본문 바로가기
KERD
논문KCI 등재

Overexpression of starch branching enzyme 1 gene improves eating quality in japonica rice

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류농학

초록

Eating quality of rice attracts more and more attention from rice-eating consumers in the recent years. Thus, improvement of eating quality of cooked rice has become one of the most important breeding goals in japonica rice. Here, the generation of transgenic japonica rice with improved eating quality and grain yield are reported. Overexpression of OsSbe1 gene encoding rice starch branching enzyme 1 was driven by 35S promoter. Eleven independent homozygous T3 transgenic lines were characterized and had shown higher palatability (71.2 ~ 72.6) than wild type Gopum (70.4). Moreover, transgenic rice lines showed an increase in 1000-grain weight and number of spikelets per panicle compared with the wild type. The yield of milled rice was 562.8 ~ 596.7 kg/10a in eight T3 lines, but 542.1 kg/10a in wild type. Gene expression analyses in mRNA transcription and enzyme activity levels suggest that improved eating quality is due to the up-regulation of OsSbe1 gene.

Abstract

Eating quality of rice attracts more and more attention from rice-eating consumers in the recent years. Thus, improvement of eating quality of cooked rice has become one of the most important breeding goals in japonica rice. Here, the generation of transgenic japonica rice with improved eating quality and grain yield are reported. Overexpression of OsSbe1 gene encoding rice starch branching enzyme 1 was driven by 35S promoter. Eleven independent homozygous T3 transgenic lines were characterized and had shown higher palatability (71.2 ~ 72.6) than wild type Gopum (70.4). Moreover, transgenic rice lines showed an increase in 1000-grain weight and number of spikelets per panicle compared with the wild type. The yield of milled rice was 562.8 ~ 596.7 kg/10a in eight T3 lines, but 542.1 kg/10a in wild type. Gene expression analyses in mRNA transcription and enzyme activity levels suggest that improved eating quality is due to the up-regulation of OsSbe1 gene.

참고문헌 (66)

  1. Ahmed FE, "Detection of genetically modified organism in foods", Trends in Biotechnology, 20, pp.215~223 (2002)
  2. Aluru M, "Generation of transgenic maize with enhanced provitamin A content", Journal of Experimental Botany, 59(13), pp.3551~3562 (2008)
  3. Bao JS, "Microsatellites, single nucleotide polymorphisms and a sequence tagged site in relation to starch physicochemical properties in nonwaxy rice (Oryza sativa L.)", Theoretical and Applied Genetics, 113, pp.1185~1196 (2006)
  4. Bao JS, "Genetic control of the paste viscosity characteristics in indica rice (Oryza sativa L.)", Theoretical and Applied Genetics, 98, pp.1120~1124 (1999)
  5. Baxter G, "Effects of prolamin on the textural and pasting properties of rice flour and starch", Journal of Cereal Science, 40, pp.205~211 (2004)
  6. Chen XH, "Introduction of antisense Sbe genes into rice to increase its amylose content", PhD, pp.2003
  7. Yong-Gu Cho, "Identification of quantitative trait loci for physical and chemical properties of rice grain", Plant Biotechnology Reports, 4(1), pp.61~73 (2010), 한국식물생명공학회
  8. Chu CC, "Establishment of anefficient medium for anther culture of rice through comparative experiments on thenitrogen sources", Sci Sinica, 18, pp.659~668 (1975)
  9. Cunniff P, "Official methods of analysis of AOAC international. Arlington", pp.30~ (1995), AOAC international
  10. Denyer K, "The control of amylose synthesis", Journal of Plant Physiology, 158, pp.479~487 (2001)
  11. Dian WM, "Evolution and expression analysis of starch synthase III and IV in rice", Journal of Experimental Botany, 56(412), pp.623~632 (2005)
  12. Ding JY, "Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes", Journal of Agricultural and Food Chemistry, 52, pp.3372~3377 (2004)
  13. Ebitani T, "Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japonica elite cultivar ‘Koshihikari’", Breeding Science, 55, pp.65~73 (2005)
  14. Fujita N, "Antisense inhibition of isoamylase alters the structure of amylopectin and the physicochemical properties of starch in rice endosperm", Plant Cell Physiology, 44(6), pp.607~618 (2003)
  15. Fujita N, "Characterization of pullulanase (PUL)-deficient mutants of rice (Oryza sativa L.) and the function of PUL on starch biosynthesis in the developing rice endosperm", Journal of Experimental Botany, 60(3), pp.1009~1023 (2009)
  16. Fujita N, "Function and characterization of starch synthase 1 using mutants in rice", Plant Physiology, 140, pp.1070~1084 (2006)
  17. Fujita N, "Characterization of SSIIIa-deficient mutants of rice: the function of SSⅢa and pleiotropic effects by SSIIIa deficiency in the rice endosperm", Plant Physiology, 144, pp.2009~2023 (2007)
  18. Hanashiro I, "Granule-bound starch synthase 1 is responsible for biosynthesis of extra-long unit chains of amylopectin in rice", Plant Cell Physiology, 49(6), pp.925~933 (2008)
  19. Hawker JS, "Interaction of spinach leaf adenosinediphosphate glucose α-1, 4-glucan α-4-glucosyl transferase and α-1,4-glucan, α-1, 4-glucan-6-glucosyl transferase in synthesis of branched α -glucan", Archives of Biochemistry and Biophysics, 160, pp.530~551 (1974)
  20. Hong HC, "Studies on the degree of polymerization of amylopectin and eating quality characteristics in rice (Oryza sativa L.)", PhD, pp.2006
  21. Huang YS, "Present situation and prospects for the research on rice grain quality forming", Chinese Journal of Rice Science, 12(3), pp.172~176 (1998)
  22. Hwang HG, "QTL analysis for eating quality in japonica rice", pp.60~63 (2005), Rice is life: scientific perspectives for the 21 century
  23. Jiang LX, "Jiang LX, Yang LT, Zhang HB, Guo JC, Mazzara M, Eede GV, Zhang DB (2009) International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified rice", Journal of Agricultural and FoodChemistry, 57, pp.3525~3532 (2009)
  24. 구홍광, "일품벼", 모로베레칸 이입계통을 이용한 미질특성 관련 QTL 분석, 2009, pp.41(4), 429~436[DOI]
  25. Juliano BO, "Varietal impact on rice quality", Cereal Food World, 43(4), pp.207~211 (1998)
  26. Keeling PL, "Biochemistry and genetics of starch synthesis", Annual Review of Food Science and Technology, 1, pp.271~303 (2010)
  27. Kim WS, "Expression of Escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching", Planta, 220, pp.689~695 (2005)
  28. Kim YH, "Genetic transformation protocol of rice. Plant genetic transformation.", pp.173~179 (2007), Jungmunkag publisher
  29. Kubo A, "Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase 1 gene supports a direct role for isoamylase 1 in amylopectin biosynthesis", Plant Physiology, 137, pp.43~56 (2005)
  30. Laura B, "Review of GMO detection and quantification techniques" (2002), Europena Commission, EUR
  31. Lee JS, "QTL analysis for grain quality properties in a japonica rice combination", PhD, pp.2003
  32. Lestari P, "PCR marker-based evaluation of the eating quality of japonica rice (Oryza sativa L.)", Journal of Agricultural and Food Chemistry, 57, pp.2754~2762 (2009)
  33. Little RR, "Differential effect of dilute alkali on 25 varieties of milled white rice", Cereal Chemistry, 35, pp.111~126 (1958)
  34. Liu Q, "Food Carbohydrates: Chemistry, Physical Properties, and Application", pp.309~355 (2005), Taylor and Francis Group, Boca Raton
  35. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method", Methods, 25, pp.402~408 (2001)
  36. Mei SF, "A simplified method for the determination of amylose content in rice", Journal of Nuclear Agricultural Sciences, 21(3), pp.246~248 (2007)
  37. Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
  38. Nakamura Y, "Towards a better understanding of the metabolic system for amylopectin biosynthesis in plants: rice endosperm as a model tissue", Plant Cell Physiology, 43(7), pp.718~725 (2002)
  39. Nakamura Y, "Essential amino acids of starch synthase IIa differentiate amylopectin structure and starch quality between japonica and indica rice varieties", Plant Molecular Biology, 58, pp.213~227 (2005)
  40. Nakamura Y, "Carbohydrate metabolism in the developing endosperm of rice grains", Plant and Cell Physiology, 30(6), pp.833~839 (1989)
  41. Nielsen TH, "Intermediary glucan structures formed during starch granule biosynthesis are enriched in short side chains, a dynamic pulse labeling approach", The Journal of Biological Chemistry, 277, pp.20249~20255 (2002)
  42. Nishi A, "Biochemical and genetic analysis of the effects of amylose-extender mutation in rice endosperm", Plant Physiology, 127, pp.459~472 (2001)
  43. Ohdan T, "Expression profiling of genes involved in starch synthesis in sink and source organs of rice", Journal of Experimental Botany, 56(422), pp.3229~3244 (2005)
  44. Ong MH, "Texture determinants in cooked, parboiled rice. 1: Rice starch amylose and the fine structure of amylopectin", Journal of Cereal Science, 21, pp.251~260 (1995)
  45. Ong MH, "Texture determinants of cooked, parboiled rice. 2: Physicochemical properties and leaching behavior of rice", Journal of Cereal Science, 21, pp.261~269 (1995)
  46. Peng S, "Relationship between variation in activies of key enzymes related to starch synthesis during grain filling period and quality of eating and cooking in rice", Rice Science, 13(1), pp.43~50 (2006)
  47. Perez CM, "Modification of the simplified amylose test for milled rice", Starch, 1978, pp.30, 424~426
  48. Reddy KR, "The fine structure of rice starch amylopectin and its relation to the texture of cooked rice", Carbohydrate Polymers, 22, pp.267~275 (1993)
  49. Satoh H, "Nakamura Y(2003)Starch-branching enzyme 1-deficient mutation specifically affects the structure and properties of starch in rice endosperm", Plant Physiology, 133, pp.1111~1121 (2003)
  50. Shen P, "Relationship between variation in activities of key enzymes related to starch synthesis during grain filling period and quality of eating and cooking in rice", Rice Science, 13(1), pp.43~50 (2006)
  51. Shu QY, "Relationship between RVA profile character and eating quality in Oryza sativa L", Scientia Agricultura Sinica, 31(3), pp.25~29 (1998)
  52. Stawski D, "New determination method of amylose content in potato starch", Food Chemistry, 110, pp.777~781 (2008)
  53. Sun MM, "Molecular aspect of good eating quality formation in japonica rice", Plos One, 6(4), pp.e18385~ (2011)
  54. Takeuchi Y, "QTL detection for eating quality including glossiness, stickness, taste andhardness of cooked rice", Breeding Science, 57, pp.231~242 (2007)
  55. Tanaka N, "The structure of starch can be manipulated by changing the expression levels of starch branching enzymeⅡb in rice endosperm", Plant Biotechnology Journal, 2, pp.507~516 (2004)
  56. Tanaka R, "Cultivation method and eating quality of paddy rice 1. Evaluation with mechanical taster of boiled rice so called “MIDO meter”", Tohoku Journal of Crop Science, 35, pp.45~46 (1992)
  57. Terada R, "Antisense waxy genes with highly active promoters effectively suppress waxy gene expression in transgenic rice", Plant Cell Physiology, 41(7), pp.881~888 (2000)
  58. Tian ZX, "Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities", Proceedings of the National Academy of Sciences of the United States of America, 106(51), pp.21760~21765 (2009)
  59. Utsumi Y, "Structural and enzymatic characterization of the isoamylase 1 homo-oligomer and the isoamylase1- isoamylase2 hetero-oligomer from rice endosperm", Planta, 225, pp.75~87 (2006)
  60. Wada T, "Mapping of QTLs for eating quality and physicochemical properties of the japonica rice ‘Koshihikari’", Breeding Science, 58, pp.427~435 (2008)
  61. Wong KS, "Structures and properties of amylopectin and phytoglycogen in the endosperm of sugary-1 mutants of rice", Journal of Cereal Science, 37, pp.139~149 (2003)
  62. Wu FX, "Content of amylose in rice (Indica sativa L.) changed by sense or antisense expression starch branching enzyme gene rbe1", Fujian Journal of Agricultural Sciences, 21(2), pp.150~153 (2006)
  63. Yamanouchi H, "Organ specificity of isoforms of starch branching enzyme (Q-Enzyme)", Plant Cell Physiology, 33(7), pp.985~991 (1992)
  64. Yang LT, "Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR", Plant Cell Reports, 23, pp.759~763 (2005)
  65. Zeeman SC, "Starch: its metabolism, evolution, and biotechnological modification in plants", Annual Review of Plant Biology, 61, pp.209~234 (2010)
  66. Zhao BH, "Changes in activities of the key enzymes related to starch synthesis in rice grains during grain filling and their relationships with the filling rate and cooking quality", Agricultural Sciences in China, 4(1), pp.26~33 (2005)

관련 논문AI추천

이 간행물의 다른 논문 (5건)