활성산소종이 무궁화 꽃의 노화에 미치는 영향
Effect of reactive oxygen species on floral senescence in Hibiscus syriacus L.
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활성산소종이 무궁화 꽃의 노화에 미치는 영향을 알아보기 위 해, 무궁화 꽃의 개화직전 단계에서부터 노화에 이르기까지 가시 적인 꽃잎의 형태 변화를 경시적으로 관찰함과 동시에, 그에 따르 는 꽃잎의 수분 함량이나 조직의 산화 지표가 되는 MDA, 항산화 계 효소 활성의 변화 및 H2O2 함량 등에 대해서 조사하였다. 그 결 과 무궁화 꽃은 개화 후 24시간이 지나면서부터 꽃잎의 in-rolling이 시작되며 36시간 경과하면 50% 이상의 in-rolling이 관찰되었다. 꽃 의 생체중은 개화직후에 비교하여 약 30% 정도 감소하여 노화에 수반해 수분 손실이 관찰되었다. 개화 후 36시간에 이르기까지, 지 질과산화의 지표인 MDA나 항산화계 효소인 CAT, APX, GR의 활 성은 크게 변화가 없었다. 그러나 꽃잎이 완전하게 탈수증상을 나 타내는 노화 후기 (48h)에 항산화 효소의 활성 증가가 관찰되었으 며, H2O2 함량이 크게 증가하는 것으로 나타났다. 이러한 결과로부 터, 무궁화 꽃의 노화에 있어서 활성산소종은 초기 노화의 유도보 다는 꽃잎에 탈수증상이 나타나는 노화 후기에 관여하여 노화를 가속화하는 것으로 판단된다.
Abstract
To understand the effect of reactive oxygen species (ROS) on floral senescence in Hibiscus syriacus L., we have investigated change in relative water potential, malondialdehyde (MDA) content, H2O2 content and the activity of antioxidative enzymes in the petals during flower opening and senescence. Hibiscus flowers were achieved full bloom at early morning and started to in-rolling and showed petal in-rolling over than 50% at 24 h and 36 h after full bloom, respectively. The flower was a decrease in fresh weight by 30% and showed water loss with floral senescence. MDA content and activity of antioxidative enzymes such as APX, GR and CAT were showed no significant change until 36 h after full bloom. In the flower 48 h after full bloom that showed complete petal in-rolling and wilting, however, activity of antioxidative enzymes and H2O2 content was greatly increased as compared with 0 h after full bloom. These results suggest that reactive oxygen species are related to accelerating the later senescence more than inducing the early senescence during Hibiscus flower senescence.
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