Expression Analysis of Sweetpotato Sporamin Genes in Response to Infection with the Root-Knot Nematode Meloidogyne incognita
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Sweetpotato (Ipomoea batatas [L.]) is a globally important root crop cultivated for food and industrial processes. The crop is susceptible to the root-knot nematode (RKN) Meloidogyne incognita, a major plant-parasitic RKN that reduces the yield and quality of sweetpotato. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to M. incognita infection. Among these, several sporamin genes were identified for RKN resilience. Sporamin is a storage protein primarily found in sweetpotato and morning glory (Ipomoea nil). In this study, transcriptional analysis was employed to investigate the role of sporamin genes in the defense response of sweetpotato against RKN infection in three susceptible and three resistant cultivars. Twenty-three sporamin genes were identified in sweetpotato and classified as group A or group B sporamin genes based on comparisons with characterized sweetpotato and Japanese morning glory sporamins. Two group A sporamin genes showed significantly elevated levels of expression in resistant but not in susceptible cultivars. These results suggest that the elevated expression of specific sporamin genes may play a crucial role in protecting sweetpotato roots from RKN infection.
Abstract
Sweetpotato (Ipomoea batatas [L.]) is a globally important root crop cultivated for food and industrial processes. The crop is susceptible to the root-knot nematode (RKN) Meloidogyne incognita, a major plant-parasitic RKN that reduces the yield and quality of sweetpotato. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to M. incognita infection. Among these, several sporamin genes were identified for RKN resilience. Sporamin is a storage protein primarily found in sweetpotato and morning glory (Ipomoea nil). In this study, transcriptional analysis was employed to investigate the role of sporamin genes in the defense response of sweetpotato against RKN infection in three susceptible and three resistant cultivars. Twenty-three sporamin genes were identified in sweetpotato and classified as group A or group B sporamin genes based on comparisons with characterized sweetpotato and Japanese morning glory sporamins. Two group A sporamin genes showed significantly elevated levels of expression in resistant but not in susceptible cultivars. These results suggest that the elevated expression of specific sporamin genes may play a crucial role in protecting sweetpotato roots from RKN infection.
참고문헌 (29)
- Afuape SO, "Studies on some important consumer and processing traits for breeding sweet potato for varied end-uses", Am J Exp Agri, 4, pp.114~124 (2014)
- Anderson C, "Hydrolytic selectivity of patatin (lipid acyl hydrolase) from potato (Solanium tuberosum L.) tubers towards various lipids", J Food Biochem, 26, pp.63~74 (2002)
- Bridge J, "Plant nematodes of agricultural importance a color handbook", pp.77~78 (2010), Academic Press
- Cai D, "Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots", Plant Mol Biol, 51, pp.839~849 (2003)
- Castagnone-Sereno P, "Diversity and evolution of root knot nematodes, genus Meloidogyne : new insights from the genomic era", Annu Rev Phytopathol, 51, pp.203~220 (2013)
- Diaz JT, "Simultaneous saccharification and fermentation of industrial sweetpotatoes for ethanol production and anthocyanins extraction", Indust Crops Prod, 62, pp.53~60 (2014)
- Galliard T, "The enzymic deacylation of phospholipids and galactolipids in plants : purification and properties of a lipolytic acyl-hydrolase from potato tubers", Biochem J, 121, pp.379~390 (1971)
- Grace MH, "Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties", Food Chem, 145, pp.717~724 (2014)
- Ha J, "Comparative proteomic analysis of the response of fibrous roots of nematode-resistant and-sensitive sweet potato cultivars to root-knot nematode Meloidogyne incognita", Acta Physiol Plant, 39, pp.262~ (2017)
- Hattori T, "High-level expression of tuberous root storage protein genes of sweetpotato in stems of plantlets grown in in vitro on sucrose medium", Plant Mol Biol, 14, pp.595~604 (1990)
- Hattori T, "Structural relationship among the members of multigene family coding for the sweetpotato tuberous roots storage proteins", Plant Mol Biol, 13, pp.563~572 (1989)
- Hirschberg HJHB, "Cloning, expression, purification and characterization of patatin, a novel phospholipase A", Euro J Biochem, 268, pp.5037~5044 (2001)
- Hou WC, "Antioxidant activities of a 33KDa root storage protein of sweet potato(Ipomoea batatas(L. )Lam cv. Tainong 57)", J Agri Food Chem, 49, pp.2978~2981 (2001)
- Lam) root storage protein, "Plant Science", 1997, 151~158
- Hou WC, "Sweet potato (Ipomoea batatas (L.) Lam) trypsin inhibitors, the major root storage proteins, inhibit one endogenous serine protease activity", Plant Science, 163, pp.733~739 (2002)
- Jones JT, "Top 10 plant-parasitic nematodes in molecular plant pathology", Mol Plant Pathol, 14, pp.946~961 (2013)
- Lee IH, "The defense response involved in sweetpotato resistance to root-knot nematode Meloidogyne incognita : Comparison of root transcriptomes of resistant and susceptible sweetpotato cultivars with respect to induced and constitutive defense responses", Front Plant Sci, 12, pp.671677~ (2021)
- Lee IH, "Transcriptome analysis of root-knot nematode(Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars", Planta, 249, pp.431~444 (2019)
- Palomares-Rius JE, "Omics fields of study related to plant-parasitic nematodes", J Integ Omics, 3, pp.1~10 (2013)
- Park SC, "Stable internal reference genes for the normalization of real-time PCR in different sweetpotato cultivars subjected to abiotic stress conditions", Plos One, 7, pp.e51502~ (2012)
- Racusen D, "Esterase specificity of patatin from two potato cultivars", Can J Bot, 64, pp.2104~2106 (1986)
- Senda K, "A cytosolic phospholipase A2 from potato tissues appears to be patatin", Plant Cell Physiology, 37, pp.347~353 (1996)
- Senthilkumar R, "Multiple biological functions of sporamin related to stress tolerance in sweetpotato(Ipomoea batatas Lam)", Biotechnol Adv, 30, pp.1309~1317 (2012)
- Tamura K, "MEGA6 : molecular evolutionary genetics analysis version 6. 0", Mol Biol Evol, 30, pp.2725~2729 (2013)
- Tonon C, "An acidic β-1, 3 glucanase from potato tubers appears to be patatin", Plant Physiol Biochem, 39, pp.849~854 (2001)
- Van Loon LC, "The families of pathogenesis-related proteins, their activities and comparative analysis of PR-1 type proteins", Physiol Mol Plant Pathol, 55, pp.85~97 (1999)
- Yao PL, "Site directed mutagenesis evidence for a negatively charged trypsin inhibitory loop in sweet potato sporamin", FEBS Lett, 496, pp.134~138 (2001)
- Yeh KW, "Functional activity of sporamin from sweet potato (Ipomoea batatas Lam.): a tuber storage protein with trypsin inhibitory activity", Plant Mol Biol, 33, pp.565~570 (1997)
- Yeh KW, "Sweet potato (Ipomoea batatas Lam.) trypsin inhibitors expressed in transgenic plants confer resistance against Spodoptera litura", Plant Cell Rep, 16, pp.696~699 (1997)