Induction of in vitro root tubers in Holostemma annulare (Roxb.) K. Schum. for the production of bioactive metabolites
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Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α -naphthaleneacetic acid. In addition, preliminary phytochemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.
Abstract
Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α -naphthaleneacetic acid. In addition, preliminary phytochemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.
참고문헌 (37)
- Balakrishnan A, "In vitro propagation of Holostemma ada-kodien Schult- an endangered medicinal plant", International Journal of Current Research in Life sciences, 6, pp.204~2206 (2018)
- Beena MR, "Rapid in vitro propagation of medicinally important Ceropegia candelabrum", Plant cell Tiss Org Cult, 72, pp.285~289 (2003)
- Decruse SW, "Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare", CryoLetters, 23, pp.55~60 (2002)
- Decruse SW, "Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips", Plant cell Tiss Org Cult, 76, pp.179~182 (2004)
- Decruse SW, "Cryopreservation of alginate coated shoot tips of in vitro grown Holostemma annulare (Roxb.) K. Schum, an endangered medicinal plant: influence of preculture and DMSO treatment on survival and regeneration", CryoLetters, 20, pp.243~250 (1999)
- Gayathri G, "Rapid multiplication of Fire flamed bush (Woodfordia fruticosa (L.) Kurz. through in vitro techniques", Current Biotica, 2, pp.453~460 (2009)
- Geetha SP, "Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants; Methods in Molecular biology (Vol. 547, Chapter 7)", pp.81~92 (2009), Humana Press
- Gowthami R, "Status and consolidated list of threatened medicinal plants of India", Genet Resour Crop Evol, 68, pp.2235~2263 (2021)
- Irimpan MT, "A study of the phytochemical composition and antibacterial activity of Holostemma adakodien Schultes", Int J PharmTech Res, 3(2), pp.1208~1210 (2011)
- Jain PS, "Isolation of lupeol, stigmasterol and campesterol from petroleum ether extracts of woody stem of Wrightia tinctoria", Asian J Plant Sci, 9, pp.163~167 (2010)
- Karmarkar SH, "Hairy root induction in adapathiyan (Holostemma ada-kodien K. Schum.)", J Trop Agric, 39, pp.102~107 (2001)
- Kedage V, "Rapid in vitro multiplication protocol for Ceropegia noorjahaniae Ans., a critically endangered, endemic plant of the Western Ghats", Adv Plants Agric Res, 7(2), pp.267~270 (2017)
- Keshavachandran R, "In vitro conservation of endangered medicinal plants", Pro. National Symposium on biotechnological interventions for improvement of Horticulture crops: Issue and strategies, pp.202~203 (2005)
- Khare CP, "Indian Herbal remedies Rational Western Therapy, Ayurvedic and Other" (2004), Springer-Verlag
- Kirtikar KR, "Indian medicinal plants", pp.894~895 (2005), Periodical experts book agency
- Kolammal M, "Pharmacognosy of ayurvedic drugs" (1979)
- Komalavalli N, "In vitro propagation of Gymnema sylvestre- a multipurpose medicinal plant", Plant Cell Tiss Org Cult, 61, pp.97~105 (2000)
- Krishnan PN, "Rapid micropropagation of Woodfordia fruticosa (L) Kurz. (Lythraceae), a rare medicinal plant", Plant Cell Rep, 14, pp.55~58 (1994)
- Lee Y, "Influence of auxins, cytokinins and nitrogen on production of rutin from callus and adventitious roots of white mulberry tree (Morus alba L.)", Plant Cell Tiss Org Cult, 105, pp.9~19 (2011)
- Manikpuri N, "Phytochemical investigation of bioactive constituent of some medicinal plants", Intl Research Journal, 2(13), pp.37~38 (2010)
- Martin KP, "Rapid propagation of Holostemma ada-kodien Schult, a rare medicinal plant through axillary bud multiplication and indirect organogenesis", Plant Cell Rep, 21, pp.112~117 (2002)
- Martin KP, "Plant regeneration through somatic embryogenesis on Holostemma ada-kodien, a rare medicinal plant", Plant Cell Tiss Organ Cult, 72, pp.79~82 (2003)
- Misra N, "In vitro biosynthesis of antioxidants from Hemidesmus indicus R. Br. Cultures", In Vitro Cell Dev Biol Plant, 41, pp.285~290 (2005)
- Pathak AR, "Regeneration and chemical profiling in Hemidesmus indicus (L.) R. Br", South African J Bot, 113, pp.413~420 (2017)
- Pushparajan G, "Studies on medicinal plants, Micropropagation of Holostemma ada-kodien Schult. - A rare medicinal plant", International Journal of Advanced Research, 2, pp.394~399 (2014)
- Raviraja Shetty G, "Medicinal Plants in India: Importance and Cultivation, Chapter 18, Jivanti (Holostemma ada-kodien Schult)", pp.310~323 (2019)
- Sakakibara H, "Interactions between nitrogen and and cytokinin in the regulation of metabolism and development", Trends in Plant Science, 11, pp.440~448 (2006)
- Sivarajan VV, "Ayurvedic drugs and their plant sources. 195th ed", pp.195~ (1994), Oxford and IBM Pub Co Pvt Ltd
- Sreekumar S, "Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde", Plant Cell Tiss Org Cult, 62, pp.211~218 (2000)
- Sudha CG, "In vitro propagation of Holostemma annulare (Roxb.) K. Schum., a rare medicinal plant", Plant Cell Rep, 34(1), pp.57~63 (1998)
- Sudhakaran MV, "Botanical Pharmacognosy of Holostemma ada-kodien Schult", Pharmacogn J, 9(2), pp.163~170 (2017)
- Sunil U, "A review on medicinal plants of Holostemma ada-kodien (family: Asclepiadaceae)", J Pharmacognosy and Phytochemistry, 5(3), pp.273~276 (2016)
- Sunil U, "Bioassay guided isolation and identification of the antioxidant constituent from Holostemma ada-Kodien Shcult", Int J Pharma Bio Sci, 8, pp.1~10 (2017)
- Tuppad S, "A Review on the Pharmacology of Holostemma ada-kodien - A vulnerable medicinal", Plant. Int J Curr Microbiol App Sci, 6, pp.1532~1537 (2017)
- Vedavathy S, "Brown gold cultivation in Western Ghats", Natural Prod Rad, 3, pp.22~23 (2004)
- Veerashree V, "In vitro plant regeneration in Gymnema sylvestre R. Br.: Influence of season and subculture induced oxidative stress", Int J Pure App Biosci, 6(4), pp.276~290 (2018)
- Warrier PK, "Indian medicinal plants, a compendium of 500 species", pp.165~ (1995), Orient Longman