Transcriptional profiles of Rhizobium vitis-inoculated and salicylic acid-treated ‘Tamnara’ grapevines based on microarray analysis
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The transcriptional profiles of ‘Tamnara’ grapevine (Vitis labruscana L.) to Rhizobium vitis were determined using 12,000 gene oligonucleotide microarray chips constructed with 6,776 unigenes based on the EST sequencing. Among them, 95 clones were up-regulated more than three times and 90 were down-regulated more than 5-times in the R. vitis-inoculated grapevines relative to the control vines. Treatment of salicylic acid showed that 337 clones were upregulated and 52 clones were down regulated in grapevines. Microarray analysis, reverse transcription-polymer chain reaction, and slot blot hybridization analysis revealed that 5, 14, and 64 clones were up-regulated and 10, 12, and 61 clones were down-regulated in wounded, salicylic acid- treated, and R. vitis-inoculated ‘Tamnara’ grapevine leaves, respectively. The expression patterns of β-1,3-glucanase, proline-rich protein, and lipoxygenase genes of ‘Tamnara’ moderately resistant to R. vitis were similar to those of resistant ‘Concord’ and ‘Delaware’ grapevines. However, chalcone synthase genes in ‘Tamnara’ grapevines showed similar expression patterns to susceptible grapevines ‘Neomuscat’ and ‘Rizamat’. Further expression studies with various clones for each gene should be conducted to elucidate their roles in resistant responses against pathogens or other stimuli in grapevines. These results could provide better resources for understanding the mechanism of defense responses against crown gall disease and clues for identifying new genes that may play a role in defense against R. vitis in grapevines.
Abstract
The transcriptional profiles of ‘Tamnara’ grapevine (Vitis labruscana L.) to Rhizobium vitis were determined using 12,000 gene oligonucleotide microarray chips constructed with 6,776 unigenes based on the EST sequencing. Among them, 95 clones were up-regulated more than three times and 90 were down-regulated more than 5-times in the R. vitis-inoculated grapevines relative to the control vines. Treatment of salicylic acid showed that 337 clones were upregulated and 52 clones were down regulated in grapevines. Microarray analysis, reverse transcription-polymer chain reaction, and slot blot hybridization analysis revealed that 5, 14, and 64 clones were up-regulated and 10, 12, and 61 clones were down-regulated in wounded, salicylic acid- treated, and R. vitis-inoculated ‘Tamnara’ grapevine leaves, respectively. The expression patterns of β-1,3-glucanase, proline-rich protein, and lipoxygenase genes of ‘Tamnara’ moderately resistant to R. vitis were similar to those of resistant ‘Concord’ and ‘Delaware’ grapevines. However, chalcone synthase genes in ‘Tamnara’ grapevines showed similar expression patterns to susceptible grapevines ‘Neomuscat’ and ‘Rizamat’. Further expression studies with various clones for each gene should be conducted to elucidate their roles in resistant responses against pathogens or other stimuli in grapevines. These results could provide better resources for understanding the mechanism of defense responses against crown gall disease and clues for identifying new genes that may play a role in defense against R. vitis in grapevines.
참고문헌 (37)
- Anand A, "Salicylic acid and systemic acquired resistantce play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens", Plant Physiol, 146, pp.703~715 (2008)
- Benjamini Y, "Controlling the false discovery rate, a practical and powerful approach to multiple testing", J Royal Stat Soc Ser B, 57, pp.289~300 (1995)
- Burr TJ, "Crown gall of grape: Biology and disease management", Annu Rev Phytopathol, 37, pp.53~80 (1999)
- Chang S, "A simple and efficient method for isolating RNA from pine trees", Plant Mol Biol, 11, pp.113~116 (1993)
- Cheong YH, "Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis", Plant Physiol, 129, pp.661~677 (2002)
- Choi, YJ, "Screening genes expressed by Rhizobium vitis inoculation and salicylic acid treatment in grapevines using GeneFishing", J Jpn Soc Hort Sci, 77, pp.137~142 (2008)
- Choi, YJ, "Transcriptional profiling of ESTs responsive to Rhizobium vitis from ‘Tamnara’ grapevines (Vitis sp.)", J Plant Physiol, 167, pp.1084~1092 (2010)
- Cramer GR, "Effects of abiotic stress on plants: a systems biology perspective", BMC Plant Biol, 11, pp.163~ (2011)
- Creelman RA, "Biosynthesis and action of jasmonates in plants", Annu Rev Plant Physiol Plant Mol Biol, 48, pp.355~381 (1997)
- Deluc LG, "Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development", BMC Genomics, 8, pp.429~ (2007)
- Dong X, "SA, JA, ethylene, and disease resistance in plants", Curr Opin Plant Biol, 1, pp.316~323 (1998)
- Espinoza C, "Gene expression associated with compatible viral diseases in grapevine cultivars", Funct Integr Genomics, 7, pp.95~110 (2007)
- Figueiredo A, "Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi", J Exp Bot, 59, pp.3371~3381 (2008)
- 허윤영, "포도 유전체 연구현황 및 전망", Journal of Plant Biotechnology, 42(4), pp.298~311 (2015), 한국식물생명공학회[DOI]
- Kandler O, "Storage carbohydrates in vascular plants", pp.115~131 (1984), Cambridge Univ. Press
- Keller F, "Photoassimilate distribution in plants and crops: Source-sink relationships", pp.157~183 (1996), Marcel Dekker
- Kerr A, "Biological control of crown gall through production of agrocin 84", Plant Dis, 64, pp.28~30 (1980)
- Kunkel BN, "Crosstalk between signaling pathways in pathogen defense", Curr Opin Plant Biol, 5, pp.325~331 (2002)
- Lin H, "Comparative analysis of ESTs involved in grape responses to Xylella fastidiosa infection", BMC Plant Biol, 7, pp.8~ (2007)
- Malamy J, "Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection", Science, 250, pp.1002~1004 (1990)
- Maleck K, "Defense on multiple fronts: How do plants cope with diverse enemies?", Trends Plant Sci, 4, pp.215~219 (1999)
- Meyers BC, "Methods for transcriptional profiling in plants. Be fruitful and replicate", Plant Physiol, 135, pp.637~652 (2004)
- Nishizawa A, "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage", Plant Physiol, 147, pp.1251~1263 (2008)
- Park KH, "Incidence of severe crown gall disease on tetraploid cultivars of grape in Korea", Plant Pathol J, 16, pp.290~293 (2000)
- 박교선, "내병성 고품질 조생종 포도 품종 '탐나라' 육성", 원예과학기술지, 22(4), pp.458~461 (2004), 한국원예학회
- Peterbauer T, "Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds", Seed Sci Res, 11, pp.185~197 (2001)
- Peterbauer T, "Functional expression of a cDNA encoding pea (Pisum sativum L.)raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis", Planta, 215, pp.839~849 (2000)
- Jeong Ho Roh, "Histochemical Changes by Agrobacterium vitis Infection in Grapevines", Horticulture, Environment, and Biotechnology, 46(4), pp.260~263 (2005), 한국원예학회
- Shah J, "The salicylic acid loop in plant defense", Curr Opin Plant Biol, 6, pp.365~371 (2003)
- Schulze A, "Navigating gene expression using microarrays: A technology review", Nature, 3, pp.E190~E195 (2001)
- Stears RL, "Trends in microarray analysis", Nature, 9, pp.140~145 (2003)
- Stover EW, "Crown gall formation in a diverse collection of Vitis genotypes inoculated with Agrobacterium vitis", Am J Enol Vitic, 48, pp.26~32 (1997)
- Terrier N, "Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development", Planta, 222, pp.832~847 (2005)
- The Franch-Italian Public Consortium for Grapevine, "The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla", Nature, 449, pp.463~467 (2007)
- Van Gelder RN, "Amplified RNA synthesized from limited quantities of heterogeneous cDNA", Proc Natl Acad Sci USA, 87, pp.1663~1667 (1990)
- Waters DL, "cDNA microarray analysis of developing grape (Vitis vinifera cv. Shiraz) berry skin", Funct Integr Genomics, 5, pp.40~58 (2005)
- 윤해근, "병원균 접종에 의한 포도 줄기흑병 저항성 검정 체계 개발", 원예과학기술지, 21(4), pp.325~328 (2003), 한국원예학회