Dehydroascorbate reductase 과발현 형질전환 감자 식물체의 단백질체 분석
Proteomic analysis of dehydroascorbate reductase transgenic potato plants
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아스코르빈산(ascorbic acid, AsA)는 강력한 항산화 물질및 환원제로서 식물에서 산화형 AsA인 dehydroascorbate (DHA)를 활성형인 환원형 AsA로 변환시키는 효소인dehydroascorbate reductase (DHAR)에 의해 생성된다. 선행연구의 결과로서, 본 연구팀은 참깨의 모상근에서 분리한 DHAR 유전자를 이용하여 항시 발현하는 CaMV 35S 프로모터와 괴경 특이적으로 발현하는 patatin 프로모터의 조절하에 과발현 시킨 형질전환 감자를 개발하였다형질전환 감자 식물체들은 비형질전환체 보다 증가된DHAR 활성과 AsA 함량을 보인 바 있다. 본 연구에서는단백질체 분석을 통해서 형질전환 감자 식물체에서DHAR 과발현에 의해 조절되는 단백질들을 조사하였다. 단백질체 분석의 결과로서, 형질전환 감자에서 도입된DHAR 단백질과 다양한 항산화 관련 단백질들이 식물 생장시기 동안 증가 하였다. 본 연구의 결과로서, 도입된유전자인 DHAR이 항산화 단백질들의 발현 증가를 통해형질전환 식물체의 스트레스 내성 기작을 향상 시킬 수있을 것으로 생각된다.
Abstract
Ascorbic acid (AsA) is a strong antioxidant/ reducing agent that can be converted to dehydroascorbate (DHA) by oxidation in plants. DHA, a very short-lived chemical, is recycled to AsA by dehydroascorbate reductase (DHAR). Previously, DHAR cDNA was isolated from the hairy roots of the sesame plant, and DHAR-overexpressing transgenic potato plants were generated under the control of the CaMV35S promoter (CaMV35S::DHAR). An increase in transgene expression and ascorbate levels were observed in the transgenic plants. In the present study, proteomic analysis revealed that transgenic plants not only accumulated DHAR in their cells, but also induced several other antioxidant enzyme-related proteins during plant growth. These results suggest that DHAR is important for stress tolerance via induction of antioxidant proteins, and could improve stress tolerance in transgenic potato plants.
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