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환경 스트레스에 관여하는 애기장대 BLH 8, BEL1-Like Homeodomain 8의 기능 분석

Functional characterization of Arabidopsis thaliana BLH 8, BEL1-Like Homeodomain 8 involved in environmental stresses

박지영|윤대진|백동원|박형철
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2011.06|Journal of Plant Biotechnology|제38권 제2호|pp. 162-168

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키워드

Signal transductionSalt stressHomeodomainBEL1-likeEnvironmental stress response
주제분류농학

초록

고염 스트레스는 식물의 성장과 수확량에 치명적인 영향을 야기한다. 그와 같은, 환경 스트레스에 의하여 식물은다양한 유전자의 발현으로 저항성을 가지게 하는 기작이발달되어 있다. 본 연구에서는 애기장대에서 다양한 환경 스트레스에 관여하는 유전자를 분리할 목적으로 GGM (Graphical Gaussian Model) program을 사용한 후, BLH8(BEL1-Like Homeodomain Gene 8) 유전자의 돌연변이 식물체를 구축하였다. atblh8-1 돌연변이체는 고농도의 Na+과 K+ 이온에 특이적으로 백화현상을 보이지만, 뿌리 성장에는 변화를 보이지 않았다. 그러므로, BLH8 단백질은Na+과 K+과 같은 환경스트레스 저항성에 관여하는 중요한 인자임을 시사한다. 이와 같이, GGM program은 환경스트레스에 관여하는 유전자를 분리하기 위한 유용한 도구일 것으로 사려된다.

Abstract

High salinity is a common stress condition that adversely affects plant growth and crop production. In response to various environmental stresses, plants activate a number of defense genes that function to increase the tolerance. To isolate Arabidopsis genes that are involved in abiotic stress responses, we carried out genetic screening using various mutant lines. Among them, the blh8 (BEL1- Like Homeodomain 8) mutant specifically shows chlorotic phenotypes to ionic (specifically, Na+ and K+) stresses, but no differences in root growth. In addition, BLH8 is related to plant development and abiotic stress as predicted by a Graphical Gaussian Model (GGM) network program. It implies that BLH8 functions as a putative transcription factor related to abiotic stress responses. Collectively, our results show that gene network analysis is a useful tool for isolating genes involved in stress adaptation in plants.

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