수박계통간 염색체수준의 유전적변이 분석
Genome-wide analysis of sequence variations in eight inbred watermelon lines
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초록
수박의 형태적 변이의 유전적 원인을 분석해 보기 위해 8개계통에서 re-sequencing을 수행하였다. 유전적 변이의 수는염색체에 따라 다르게 나왔으며 발견된 SNP의 약 12.9%만이 유전자내에서 발견되었고 나머지는 프로모터나 유전자사이의 지역에서 발견되었다. SNP 밀도에 대한 분석 결과염색체 6번의 말단지역에 변이가 집중되어 있는 것을 알 수있었다. 또한 염색체 10과 11번에 잘 보존된 지역을 발견하였다. Pathway 분석을 통해 DIMBOA(일종의 항생제)-glucoside 분해 대사가 계통간 가장 차이나는 것으로 확인되었으며이는 각 계통의 병저항성에서 차이가날 가능성을 시사하는것이다. 당대사 관련 유전자 변이를 분석한 결과 alphagalactosidase 유전자에 가장 변이가 많은 것으로 밝혀졌다. 이러한 연구 결과는 육종을 분자수준에서 이해하는 데 도움을 줄 것으로 생각한다.
Abstract
To investigate the genetic basis of phenotypic differences, sequence variations were analyzed in 8 inbred watermelon lines by re-sequencing. The number of sequence variations differed depending on the chromosome. Only 12.9% of SNPs were found within genes, whereas the rest were detected in promoter or intergenic regions. SNP density analysis showed that there was a highly variable region at the end of chromosome 6, which is similar to previously published findings. However, this region with high SNP density did not show much variation between the lines. In contrast, highly conserved regions with a size of 6.5-10 Mb were found in chromosomes 10 and 11. Pathway analysis suggested that the DIMBOA (a natural antibiotic)-glucoside degradation pathway was significantly different between the lines, indicating that the eight lines may have different levels of pathogen resistance. Among the carbohydrate-related genes, the alpha-galactosidase gene was the most variable among the lines. Information from this study will be helpful in understanding the watermelon breeding process at the molecular level.
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