Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)
Abstract
The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)
참고문헌 (26)
- Aish M, "Banana plantlet production through tissue culture", Pak J Bot, 36, pp.617~620 (2004)
- Ali A, "Initiation, Proliferation and Development of Micro-Propagation System for Mass Scale Production of Banana through Meristem Culture", African Journal of Biotechnology, 10, pp.15731~15738 (2011)
- "FAO Stat accessed vide"[URL]
- Heslop-Harrison J, "Domestication genomics and the future for banana", Ann Bot, 100, pp.1073~1084 (2007)
- Israeli Y Y, "In vitro culture of bananas", Fruits, 43, pp.219~223 (1995)
- Krikorian AA, "Aseptic culture techniques for banana and plantain improvement", Econom Bot, 38, pp.322~331 (1984)
- Ma SS, "In vitro formation of adventitious buds in banana shoot apex following decapitation", J Chinese Soci Hort Sci, 18, pp.135~142 (1972)
- Madhulatha P, "Influence of Liquid Pulse Treatment with Growth Regulators on in Vitro Propagation of Banana (Musa spp. AAA)", Plant Cell Tissue and Organ Culture, 76, pp.189~192 (2004)
- Madhulatha P, "Influence of liquid pulse treatment with growth regulators on in vitro propagation of banana (Musa spp. AAA)", Plant Cell, Tissue, Organ Cult, 76, pp.189~191 (2004)
- Martin KP, "RAPD analysis of a variant of banana (Musa sp.) cv. Grande naine and its propagation via shoot tip culture", In Vitro Cellular and Development Biology - Plant, 42, pp.188~192 (2006)
- Mendes BMJ, "Efficacy of banana plantlet production by micropropagation", Pesqui Agropecu Bras, 31, pp.863~867 (1996)
- Ngomuo M, "The in Vitro Propagation Techniques for Producing Banana Using Shoot Tip Cultures", American Journal of Plant Sciences, 5, pp.1614~1622 (2014)
- Ortiz R, "Crossbreeding to biotechnologyfacilitated improvement of banana and plantain", Biotec Advances, 32, pp.158~169 (2014)
- Pillay M, "Banana breeding: Progress and Challenges", pp.312~ (2011), CRC Publishers
- Ray T, "Genetic stability of three economically important micropropagated banana (Musa spp.) cultivars of lower IndoGangetic plains, as assessed by RAPD and ISSR markers", Plant Cell, Tiss Org, 85, pp.11~21 (2006)
- Robooni T, "Improved Propagation Techniques to Enhance the Productivity of Banana (Musa spp.)", Open Agriculture, 3, pp.138~145 (2018)
- Rout GR, "Biotechnology of the Banana : A Review of Recent Progress", Plant Biol, 2, pp.512~524 (2000)
- Roux N, "Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp", Plant Cell, Tiss Org Cult, 66, pp.189~197 (2001)
- Saraswathi MS, "Cost effective tissue culture media for large-scale propagation of three commercial banana (Musa spp.) varieties", The J Hort Sci Biotec, 91, pp.23~29 (2006)
- Saraswathi MS, "Cost effective tissue culture media for large-scale propagation of three commercial banana (Musa spp.) varieties", The J Hort Sci Biotec, 91, pp.23~29 (2016)
- Singh TD, "Analysis of growth, yield potential and horticultural performance of conventional vs. micropropagated plants of Curcuma longa var", Lakadong, Afr J Biotech, 12, pp.1604~1608 (2013)
- Stover NW, "Bananas", pp.123~ (1987), Longman
- Strosse H, "Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.)", Plant Sci, 170, pp.104~112 (2006)
- Venkatachalam L, "Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers", Electronic Journal of Biotechnology, 10(1) (2007)
- Thimmaraju L, "Direct shoot and cormlet regeneration from leaf explants of silk banana(AAB)", In Vitro Cell Dev Biol Plant, 42, pp.262~269 (2006)
- Vuylsteke DR, "Shoot-tip culture for the propagation, conservation and distribution of Musa germplasm", pp.82~ (1998), IITA