벼 유전자원의 수당립수 증진 유전자 유전형 분석
Genotype analysis of genes involved in increasing grain number per panicle in rice germplasm
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
ARice is an important staple food in the world and rice yield is one of the main traits for rice breeding. Several genes involved in increasing the yield have been identified through map-based gene cloning within natural variations in rice. These identified genes are good targets for introducing a genetic trait in molecular breeding. Here, we chose five genes reported to be involved in increasing grain number per panicle in rice; Gn1a, dep1, Apo1, Ghd7, and Nal1. We developed In/Del markers for Gn1a, and dep1, Apo1, and applied the reported SNP markers for Ghd7 and Nal1. We were easily able to examine the genotype of each gene on agarose gel. We tested the genotypes on 479 rice resources that we held with evaluated molecular markers. According to the genotype of each gene, rice resources were divided into 13 haplotypes, and most of the Indica and Japonica varieties were included in haplotypes 1 and 13, respectively. When we examined the effect of each gene on grain number per panicle and panicle number per plant, panicle number per plant in the yield negative allele group for each gene was reduced by approximately 0.3 to 0.8 compared to that in the yield positive allele group. However, the number of yield positive alleles for each gene was higher by about 21 to 27 grains per panicle than that of yield negative alleles. Although most of the varieties were grouped in haplotypes 1 and 13, we believe that this genotype information with evaluated molecular markers will be useful in rice breeding for increasing the yield with grain number per panicle.
Abstract
ARice is an important staple food in the world and rice yield is one of the main traits for rice breeding. Several genes involved in increasing the yield have been identified through map-based gene cloning within natural variations in rice. These identified genes are good targets for introducing a genetic trait in molecular breeding. Here, we chose five genes reported to be involved in increasing grain number per panicle in rice; Gn1a, dep1, Apo1, Ghd7, and Nal1. We developed In/Del markers for Gn1a, and dep1, Apo1, and applied the reported SNP markers for Ghd7 and Nal1. We were easily able to examine the genotype of each gene on agarose gel. We tested the genotypes on 479 rice resources that we held with evaluated molecular markers. According to the genotype of each gene, rice resources were divided into 13 haplotypes, and most of the Indica and Japonica varieties were included in haplotypes 1 and 13, respectively. When we examined the effect of each gene on grain number per panicle and panicle number per plant, panicle number per plant in the yield negative allele group for each gene was reduced by approximately 0.3 to 0.8 compared to that in the yield positive allele group. However, the number of yield positive alleles for each gene was higher by about 21 to 27 grains per panicle than that of yield negative alleles. Although most of the varieties were grouped in haplotypes 1 and 13, we believe that this genotype information with evaluated molecular markers will be useful in rice breeding for increasing the yield with grain number per panicle.
참고문헌 (24)
- Ashikari M, "Cytokinin oxidase regulates rice grain production", Science, 309, pp.741~745 (2005)
- Fujita D, "NAL1 allele from a rice landrace greatly increases yield in modern indica cultivars", Proc Natl Acad Sci USA, 110, pp.20431~20436 (2013)
- Hu J, "Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice", Rice (NY), 9, pp.30~ (2016)
- Huang X, "Natural variation at the DEP1 locus enhances grain yield in rice", Nat Genet, 41, pp.494~497 (2009)
- Ikeda-Kawakatsu K, "ABERRANT PANICLE ORGANIZATION 2", RFL, the rice ortholog of Arabidopsis LEAFY, suppresses the transition from inflorescence meristem to floral meristem through interaction with APO1, 2012, pp.69, 168~180
- Ikeda M, "Genes offering the potential for designing yield-related traits in rice", Curr Opin Plant Biol, 16, pp.213~220 (2013)
- Kim SR, "Development and validation of allele-specific SNP", indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sativa L, 2016, pp.9, 12~
- Lau WC, "Review of functional markers for improving cooking, eating, and the nutritional qualities of rice", Front Plant Sci, 6, pp.832~ (2015)
- Li M, "Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR", Cas9System, 2016, pp.7, 377~
- Lu L, "Evolution and association analysis of Ghd7 in rice", PLoS One, 7, pp.e34021~ (2012)
- Ookawa T, "New approach for rice improvement using a pleiotropic QTL gene for lodging resistance and yield", Nat Commun, 1, pp.132~ (2010), Springer Nature[DOI]
- Qi J, "Mutation of the rice Narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport", Plant Physiol, 147, pp.1947~1959 (2008)
- Rashid MA, "Nucleotide diversity, natural variation, and evolution of Flexible culm-1and Strong culm-2 lodging resistance genes in rice", Genome, 59, pp.473~483 (2016)
- Sun H, "Heterotrimeric G proteins regulate nitrogen-use efficiency in rice", Nat Genet, 46, pp.652~656 (2014)
- Takai T, "A natural variant of NAL1, selected in high-yield rice breeding programs, pleiotropically increases photosynthesis rate", Sci Rep, 3, pp.2149~ (2013)
- van Ittersum MK, "Can sub-Saharan Africa feed itself?", Proc Natl Acad Sci USA, 113, pp.14964~14969 (2016)
- Wang J, "Artificial Selection of Gn1a Plays an Important role in Improving Rice Yields Across Different Ecological Regions", Rice (NY), 8, pp.37~ (2015)
- Wang S, "Control of grain size, shape and quality by OsSPL16 in rice", Nat Genet, 44, pp.950~954 (2012)
- Wang S, "The OsSPL16-GW7regulatory module determines grain shape and simultaneously improves rice yield and grain quality", Nat Genet, 47, pp.949~954 (2015)
- Xu JL, "SS1(NAL1)- and SS2-Mediated Genetic Networks Underlying Source-Sink and Yield Traits in Rice (Oryza sativa L.)", PLoS One, 10, pp.e0132060~ (2015)
- Xue W, "Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice", Nat Genet, 40, pp.761~767 (2008)
- Zhang GH, "LSCHL4 from Japonica Cultivar, which is allelic to NAL1, increases yield of indica super rice 93-11", Mol Plant, 7, pp.1350~1364 (2014)
- Zhang J, "Combinations of the Ghd7, Ghd8 and Hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice", New Phytol, 208, pp.1056~1066 (2015)
- Zhao M, "Variations in DENSE AND ERECT PANICLE 1(DEP1) contribute to the diversity of the panicle trait in high-yielding japonica rice varieties in northern China", Breed Sci, 66, pp.599~605 (2016)