해당화 열매 성숙단계에 따른 생리활성 및 기능성 물질 변화 분석
Variation in bioactive principles and bioactive compounds of Rosa rugosa fruit during ripening
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초록
Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.
Abstract
Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.
참고문헌 (42)
- Bedon F, "Subgroup 4 R2R3-MYBs in conifer trees:gene family expansion and contribution to the isoprenoid-and flavonoid-oriented responses", J Exp Bot, 61, pp.3847~3864 (2010)
- Beltran G, "Influence of fruit ripening process on the natural antioxidant content of Hojiblanca virgin olive oils", Food Chem, 89, pp.207~215 (2005)
- Carrari F, "Metabolic regulation underlying tomato fruit development", J Exp Bot, 57, pp.1883~1897 (2006)
- Cartea ME, "Phenolic compounds in Brassica vegetables", Molecules, 16, pp.251~280 (2011)
- 최경순, "해당화 열매 추출 분말의 항산화 활성과 고지방식이로 유도된 mice의 혈청지질 수준에 미치는 영향", 한국식품영양학회지, 28(2), pp.320~327 (2015), 한국식품영양학회
- 최성희, "Essential Oil Components in Herb Teas (Rose and Rosehip)", 생명과학회지, 19(9), pp.1333~1336 (2009), 한국생명과학회
- Chiocchio I, "Screening of a hundred plant extracts as tyrosinase and elastase inhibitors, two enzymatic targets of cosmetic interest", Ind Crop Prod, 122, pp.498~505 (2018)
- Christenhusz MJ, "The number of known plants species in the world and its annual increase", Phytotaxa, 261, pp.201~217 (2016)
- Chrubasik C, "A systematic review on the Rosa canina effect and efficacy profiles", Phytother Res, 22, pp.725~733 (2008)
- Cordenunsi BR, "Influence of cultivar on quality parameters and chemical composition of strawberry fruits grown in Brazil", J Agric Food Chem, 50, pp.2581~2586 (2002)
- Dai J, "Plant phenolics: extraction, analysis and their antioxidant and anticancer properties", Molecules, 15, pp.7313~7352 (2010)
- Duan J, "Direct interaction between caffeic acid phenethyl ester and human neutrophil elastase inhibits the growth and migration of PANC-1 cells", Oncol Rep, 37, pp.3019~3025 (2017)
- Gulcin I, "Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid)", Toxicology, 217, pp.213~220 (2006)
- Gull J, "Variation in antioxidant attributes at three ripening stages of guava (Psidium guajava L.) fruit from different geographical regions of Pakistan", Molecules, 17, pp.3165~3180 (2012)
- 한유리, "플라보노이드 세포 수송 기전", 한국식품과학회지, 45(2), pp.137~141 (2013), 한국식품과학회[DOI]
- Harborne JB, "Advances in flavonoid research since 1992", Phytochemistry, 55, pp.481~504 (2000)
- Ha SH, "Carotenoids biosynthesis and their metabolic engineering in plants", J Plant Biotechnol, 30, pp.81~95 (2003)
- Hyun TK, "Variation in bioactive principles of Korean black raspberry (Rubus coreanus Miquel)during ripening", Plant Omics, 7, pp.510~ (2014)
- Jung HJ, "19α-hydroxyursane-type triterpenoids: antinociceptive anti-inflammatory principles of the roots of Rosa rugosa", Biol Pharm Bull, 28, pp.101~104 (2005)
- 강남성, "해당화의 과육 및 종자추출물의 대식세포 면역조절작용", 한국자원식물학회지, 23(5), pp.399~405 (2010), 한국자원식물학회
- 김미연, "퍼머넌트 웨이브와 산성 염색 반복시술시 로즈힙 추출물 첨가의 효능", 패션 비즈니스, 17(2), pp.151~163 (2013), 한국패션비즈니스학회[DOI]
- Lee J, "Development of a method predicting the oxidative stability of edible oils using 2, 2-diphenyl-1-picrylhydrazyl (DPPH)", Food chem, 103, pp.662~669 (2007)
- Morris GM, "AutoDock4 and AutoDockTools4:Automated docking with selective receptor flexibility", J Comput Chem, 30, pp.2785~2791 (2009)
- Radhakrishnan Narayanaswamy AI, "Molecular docking analysis of selected Clinacanthus nutans constituents as xanthine oxidase, nitric oxide synthase, human neutrophil elastase, matrix metalloproteinase 2, matrix metalloproteinase 9 and squalene synthase inhibitors", Pharmacog mag, 12, pp.S21~ (2016)
- Narayanaswamy R, "Molecular docking analysis of natural compounds as Human neutrophil elastase (HNE) inhibitors", J Chem Pharm Res, 5, pp.337~341 (2013)
- Narayanaswamy R, "In silico analysis of selected honey constituents as human neutrophil elastase (HNE) and matrix metalloproteinases (MMP 2 and 9)inhibitors", Int J Food Prop, 18, pp.2155~2164 (2015)
- 오현정, "제주산 참다래의 품종 및 숙도에 따른 화학성분과 항산화 활성", 한국식품영양과학회지, 40(3), pp.343~349 (2011), 한국식품영양과학회
- Okawa M, "Study on the antioxidative and antiproliferative activity of the polyphenol contained some functional foods", PhD, pp.2005
- 박병재, "해당화 줄기 및 뿌리의 주성분 분리 및 항산화활성", 한국자원식물학회지, 21(5), pp.402~407 (2008), 한국자원식물학회
- 박영기, "대추(Zyziphus jujuba) 품종별 성숙정도에 따른 항산화 활성, 총페놀 함량, 비타민 C 함량 및 당함량", 한국자원식물학회지, 29(5), pp.539~546 (2016), 한국자원식물학회[DOI]
- Pandey KB, "Plant polyphenols as dietary antioxidants in human health and disease", Oxid Med Cell Longev, 2, pp.270~278 (2009)
- Parsons TR, "Discussion of spectrophotometric determination of marine plant pigments, with revised equation for ascertaining chlorophylls and carotenoids", J mar Res, 21, pp.155~163 (1963)
- Prior RL, "Oxygen radical absorbance capacity (ORAC):New horizons in relating dietary antioxidants", bioactives and health benefits, 2015, pp.18, 797~810
- Surveswaran S, "Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants", Food chem, 102, pp.938~953 (2007)
- Seo EJ, "Interaction of antihistaminic drugs with human translationally controlled tumor protein (TCTP) as novel approach for differentiation therapy", Oncotarget, 7, pp.16818~ (2016)
- Shin Y, "Harvest maturity, storage temperature and relative humidity affect fruit quality, antioxidant contents and activity, and inhibition of cell proliferation of strawberry fruit", Postharvest Biol Tec, 49, pp.201~209 (2008)
- Tsuji N, "The Role of Elastases Secreted by Fibroblasts in Wrinkle Formation:Implication Through Selective Inhibition of Elastase Activity", Photochem Photobiol, 74, pp.283~290 (2001)
- Wahyu Widowati, "Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds", Natural Product Sciences, 23(3), pp.192~200 (2017), 한국생약학회
- 양원태, "육종용 추출물의 미백과 항노화 효과", Journal of Plant Biotechnology, 43(4), pp.492~499 (2016), 한국식물생명공학회
- Zhang J, "Metabolic profiling of strawberry (Fragaria×ananassa Duch.)during fruit development and maturation", J Exp Bot, 62, pp.1103~1118 (2010)
- Zifkin M, "Gene expression and metabolite profiling of developing highbush blueberry fruit indicates transcriptional regulation of flavonoid metabolism and activation of abscisic acid metabolism", Plant Physiol, 158, pp.200~224 (2012)
- Zvi MMB, "PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers", New Phytol, 195, pp.335~345 (2012)