (2001)
- Kristin B, "Mutations in soybean microsomal omega-3-fatty acid desaturase genes reduce linolenic acid concentration in soybean seeds", Crop Science, 45(5), pp.1830~1836 (2005)
- Lester DR, "Peach (Prunus persica) endopolygalacturonase cDNA isolation and mRNA analysis in melting and nonmelting peach cultivars", Plant Physiol, 105(1), pp.225~231 (1994)
- Margulies M, "Genome sequencing in microfabricated high-density picolitre reactors", Nature, 437, pp.376~380 (2005)
- Martin S, "Mechanisms of mono-and polyubiquitination: Ubiquitination specificity depends on compatibility between the E2 catalytic core and amino acid residues proximal to the lysine", Cell Division, 5, pp.19~23 (2010)
- Mayfidld SP, "Expression of the nuclear encoding oxygen-evolving enhancer 2 is required for high levels of photosynthetic oxygen evolution in Chlamydomonas reinharditii", Proc Natl Acad Sci USA, 84(3), pp.749~753 (1987)
- Ogundiwin EA, "Leucoanthocyanidin dioxygenase gene (PpLDOX): a potential functional marker for cold storage browning in peach", Tree Genetics & Genomes, 4(3), pp.543~554 (2008)
- 박종석, "Flavonoid 생합성: 생화학과 대사공학적 응용", Journal of Plant Biotechnology, 29(4), pp.265~275 (2002), 한국식물생명공학회
- Peng Y, "Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants", Planta, 226, pp.729~740 (2007)
- Pozzi C, "Peach structural genomics, In Genetics and genomics of Rosaceae", pp.235~257 (2009), Springer
- Raffaella G, "Mechanisms of cell protection by heme oxygenase-1", Annu Rev Pharmacol Tosicol, 50, pp.323~354 (2010)
- Shujun C, "A simple and efficient method for isolating RNA from pine trees", Plant Molecular Biology Reporter, 11(2), pp.113~116 (1993)
- Thornber JP, "Chlorophyll-proteins: light-harvesting and reaction centre components of plants", Annu Rev Plant Physiol, 26, pp.127~155 (1975)
- Vidal JR, "Highefficiency biolistic co-transformation and regeneration of ‘Chardonnay’ (Vitis vinifera L.) containing npt-II and antimicrobial peptide genes", Plant Cell Rep, 22, pp.252~260 (2003)
- Walling LL, "Isolation, characterization and evolutionary relatedness of three members from the soybean multigene family endoding chlorophyll a", b binding proteins Nuc, 1988, pp.16, 10477~10492
- Wurgler-Murphy SM, "Two-component signal transducers and MAPK cascades", Trends Biochem Sci, 22, pp.172~176 (1997)
- Ying Z, "Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica F. atropurpurea)" (2012)
- Zhang M, "Cloning and functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits", J Plant Physol, 166(12), pp.1241~1252 (2009)
- Zhu C, "Differential responses of maize MIP genes to salt stress and ABA", J Exp Bot, 56, pp.2971~2981 (2005)