부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산
In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins
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초록
액체배양을 통한 부채마의 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 steroidal saponin 생산과의 관계를 조사하였다. NAA와BA 농도별 부정근 생장은 NAA를 단독으로 1.0 mg/L를 처리한 곳에 서 가장 효과적이었으나 NAA에 BA를 첨가하면 부정근의 생장에 오히려 억제효과를 보였다. 20종의 각각 다른 배양주로부터 dioscin, prosapogenin A 및 prosapogenin C의 함 량을 조사한 결과 배양주에 따라큰 차이를 보였는데, 그 중 dioscin 함량이 건물당 2.5%로 가장 높았던 10번 배양주를 선발하였다. 부정근에 함유된 세 종류의 steroidal saponin 함량은 부정근의 생장이 왕성한 4주까지는 낮으나 부정근의 생장이 거의 정지되는 5주째에 급격히 증가하였다. Dioscin과 prosapogenin C의 함량은 NAA보다 IBA를 처리한 곳에서 현저히 높으나 prosapogenin A의 함량은 이들 두성 분의 종류와 농도에 영향을 받지 않았다.
Abstract
Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins,such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass product ogenin A and prosapogenin C.
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