Xylella fastidiosa의 배양여액을 이용한 포도나무 피어스병 품종 저항성 검정
Evaluation of resistance to Pierce’s disease among grapevine cultivars by using the culture filtrates produced from Xylella fastidiosa
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초록
This study investigated whether culture filtrates produced by Xylella fastidiosa can be used to determine the varietal susceptibility to Pierce's disease in grapevines (Vitis spp.) as a substitute for pathogen inoculation or field screening. A bioassay of grape leaves with culture filtrates from the pathogen showed that their phytotoxicities were active and host-selective. Ethyl acetate extracts from them also showed toxicities and host selectivity in both bunches of grapes and muscadine grapes. The sensitive range of plants to the culture filtrates and their ethyl acetate extracts was consistent with the host range of the Pierce’s disease pathogen. Susceptible cultivars are sensitive to even highly diluted culture filtrates, while resistant cultivars were not affected even at their original culture filtrates. Susceptible cultivars were more sensitive to the undiluted culture filtrate than were highly diluted culture filtrates, and the younger leaves were the most sensitive to the culture filtrates in grapes. Although some European grape cultivars showed moderately susceptibility in this study, the determination of varietal resistance to Pierce’s disease by the treatment of culture filtrates of pathogens could provide valuable information for the preliminary selection of genetic resources and seedlings from hybridization in a disease resistant grape breeding program.
Abstract
This study investigated whether culture filtrates produced by Xylella fastidiosa can be used to determine the varietal susceptibility to Pierce's disease in grapevines (Vitis spp.) as a substitute for pathogen inoculation or field screening. A bioassay of grape leaves with culture filtrates from the pathogen showed that their phytotoxicities were active and host-selective. Ethyl acetate extracts from them also showed toxicities and host selectivity in both bunches of grapes and muscadine grapes. The sensitive range of plants to the culture filtrates and their ethyl acetate extracts was consistent with the host range of the Pierce’s disease pathogen. Susceptible cultivars are sensitive to even highly diluted culture filtrates, while resistant cultivars were not affected even at their original culture filtrates. Susceptible cultivars were more sensitive to the undiluted culture filtrate than were highly diluted culture filtrates, and the younger leaves were the most sensitive to the culture filtrates in grapes. Although some European grape cultivars showed moderately susceptibility in this study, the determination of varietal resistance to Pierce’s disease by the treatment of culture filtrates of pathogens could provide valuable information for the preliminary selection of genetic resources and seedlings from hybridization in a disease resistant grape breeding program.
참고문헌 (32)
- Agrios, GN, "Plant Pathology", pp.190~196 (2005), Elsivier Pte., Ltd
- Briggs, SP, "Genetic patterns of planthost-parasite interactions", Trend Genetics, 10, pp.12~16 (1994)
- Buiatti, M, "Phytotoxins as tools in breeding and selection of disease-resistant plants", Experientia, 47, pp.811~819 (1991)
- Durbin, RD, "The mechanism for self-protection against bacterial phytotoxins", Ann Rev Phytopathol, 26, pp.313~329 (1988)
- Friesen, TL, "The toxins produced by microorganisms in plant disease development", Cellular Microbiol, 10, pp.1421~1428 (2008)
- Gracen, VE, "Rapid method for screening resistance plants for control of southern corn leaf blight", Plant Dis Reptr, 55, pp.469~470 (1971)
- Grausgruber, H, "Chromosomal location of Fusarium head blight resistance and in vitro toxin tolerance in wheat using the Hobbit ‘sib’(Triticum macha) chromosome substitution lines", J Gen Breeding, 52, pp.173~180 (1998)
- Hopkins, DL, "Induced resistance to Pierce’s disease of grapevine by weakly virulent strains of Xylella fastidiosa", Proc. Int. Conf. Plant Pathol. Bacteria, 8th, pp.951~956 (1992)
- Hopkins, DL, "Tolerance to Pierce disease and the associated rickettsia-like bacterium in muscadine grape", J Amer Soc Hort Sci, 99, pp.436~449 (1974)
- Jayasankar, S, "In-vitro selection of Vitis vinifera Chardonay with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase", Planta, 211, pp.200~208 (2000)
- Johal, GS, "Reductase activity encoded by the HM1 disease resistance gene in maize", Science, 258, pp.985~987 (1992)
- Kitajima, H, "Diseases in fruit trees", pp.396~453 (1989), Youkendo Press
- Kohmoto, K, "Correlation between the susceptibility of apple cultivars to Alternaria mali and their sensitivity to AM-toxin I", Ann Phytopathol Soc Japan, 43, pp.65~68 (1977)
- Lemmens, M, "Breeding for head blight (Fusarium spp.) resistance in wheat:Development of a mycotoxin-based selection method of seedlings", ActaHorticulturae, 355, pp.223~232 (1994)
- Magarey, RD, "Anthracnose of grapevines", Plant Prot Quart, 8, pp.106~110 (1933)
- Markham, JE, "Host-selective toxins as agents of cell death in plant-fungus interactions", Mol Plant Pathol, 2, pp.229~239 (2001)
- Mayama, S, "Selection of somaclonal variants of oats resistant to Helminthosporium victoriae which produces a host specific toxin, victorin", Plant Tissue Culture Letters, 7, pp.64~68 (1990)
- Mortensen, J.A., "Breeding grapes for central Florida", Hort-Science, 6, pp.149~153 (1971)
- Mortensen, JA, "Sources of resistance to Pierce’s disease in Vitis", J Am Soc Hortic Sci, 102, pp.695~697 (1977)
- Otani, H, "Biological activities of AK-toxins I and II, Host-specific toxins from Alternaria alternata Japanese pear pathotype", Ann Phytopath Soc Japan, 51, pp.285~293 (1985)
- Park, JS, "evaluation of pear cultivar susceptibility to AK-toxin produced by Alternaria kikuchiana", Res Rep Agri Sci Tech CNU, Korea, 15, pp.1~8 (1988)
- Pearson, RC, "Compendium of grape diseases. Amer", pp.41~ (1988), Phytopathol Soc Press
- Pringle, PB, "Host-specific plant toxins", Ann Rev Phytopathol, 2, pp.133~156 (1964)
- Purcell, AH, "Vector preference and inoculation efficiency as components of resistance to Pierces disease in European grape cultivars", Phytopathology, 71, pp.429~435 (1981)
- Raju, BC, "Relative sensitivity of selected grapevine cultivars to Pierce’s disease bacterial inoculations", Amer J Enol Vitic, 32, pp.155~158 (1981)
- Saito, A, "Selection of mutants resistant to Alternaria blotch from in vitro-cultured apple shoots irradiated with X- and -rays", J Plant Physiol, 158, pp.391~400 (2001)
- Schertz, KF, "Inheritance of reaction of Sorghum bicolor to toxin produced by Periconia circinata", Crop Sci, 9, pp.621~624 (1969)
- Steiner, GW, "Partial characterization and use of a host-specific toxin from Helminthosporium sacchari on sugarcane", Phytopathology, 61, pp.691~695 (1971)
- Tanaka, A, "Insertional mutagenesis and cloning of the genes required for biosynthesis of the host-specific AK-Toxin in the Japanese pear pathotype of Alternaria alternata", Mol. Plant-Microbe Interac, 112, pp.691~702 (1999)
- Wheeler, H, "Mass screening for disease resistant mutant in oats", Science, 122, pp.1229~ (1963)
- 윤해근, "온실 내에서의 효율적인 포도 새눈무늬병 저항성 검정법 개발", Horticulture, Environment, and Biotechnology, 44(6), pp.809~812 (2003), 한국원예학회
- Yun, HK, "Use of host-specific toxins from Alternaria pathogens for determining disease susceptibility of crops. I. Evaluation of apple cultivar to Alternaria blotch", Korean J Plant Pathol, 8, pp.185~189 (1992)